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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.16, no.1, 2003³â, pp.55-60
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ÀÚ¿õÀÌÁÖ ½Ä¹° ¼ö¿µ (Rumex acetosa L.)¿¡¼ ¾Ï.¼ö¿¡ µû¸¥ RAPD patternÀÇ ´Ù¾ç¼º ºÐ¼®
( Variation of RAPD patterns between Male and Female Genomic DNAs in Dioecious Rumex acetosa L. ) |
| ±èµ¿¼ø;±¸´Þȸ;ÇãÀ±°;¹æÀç¿í; Ãæ³²´ëÇб³ ÀÚ¿¬°úÇдëÇÐ »ý¹°Çаú;Ãæ³²´ëÇб³ ÀÚ¿¬°úÇдëÇÐ »ý¹°Çаú;Ãæ³²´ëÇб³ ÀÚ¿¬°úÇдëÇÐ »ý¹°Çаú;Ãæ³²´ëÇб³ ÀÚ¿¬°úÇдëÇÐ »ý¹°Çаú;
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| ¾Ï$.$¼ö °³Ã¼ »çÀÌ¿¡ »óÀÌÇÑ ¼º¿°»öü Á¶¼ºÀ» Áö´Ï´Â ÀÚ¿õ ÀÌÁÖ ½Ä¹°ÀÎ ¼ö¿µ (Rumex acetsa L.)¿¡¼ 120°³ ÀÇ 10-mer random primer¸¦ ÀÌ¿ëÇÏ¿© RAPD ºÐ¼®À» ¼öÇàÇÏ¿´´Ù. Àû¿ëÇÑ primerµé Áß 24°³ÀÇ primer ¿¡¼ 34°³ÀÇ ¾Ï$.$¼ö ƯÀÌ ¹êµå°¡ °üÂûµÇ¾ú´Ù ¾Ï °³Ã¼ ƯÀÌ ¹êµå´Â 16°³¿´À¸¸ç, ¼ö °³Ã¼ ƯÀÌ ¹êµå´Â 18°³·Î ³ªÅ¸³µ´Ù. ƯÈ÷ OPC-10 primer·ÎºÎÅÍ ¾òÀº 1,440 bpÀÎ DNA ´ÜÆíÀº ¼ö °³Ã¼ ƯÀÌÀûÀ¸·Î ³ªÅ¸³µ´Ù. º» ¿¬±¸¿¡¼ ¾ò¾îÁø ¼º ƯÀÌÀûÀÎ RAPD ¸¶Ä¿µéÀº ½Ä¹°¿¡¼ ¼º °áÁ¤ ¸ÞÄ¿´ÏÁò ±¸¸íÀÇ ±âº» ÀÚ·á·Î Ȱ¿ëµÉ ¼ö ÀÖÀ» °ÍÀÌ´Ù. |
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| The genetic variation of random amplified polymorphic DNA (RAPD) patterns of genomic DNAs was investigated in dioecious plant Rumex acetosa L., which carries different sex chromosome complements in female (2n=12A+XX) and male (2n=12A+XY$_1$Y$_2$). One hundred and twenty random primers consisted of 10-mer were used for PCR amplification. Polymorphic bands were found in 24 primers. Specific bands for female and male were 16 and 18, respectively. Especially, a band of 1,440 bp from the OPC-10 primer was male specific. These sex specific RAPD markers are used to understanding the sex determination mechanism in plants. |
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| Ű¿öµå |
| polymorphism;RAPD;Rumex acetosa;sex chromosome; |
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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸÁö / v.16, no.1, 2003³â, pp.55-60
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200311921975207)
¾ð¾î : Çѱ¹¾î |
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| ³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø |
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