|
|
|
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.18, no.3, 2005³â, pp.359-366
|
Neutron ºöÁ¶»ç ´ã¹è ¹× º½Ä¹°Ã¼ÀÇ Æ¯¼º
( Characteristics of Tobacco and Rice Plants Irradiated with Neutron Beam ) |
| äÁ¾¼;±èÀçÈ«;¾çŰÇ;·ùÀçÀÏ;ÀÌÈ¿¿¬;¾ç´öÃá;¹èâÈÞ; Çѱ¹ ¿øÀÚ·ÂÀÇÇпø;Çѱ¹ ¿øÀÚ·ÂÀÇÇпø;Çѱ¹ ¿øÀÚ·ÂÀÇÇпø;¼øÃµ´ëÇб³ ³ó¾÷»ý¸í°úÇдëÇÐ ½Ä¹°»ý»ê°úÇкÎ;Á¦ÁÖ´ëÇб³ »ý¸íÀÚ¿ø°úÇдëÇÐ »ý¸í°øÇкÎ;°æÈñ´ëÇб³ ÇѹæÀç·á°¡°ø¼¾ÅÍ;¼øÃµ´ëÇб³ ³ó¾÷»ý¸í°úÇдëÇÐ ½Ä¹°»ý»ê°úÇкÎ;
|
|
|
 |
|
| |
| ÃÊ ·Ï |
| Àç¹èÁ¾´ã¹è(Nicotiana tabacum L. cv.), ¾ß»ýÁ¾´ã¹è (N. plumbaginifolia)¿Í ºÁ¾ÀÚ(Orya sativa L, cv.)¿¡ neutronºöÀ» °¢°¢ Á¶»ç(irradiation)ÇÏ¿© ¹ß¾Æ, ½Ä¹°Ã¼ÀÇ »ýÀå°ú DNAÀÇ º¯ÀÌ¿¡ ¹ÌÄ¡´Â ¿µÇâÀ» °ËÅäÇÏ¿´´Ù. ´ã¹èÁ¾ÀÚ¿Í ºÁ¾ÀÚ¿¡ 90, 180, 270, 360, 450, 540 Gy±îÁö Á¶»ç·Î ¹ß¾ÆÀ²Àº Å©°Ô °¨¼ÒÇÏÁö ¾Ê¾Ò°í, »ýÀå¿¡ ÀÖ¾î À¯ÀÇÀûÀÎ Â÷ÀÌ´Â ¾ø¾ú´Ù. ±×·¯³ª ´ã¹è½Ä¹°Ã¼¿¡¼ ÃÑ 200°³Ã¼ Áß 71°³Ã¼(¾à $36%$)°¡ ÀÙ¿¡¼ ÇüÅ ÀÌ»óÀ» ³ªÅ¸³»¾ú´Ù. ¶ÇÇÑ ÁÙ±â»ö º¯ÀÌü, ¿±»öº¯ÀÌü, ÈÇüº¯ÀÌü¸¦ À¯µµÇÏ¿´´Ù. ÀÌ °á°ú´Â neutronºöÀÌ À¯¿ëÇÑ µ¹¿¬º¯ÀÌ¿øÀ¸·Î¼ °¡´É¼ºÀÌ ÀÖÀ½À» ½Ã»çÇØ ÁØ´Ù. NeutronºöÀ» Á¶»çÇÑ ÈÄ »ýÀåÇÑ ´ã¹è½Ä¹°Ã¼ÀÇ ÀÙ¿¡ ´ëÇÏ¿© ÃÑ 34°³ÀÇ primer¸¦ ÀÌ¿ëÇÏ¿© RAPD ºÐ¼®ÇÑ °á°ú 20°³ÀÇ primer¿¡¼ ÃÑ 104°³ÀÇ DNA ´ÜÆíÀÌ ÁõÆøµÇ¾ú°í, Áß¼ºÀÚ ºöÁ¶»ç󸮱¸¿¡¼¸¸ ÃâÇöÇÏ´Â DNA ´ÜÆíÀº ³ªÅ¸³ªÁö ¾Ê¾Ò´Ù. |
|
| Effects of neutron beam irradiation on seed germination, growth and RAPD pattern of tobacco (Nicotiana tabacum L. cv.; N. plumbaginifolia) and rice (Orya sativa L. cv.) plants were estimated. Seed germination rate was not significantly changed by the neutron beam treatment in both tobacco and rice seeds. And there was no significant differance in growth of the plants by the neutron beam treatment. Interestingly, however, some of morphological changes, including leaf shape (about $36%$), stem color and leaf color were observed in neutron beam treated tobacco plants. In addition, abnormal flower in petal was observed in the neutron beam treated plant. This results indicate that neutron beam is able to use as an effective mutagen in plant mutations. Scorable products from 20 primers were obtained by RAPD analysis in the leaves of the beam irradiated tobacco plants and most of the plants showed the similar band patterns. |
| |
| Ű¿öµå |
| Germination;neutron beam;Nicotiana plumbaginifolia;Oryza sativa;RAPD; |
| |
|
|
 |
|
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸÁö / v.18, no.3, 2005³â, pp.359-366
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200507523329962)
¾ð¾î : Çѱ¹¾î |
|
| ³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø |
|
|
|
|
|
|