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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.17, no.2, 2004³â, pp.153-160
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°¹¹æÇ³(Glehnia littoralis Schmidt et Miquiel)¿¡¼ Ķ·¯½º À¯µµ ¹× ½Ä¹°Ã¼ ÀçºÐÈ¿¡ ¹ÌÄ¡´Â »ýÀåÁ¶ÀýÁ¦ÀÇ È¿°ú
( Comparative Effect of Plant Growth Regulators on Callus Induction and Plant Regeneration in Glehnia littoralis Schmidt et Miquiel ) |
| À̺´±¹;Çѹ̼÷;Á¤¾ç±Õ;³ªÀǽÄ;À±¼ºÁß;À¯³²Èñ; ÀüºÏ´ëÇб³ »ý¹°ÀÚ¿ø°úÇкÎ, (ÁÖ)Ç÷£³Ý;ÀüºÏ´ëÇб³ »ý¹°ÀÚ¿ø°úÇкÎ, (ÁÖ)Ç÷£³Ý;ÀüÁֳ󸲰íµîÇб³;¼øÃµ´ëÇб³ ½Ä¹°»ý»ê°úÇкÎ;ÀüºÏ´ëÇб³ »ý¹°ÀÚ¿ø°úÇкÎ;(ÁÖ)Ç÷£³Ý;
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| °¹¹æÇ³ÀÇ Ä¶·¯½º À¯µµ¿Í ½Ä¹°Ã¼ ÀçºÐÈ¿¡ ¿µÇâÀ» ¹ÌÄ¡´Â »ýÀåÁ¶ÀýÁ¦¿¡ ´ëÇÑ ¸î °¡Áö ¿äÀεéÀ» Á¶»çÇÏ¿´´Ù. ½Ä¹°Ã¼ Á¶Á÷À¸·ÎºÎÅÍ Ä¶·¯½ºÀÇ À¯µµ¿¡ ÀûÀýÇÑ ¹èÁöÀÇ Á¶°ÇÀº 1¡2 mg/L, 2,4-D¿Í 1¡2 mg/L BA È¥¿ëó¸® Á¶ÇÕ¿¡¼ Ķ·¯½ºÀÇ À¯µµÀ²ÀÌ ³ô¾Ò°í, Á¶Á÷º° Ķ·¯½º À¯µµÀ²Àº ¿±Á¶Á÷ º¸´Ù ¿±º´¿¡¼ ¿ì¼öÇÏ¿´À¸³ª, ´Ù¸¥ ½Ä¹°ÀÇ ºÎÁ¤¾Æ ¹ß»ý ¾ç»ó°ú ´Þ¸® ¿±Á¶Á÷¿¡¼ ¹ß»ýÇÑ Ä¶·¯½º·ÎºÎÅÍ ºÎÁ¤¾Æ¿Í ºÎÁ¤±ÙÀÇ Çü¼ºÀ» º¸¿´´Ù. Á¶Á÷º° Ķ·¯½ºÀÇ ½Ä¹°Ã¼ ºÐÈÀ²Àº ½Ä¹°»ýÀå Á¶ÀýÁ¦ÀÇ ³óµµ°¡ ³ôÀ»¼ö·Ï ¾çÈ£ÇÏ¿´°í ÀûÁ¤³óµµ´Â 1 mg/L NAA¿Í 2 mg/L BA À̾ú´Ù. ÀÌÈÄ ºÐÈµÈ ½Ä¹°Ã¼¸¦ °è´ë¹è¾çÇÏ¿© ´ë·®Áõ½ÄÀ» À§ÇØ multiple shoot¸¦ ¹ß»ý½ÃŰ°í »Ñ¸®¸¦ ¹ß»ý½Ãų ¼ö ÀÖµµ·Ï ¹ß±Ù¹èÁö¿¡ ¿Å°Ü »ýÀ° ½ÃŲ ÈÄ ½Ä¹°Ã¼ÀÇ ÃÊÀå¿¡ µû¶ó vermiculite¿¡ ¼øÈ½ÃÄÑ »ýÁ¸À²À» Á¶»çÇÑ °á°ú ³ôÀº »ýÁ¸À²À» º¸ÀÓÀ» ¾Ë ¼ö ÀÖ¾ú´Ù. |
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| Glehnia littoralis is known as an edible and medicinal plant using green loaves and mature roots of plant. In the present paper, the influence of plant growth regulators on callus induction and plant regeneration was investigated. Callus induction and regeneration occurred from leaf and petiole explants in Glehnia littoralis. Optimal condition of plant growth regulators for callus induction from leaf and petiole explants was MS basal medium supplemented with 2mg/L 2,4-D and 2mg/L BA. The frequency of callus induction was higher in petiole explant than leaf. When the callus was cultured on MS basal medium supplemented with 0¡1 mg/L IAA, 0¡1mg/L NAA and 0¡2mg/L BA for about 65 days, the most effective plant growth regulators on plant regeneration from callus were 1mg/L NAA and 2mg/L BA. The plantlets acclimatized successfully and grown in vermiculite matrix. |
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| Ű¿öµå |
| Glehnia littoralis;medicinal plant;NAA;IAA;BA;2,4-D;leaf;petiole; |
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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸÁö / v.17, no.2, 2004³â, pp.153-160
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200411922350847)
¾ð¾î : Çѱ¹¾î |
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| ³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø |
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