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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.16, no.3, 2003³â, pp.251-256
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RAPD marker¸¦ ÀÌ¿ëÇÑ °í·ÁÀλï(Panax ginseng C.A.Meyer)ÀÇ À¯ÀüÀû º¯ÀÌ ºÐ¼®
( Genetic Variation in Among Cultivated Field Populations of Korean Ginseng(Panax ginseng C.A.Meyer) Using RAPD ) |
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| º» ¿¬±¸´Â °í·ÁÀλïÀÇ Áý´Ü³»ÀÇ À¯ÀüÀû º¯À̸¦ ÀÛ¹°ÇÐÀû Ư¼º ¹× DNA¼öÁØ¿¡¼ ºñ±³ÇÏ¿© ÀλïǰÁ¾À°¼ºÀ» À§ÇÑ ±âÃÊ ÀڷḦ Á¦°øÇϱâ À§ÇÏ¿© ¼öÇàÇÑ °á°ú¸¦ ¿ä¾àÇÏ¸é ´ÙÀ½°ú °°´Ù. 1.°³È±â´Â 5¿ù 16ÀϺÎÅÍ 24ÀÏ ±îÁö ÀÏÁÖÀÏ¿¡ °ÉÃÄ °³ÈÇÏ¿´°í, 19Àϰú 22ÀÏ¿¡ °³ÈÀ²ÀÌ °¡Àå ºóµµ°¡ ³ô¾Ò´Ù. 2. Áٱ⠻öÀº ³ì»öÀÌ 13°³Ã¼, ¿¬ÀÚ»öÀÌ 429°³Ã¼, ÀÚ»öÀÌ 229°³Ã¼, ÁøÀÚ»öÀÌ 38°³Ã¼·Î ¿¬ÀÚ»öÀÌ °¡Àå ¸¹Àº ºÐÆ÷¸¦ º¸¿´À¸¸ç ÇÑ Áý´Ü ³»¿¡¼ ´Ù¾çÇÑ ºÐÆ÷¸¦ ³ªÅ¸³Â´Ù. 3. ÃÊÀåÀº 22¡©68cmÀÇ ³ÐÀº ¹üÀ§¿¡ ºÐÆ÷ÇÏ¿´À¸¸ç, 20¡©27cm°¡ 31°³Ã¼ , 27¡©34cm°¡ 88°³ ü, 34¡©41cm°¡ 219°³Ã¼ , 41¡©48cm°¡ 291°³Ã¼, 48¡©55cm°¡ 63°³Ã¼, 55¡©62cm°¡ 10°³Ã¼, 62¡©69cm°¡ 5°³Ã¼·Î ´Ù¾çÇÑ ºÐÆ÷¸¦ ³ªÅ¸³Â´Ù. 4. »Ñ¸®¹«°Ô´Â 16¡©26gÀÌ 53°³Ã¼, 26¡©36gÀÌ 137°³Ã¼, 36¡©46gÀÌ 385°³Ã¼, 46¡©56gÀÌ 94°³Ã¼, 56¡©66gÀÌ 27°³Ã¼, 66¡©76gÀÌ 9°³Ã¼, 76¡©86gÀÌ 4°³Ã¼, 36¡©46g¹üÀ§¿¡¼ 385(57.7%)°³Ã¼·Î °¡Àå ¸¹Àº ºÐÆ÷¸¦ ³ªÅ¸³»¾úÀ¸¸ç, 16¡©86gÀÇ ¹üÀ§·Î º¯ÀÌ Á¤µµ°¡ ¸Å¿ì ÄÇ´Ù. 5.662°³Ã¼¸¦ ´ë»óÀ¸·Î RAPD¸¦ ½Ç½ÃÇÑ °á°ú 32°³ÀÇ ÇÁ¶óÀÌ¸Ó Áß 10°³ °¡ ÀçÇö¼ºÀÌ°í ´ÙÇü¼ºÀÎ ¹êµå¸¦ º¸¿´´Ù. 10°³ÀÇ ÇÁ¶óÀ̸ӿ¡¼ ³ªÅ¸³ Àüü ¹êµå¼ö´Â 109°³¿´À¸¸ç ÀÌÁß 103°³°¡ ´ÙÇü¼ºÀ» º¸¿© ´ÙÇü¼º ºñÀ²Àº 94.5%¿´´Ù. 6. URP5 ÇÁ¶óÀ̸Ӹ¦ ÀÌ¿ëÇÏ¿© 662°³Ã¼¸¦ ±ºÁý ºÐ¼®ÇÑ °á°ú ¹êµåÀÇ À¯¹«¿¡ µû¶ó 16°³ÀÇ ±×·ìÀ¸·Î ±¸ºÐÇÏ¿´À¸¸ç, °³È±â, ÃÊÀå, ÁÙ±â»ö, »Ñ¸®Á÷°æ, »Ñ¸®¹«°Ô¿¡ ´Ù¸¥ ºÐ·ù¿Í ÀÏÄ¡ÇÏÁö ¾Ê¾Ò´Ù. |
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| Genetic variation in field grown Korean ginseng(Panax ginseng C.A.Meyer) was evaluated using random amplified polymorphic(RAPD) markers. (This experiment was carried to collect the local native from farm of Chungnam National University in Korea in order to investigate genetic variation.) Some morphological characters showed considerable variation ranging 22 to 68cm in plant hight, 10 to 38mm in root diameter, 16 to 86g in root weight, and culum color and flowering date, respectively. Ten RAPD primers out of the 32 which produced reproducible bands in 662 Korean ginseng plants were selected for the further study. The total number of bands generated by 10 primers were 108 and among them 103 were polymorphic among the 662 plants with the polymorphism ratio of 94.5£¥. A total of 662 plants were classified into 16 groups based on polymorphic data with an URP 05 primer. |
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| RAPD;genetic variation;genetic polymorphism;panax ginseng; |
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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸÁö / v.16, no.3, 2003³â, pp.251-256
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200311922145553)
¾ð¾î : Çѱ¹¾î |
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| ³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø |
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