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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.17, no.1, 2004³â, pp.7-13
Àλï õdzÀÇ ºÎÁ¤±Ù ¹è¾çÀ» ÅëÇÑ Ginsenosides »ý»ê
( Ginsenosides Production through in vitro Culture of Adventitous Roots Induced from Panax ginseng "Chunpoong" )
ÀÎÁر³;À̹ü¼ö;¼Û¿ø¼·;¾ç´öÃá; (ÁÖ)¹ÙÀÌ¿ÀÇǾÆ;(ÁÖ)¹ÙÀÌ¿ÀÇǾÆ;¼øÃµ´ëÇб³ ³ó¾÷»ý¸í°úÇдëÇÐ;°æÈñ´ëÇб³ ÇѹæÀç·á°¡°ø¼¾ÅÍ;
 
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Àλï õdzÀÇ ¹è(embryo)·ÎºÎÅÍ ºÎÁ¤±ÙÀ» À¯µµÇÏ°í ¹è¾ç¹èÁöÁ¶¼º, ½Ä¹° È£¸£¸ó°ú Àλ￰ÀÇ ³óµµ°¡ ÀλïºÎÁ¤±ÙÀÇ »ýÀå°ú ginsenosidesÀÇ »ý¼º¿¡ ¹ÌÄ¡´Â ¿µÇâÀ» Á¶»çÇÏ¿´´Ù. ¹èÁö Á¾·ùº° ÀÎ»ï ºÎÁ¤±ÙÀÇ »ýÀå·®Àº B5, WPM, MS ¹èÁö ¼øÀ¸·Î ³ô¾ÒÀ¸¸ç, ginsenosides ÇÔ·®Àº B5¹èÁö¿¡¼­ °¡Àå ³ô¾ÒÀ¸¸ç panaxa triol°è »çÆ÷´Ñ º¸´Ù panaxa diol°è »çÆ÷´ÑÀÌ Áõ°¡ÇÏ¿´´Ù. ½Ä¹° »ýÀåÁ¶ÀýÁ¦ 󸮱¸¿¡¼­ÀÇ ÀλïºÎÁ¤±ÙÀÇ »ýÀå·®Àº kinetin 0.1 mg/L¿¡¼­ ³ô°Ô ³ªÅ¸³µÀ¸¸ç , ginsenosides ÇÔ·®Àº kinetin 0.01 mg/L¿¡¼­ ¿ÀÈ÷·Á ³ô°Ô ³ªÅ¸³µ´Ù. ƯÈ÷ kinetin 0.01 mg/L ÷°¡¿¡¼­ ginsenoside Rb$_1$ÀÇ ÇÔ·®ÀÌ Å©°Ô Áõ°¡ÇÏ¿´´Ù. KH$_2$PO$_4$¸¦ Ãß°¡ ÷°¡ ÇÒ¶§¿¡´Â ºÎÁ¤±ÙÀÇ »ýÀå¿¡ 2.5 mM¿¡¼­ ´ëÁ¶±¸¿¡ ºñÇØ ¾çÈ£ÇÏ¿´À¸³ª, ginsenosidesÀÇ ÇÔ·®Àº 1.25 mM¿¡¼­ ³ô¾ÒÀ¸¸ç , ƯÈ÷ ginsenosides ReÀÇ ±Þ°ÝÇÑ Áõ°¡·Î ÀÎÇÏ¿© panaxatriol°è »çÆ÷´ÑÀÇ ÇÔ·®ÀÌ Áõ°¡µÇ¾ú´Ù.
We have investigated the optimal conditions for the growth and ginsenoside production in adventitious root of ¡°Chunpoong¡± (Panax ginseng C.A. Meyer). Ginseng adventitous roots induced from the embryo of ¡°Chunpoong¡± were cultured in various plant media supplemented with several growth hormones. The best growth rate and high ginsenoside contents were obtained in B5 medium supplemented with IBA (2 mg/L) and kinetin (0.1 mg/L). The supplement of 2.5 mM KH$_2$PO$_4$ was good for high growth rate of the adventitious roots, but the accumulation of ginsenosides was increased by reducing the KH$_2$PO$_4$ concentration to 1.25 mM. We have established the effective liquid culture system for the optimal growth and ginsenoside production of ¡°Chunpoong¡± adventitious roots.
 
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Adventitous roots;chunpoong;ginsenosides;Panax ginseng;
 
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸÁö / v.17, no.1, 2004³â, pp.7-13
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200411922290319)
¾ð¾î : Çѱ¹¾î
³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø
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