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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.15, no.3, 2002³â, pp.250-259
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RAPD¸¦ ÀÌ¿ëÇÑ Â¤½Å³ª¹°(Agrimonia pilosa Ledeb.) ¼öÁýÁ¾ À¯¿¬°ü°è ºÐ¼®
( Analysis of Genetic Relation among Collected Landraces of Agrimonsa pilosa L. Using RAPD ) |
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| ¿ì¸® ³ª¶ó ÀÚ»ý ¤½Å³ª¹°(Agrimonia pilosa Ledeb.) ¼öÁýÁ¾ÀÇ ºÐÆ÷, ÀÚ»ýÁöÀÇ È¯°æ ¹× ÀÚ»ýÁö¿¡¼ÀÇ ÇüÅÂÀû Ư¼ºÁ¶»ç¸¦ ½Ç½ÃÇϰí, ÀÚ»ýÁ¾ ¤½Å³ª¹°¿Í µµÀÔ Â¤½Å³ª¹° °£ÀÇ ÇüÅÂÀû Ư¼º ¹× À¯¿¬°ü°è¸¦ ¿¬±¸ÇÑ °á°ú´Â ´ÙÀ½°ú °°´Ù. 1. ¼öÁýµÈ Á¾ Áß¿¡¼ Çϵ¿Áö¿ªÀÇ È°³¸é ½Ö°è»ç¿Í û¾Ï¸é ûÇе¿¿¡¼ ¼öÁýµÈ 2Á¾Àº ´Ù¸¥ ¼öÁýÁ¾¿¡ ºñÇÏ¿© À¯ÀüÀû °Å¸®Áö¼ö°¡ °¡Àå °¡±î¿î ¼öÄ¡¿¡¼ ³ªÅ¸³ª ±Ù¿¬°ü°è·Î ³ªÅ¸³µÀ¸¸ç, ÃæºÏ ¿µµ¿À¾ ºñźÀç¿Í °æ±âµµ °ÈÀ¾ ¼öÁýÁ¾°£¿¡´Â À¯ÀüÀû °Å¸®Áö¼ö´Â ÃÖ´ë°ªÀÌ °°Àº °ÍÀ¸·Î º¸¾Æ À¯¿¬°ü°è°¡ ÀÖ´Â °ÍÀ¸·Î ÆÇ´ÜµÈ´Ù. 2. °æ³²°ÅÁ¦¿¡¼ ¼öÁý(No. 7)µÈ °Í°ú ÃæºÏ º¸Àº¿¡¼ ¼öÁý(No. 21)µÈ 2Á¾ÀÌ À¯ÀüÀûÀ¸·Î °¡Àå °¡±õ°Ô ³ªÅ¸³ª Áö¿ªÀû Â÷ÀÌ¿¡ ÀÇÇÑ ÁøÈ¿¡µµ ´Ù¾çÇÔÀ» º¸À̰í ÀÖ¾î ¼öÁýÁö¿ªÀÇ È®´ë¿Í ÀÌ¿¡ µû¸¥ ü°èÀûÀÎ ¼±¹ßÀ» ÅëÇØ¼ ¿ì·®Á¾ÀÇ ¼±¹ßµµ °¡´ÉÇÒ °ÍÀ¸·Î ÆÇ´ÜµÈ´Ù 3.ÁõÆøµÈ DNA band pattern¿¡ ÀÖ¾î¼ ÀýÆíÅ©±â´Â 300¡21000p·Î PCR·Î ÁõÆøµÈ Á¾ »óÈ£°£ÀÇ 1-F°ªÀº Æò±Õ 0.624¿´°í, ÃÖ¼Ò°ª 0.365, ÃÖ´ë°ª 0.827À̾úÀ¸¸ç, 2°³ ±ºÀ¸·Î ºÐ¸®µÇ¾î À¯ÀüÀûÀ¸·Î »ó´çÇÑ Â÷À̰¡ ÀÖ´Â °ÍÀ¸·Î ³ªÅ¸³µ´Ù. |
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| Agromonia pilosa Ledeb. has been used as a medicinal plant in traditional folk remedy. There are few reports on classification, physiology, ecology and morphological studies of Agromonia pilosa L. in Korea. Therefore, advanced approaches on study and development with this plant would be done urgently. Present stndy was carried out to gain basic information on genetic resources and variation with collected domestic landraces through RAPD analysis in Agromonia pilosa L. Forty two collections of Agromonia pilosa L. from nation-wide area including USA one were analyzed by RAPD test. Molecular marker size by amplified DNA band pattern ranged from 300 to 2,100bp. Among the collection, two landraces of Hadong and Cheonghak-dong showed close relation in genetic similarity. Minimum and maximum value by matrix of 1-F among 26 collected landraces were figured out as 0.365 and 0.827 showing mean value for 0.624, respectively. Those landraces were classified into two groups with cluster analysis by Nei and Li's formula from RAPD-analyzed values, and considerable genetic differences were recognized between two groups. |
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| Agromonia pilosa Ledeb.;Genetic relation;RAPD; |
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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸÁö / v.15, no.3, 2002³â, pp.250-259
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200211921975051)
¾ð¾î : Çѱ¹¾î |
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| ³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø |
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