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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.17, no.1, 2004³â, pp.43-47
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ÇѶó±¸ÀýÃÊ ÀÙÀýÆí ¹è¾ç¿¡ ÀÇÇÑ ½Ä¹°Ã¼ ÀçºÐÈ
( Plant Regeneration from Leaf Segment Culture of Chrysanthemum zawadskii ssp. coreanum ) |
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| ±â³»¿¡¼ »ð¸ñ ¹è¾çÁßÀÎ ÇѶó±¸ÀýÃÊ[( Chrysanthemum zawadskii ssp. coreanum(Nakai) Y.N. Lee)]ÀÇ ÀÙÀ¸·ÎºÎÅÍ È¿°úÀûÀÎ ½Ä¹°Ã¼ Àç»ýÀ» À§ÇÑ NAA¿Í BA³óµµ ¹× ¹èÁö°íÇü¹°ÀÇ È¿°ú¸¦ Á¶»çÇÏ¿´´Ù. 0.2% Gelite¸¦ °íÇü¹°·Î ÇÏ¿´À» ¶§ ÇѶó±¸ÀýÃÊÀÇ ÀÙ ÀçºÐÈ¿¡´Â BA³óµµº¸´Ù´Â NAA ³óµµ°¡ ´õ ¿µÇâÀ» ¹ÌÃÆÀ¸¸ç, NAA ³óµµ°¡ 1 mg/L ÀÌ»óÀÌ¸é ¸ðµç Á¶Á÷ ¿¡¼ ÀçºÐȸ¦ º¸¿´´Ù. ½ÅÃʼö´Â NAA ´Ü¿ë 󸮿¡¼ ¸¹Àº °æÇâÀ̾úÀ¸¸ç NAA 1 mg/L 󸮽ð¡ °¡Àå ¾çÈ£ÇÏ¿´´Ù. ¹èÁö°íÇü¹°·Î agar°ú gelite¸¦ °¡Áö°í ÀçºÐÈ È¿°ú¸¦ Á¶»çÇÑ °á°ú, gelite ÷°¡½Ã ÀÙÀýÆí ´ç ½ÅÃʼö°¡ ¸¹¾ÒÀ¸¸ç agar ÷°¡½Ã¿¡´Â ÀÙÀýÆí´ç ½ÅÃʼö°¡ ÀûÀº ¹Ý¸é¿¡ ½ÅÃÊÀÇ ±æÀ̰¡ ±æ°Ô ³ªÅ¸³µ´Ù. ÇѶó±¸ÀýÃÊÀÇ ÀÙÀýÆíÀ¸·ÎºÎÅÍ ÀçºÐÈ¿¡´Â MS¿¡ 3% sucrose, 0.8% agar¿Í NAA 1 mg/L¸¦ ÷°¡ÇÏ¿© 25 $^{circ}C$, 2,000 lux Á¤µµ¿¡¼ ¹è¾çÇÏ´Â °ÍÀÌ È¿°úÀûÀ̾ú´Ù. |
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| The effect of plant growth regulators and gelling agents for plant regeneration from leaf segment of Chrysanthemum zawadskii ssp. coreanum was investigated. NAA was more effective than BA for plant regeneration. MS medium supplemented with NAA 1 mg/L was the most effective in plant regeneration. The effect of agar and gelite as gelling agent was compared. Agar(0.8%) was more effective than gelite(0.2%) in plant regeneration. Regenerated shoots was successfully increased by shoot grafting in MS medium supplemented with NAA 0.1 mg/L in vitro, and hardened by shoot grafting in artificial soil mix(Peatmoss : Perlite = 1 : 1). |
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| Ű¿öµå |
| Chrysanthemum zawadskii ssp. coreanum;leaf;plant regeneration; |
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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸÁö / v.17, no.1, 2004³â, pp.43-47
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200411922290408)
¾ð¾î : Çѱ¹¾î |
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| ³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø |
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