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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.13, no.2, 2000³â, pp.104-110
RAPD ºÐ¼®¿¡ ÀÇÇÑ °¡½Ã¿À°¥ÇÇÀÇ À¯¿¬°ü°è ºÐ¼®
( Intraspecific Relationship Analysis of Eleutherococcus senticosus Max. by RAPD Markers )
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°¡½Ã¿À°¥ÇÇ ¹× ¿À°¥ÇÇÀÇ ¼öÁýÁ¾ °£ÀÇ À¯¿¬°ü°è¸¦ ±¸¸íÇϱâ À§ÇÏ¿© RAPD ºÐ¼®À» ÇÑ °á°ú 10°³ÀÇ primer¸¦ ¼±¹ßÇÏ¿´À¸¸ç G+CÀÇ ¼ö°¡ ¸ðµÎ 60%ÀÌ»óÀ̾ú´Ù. 10°³ÀÇ primer¸¦ »ç¿ëÇÏ¿© ¾òÀ» ¼ö ÀÖ´Â ÃÑ ¹êµå ¼ö´Â 106°³ ¿´À¸¸ç ÀÌÁß monomorphicÇÑ ¹êµå´Â 17.9%¿¡ ÇØ´çÇÏ´Â 19°³¿´À¸¸ç ³ª¸ÓÁö 87°³´Â polymorphicÇÑ °ÍÀ¸·Î ³ªÅ¸³µ´Ù. 10°³ÀÇ primer¸¦ »ç¿ëÇÏ¿© ¾òÀº 106°³ÀÇ ¹êµå¸¦ °¢°¢ ÇϳªÀÇ ÇüÁú(character)·Î º¸¾Æ À̸¦ À¯¿¬°ü°è¸¦ ºÐ¼®ÇÑ °á°ú ¿µ¿ù ¼öÁýÁ¾°ú ÀϺ»Á¾ ¹× Áö¸®»ê ¿À°¥ÇÇ¿Í ¼­¿ï¿À°¥ÇǸ¦ Æ÷ÇÔÇÏ´Â ±º(Group I )°ú ±¹³»Á¾°ú ·¯½Ã¾ÆÁ¾, Áß±¹Á¾À» Æ÷ÇÔÇÏ´Â ±º(Group II)À¸·Î ³ª´µ¾îÁ³À¸¸ç genetic distance°ªÀÇ Æò±ÕÀº 0.61À̾ú´Ù. Group IÀ¸·Î ºÐ·ùµÈ ºÏÇØµµ °¡½Ã¿À°¥ÇÇ´Â ±¹³»ÀÇ °¢ ¼öÁýÁö¿ªÀÇ °¡½Ã¿À°¥Çdzª ·¯½Ã¾Æ °¡½Ã¿À°¥ÇÇ¿Í ¿ø¿¬ÀÇ °ü°èÀÎ °ÍÀ¸·Î ³ªÅ¸³µÀ¸¸ç 2±º¿¡ Æ÷ÇÔµÈ ¼öÁý Áö¿ªÁ¾ °£ÀÇ ¿ø¿¬ °ü°è Áß Ãáõ ¼öÁýÁ¾Àº ±¹³»ÀÇ ´Ù¸¥ Áö¿ªÀÎ Àá°îÀ̳ª űâ»ê ¿À´ë»ê µî°ú ºñ±³ÇÏ¿© ·¯½Ã¾Æ »êÀ̳ª Áß±¹»ê¿¡ ´ëÇÏ¿© ´õ ±Ù¿¬ÀÇ °ü°è¸¦ ³ªÅ¸³»¾ú´Ù.
To analyse the genetic relationship and intraspecific variations among the Eleutherococcus senticosus population, the polymerase chain reaction(PCR) was performed total genomic DNAs of 10 E. senticosus collections by random 10 primers. The genetic diversity and genetic distance among 10 collections of Eleutherococcus spp. were used to describe the dendrogram showing phylogenic relationship. Ten collections were classfied into two group(group I, II) at the similarity coefficient value of 0.50. Group I included E. senticosus of Bukhado(Japanese), youngwal(Korea), E. seoulense, and E. chiisanesis while group II included several internal and Russia collection. The range of polymorphism was from 66.7 to 90.9% in 87 amplified DNA fragments. The similarity value of all collections ranged from 0.41 to 0.92. The average of genetic distance was 0.61.
 
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polymerase chain reaction(PCR);genetic similarity;
 
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸÁö / v.13, no.2, 2000³â, pp.104-110
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200011921402630)
¾ð¾î : Çѱ¹¾î
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