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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.12, no.3, 1999³â, pp.234-239
¼¶¿À°¥Çdzª¹«¿¡¼­ SOD IsoenzymeÀÇ ½Äº° ¹× Ư¼º±Ô¸í
( Identification and Characterization of SOD Isoenzymes in Acanthopanax koreanum Plants )
¿À¼øÀÚ;¹Ú¿µÃ¶;±èÀÀ½Ä;°í¼®Âù; Á¦ÁÖ´ëÇб³ »ý¹°Çаú;Á¦ÁÖµµ ³ó¾÷±â¼ú¿ø;¼­³²´ëÇб³ »ý¹°Çаú;Á¦ÁÖ´ëÇб³ »ý¹°Çаú;
 
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µÎ¸¨³ª¹«°ú ½Ä¹° 6Á¾ÀÇ ÀÙÀ¸·ÎºÎÅÍ 2°³ÀÇ °øÅëÀûÀÎ superoxide dismutase(SOD) isoenzymeÀÌ ±¸ºÐµÇ¾ú´Ù. ÀÌµé °øÅëÀûÀÎ isoenzyme(SOD 4¿Í SOD 6)ÀÇ ÆÐÅÏÀº ¿À°¥ÇÇ¼Ó ½Ä¹° Áß¿¡¼­ ¹Î°¡½Ã¿À°¥Çdzª¹«(A. senticosus for. inermis) ÀÙ¿¡¼­ °¡Àå ´Ù¾çÇÏ¿´°í, ±× Ȱ¼ºÀº ¼¶¿À°¥Çdzª¹«(A. koreanum) ÀÙ¿¡¼­ °¡Àå ³ô¾Ò´Ù. ±×¸®°í SOD 4¿Í SOD 6Àº $H_2O$$_2$¿Í KCN¿¡ ÀÇÇÑ ¼±ÅÃÀû ÀúÇØ·ÎºÎÅÍ °¢°¢ Fe-SOD¿Í CuZn-SODÀÎ °ÍÀ¸·Î ¹àÇôÁ³´Ù. ¶ÇÇÑ, SOD isoenzymeÀÇ ÆÐÅÏÀº ¼¶¿À°¥Çdzª¹«ÀÇ ¼º¼÷ÇÑ ÀÙ°ú ¼öÇÇ, ±ÙÇÇ¿¡¼­ Â÷À̰¡ ¾ø¾úÀ¸³ª ±× Ȱ¼ºÀº Á¶Á÷º°·Î Â÷À̰¡ ³ªÅ¸³µÀ¸¸ç, Fe-SOD´Â ±ÙÇÇ¿¡¼­, CuZn-SODÀº ÀÙ¿¡¼­ »ó´ëÀûÀ¸·Î ³ô°Ô ³ªÅ¸³µ´Ù. ±×¸®°í, CuZn-SOD³ª Fe-SOD ¸ðµÎ 30-4$0^{circ}C$¿¡¼­ ³ôÀº Ȱ¼º À» ³ªÅ¸³»¾úÀ¸³ª ±× ÀÌ»óÀÇ ¿Âµµ¿¡¼­´Â Ȱ¼ºÀÌ ÀúÇØµÇ¾ú´Ù.
The isoenzyme patterns and activities of superoxide dismutase(SOD) were investigated from leaves of Araliaceae plants. Of the eight isoenzymes, two isoenzymes(SOD 4 and SOD 6) were prevalent to leaves of Araliaceae plants. The patterns of these two isoenzymes were most various in the leaves of Acanthopanax senticosus for. inermis, while their activity was highest in the leaves of A. koreanum. These two isoenzymes were respectively identified as Fe-SOD and CuZn-SOD, based on selective inhibition with KCN or$H_2O_2$. The SOD isoenzyme patterns did not differed among stem barks, root barks and leaves of A. koreanum. However, the activities of Fe-SOD and CuZn-SOD were higher in the root bark and in leaves, respectively. Both of Fe-SOD and CuZn-SOD were stable for 1 hr at 30-4$0^{circ}C$, while unstable above 5$0^{circ}C$.
 
Ű¿öµå
Araliaceae;Acanthopanax koreanum;CuZn-SOD;Fe-SOD;Thermal stability;
 
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸÁö / v.12, no.3, 1999³â, pp.234-239
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199911921402318)
¾ð¾î : Çѱ¹¾î
³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø
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