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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.18, no.3, 2005³â, pp.374-381
°í·ÁÀλïÀÇ Ribulose-1,5-Bisphosphate Carboxylase Small Subunit(rbcS) À¯ÀüÀÚÀÇ ºÐ¸® ¹× Ư¼ººÐ¼®
( Molecular Cloning of a cDNA Encoding Ribulose-1,5-bisphosphate Carboxylase Small Subunit (rbcS) from Panax ginseng C. A. Meyer )
ÀÎÁر³;À̹ü¼ö;À±ÀçÈ£;¼ÕÈ­;ÀÌÅÂÈÄ;¾ç´öÃá; (ÁÖ)¹ÙÀÌ¿ÀÇÇ¾Æ »ý¸í°øÇבּ¸¼Ò;(ÁÖ)¹ÙÀÌ¿ÀÇÇ¾Æ »ý¸í°øÇבּ¸¼Ò;°æÈñ´ëÇб³ ÇѹæÀç·á°¡°øÇаú;°æÈñ´ëÇб³ ÇѹæÀç·á°¡°øÇаú;°æÈñ´ëÇб³ ÇѹæÀç·á°¡°øÇаú;°æÈñ´ëÇб³ ÇѹæÀç·á°¡°øÇаú;
 
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°í·Á Àλï(Panax ginseng)ÀÇ »Ñ¸®·ÎºÎÅÍ ribulose-1,5-bisphosphate carboxylase small subunit(rbcS) À¯ÀüÀÚ¸¦ ¼±¹ßÇÏ¿© sequence ºÐ¼®À» ½Ç½ÃÇÏ¿´´Ù. °í·Á Àλï rbcS cDNA´Â 790 bp ¿°±â·Î ±¸¼ºµÇ¾î ÀÖÀ¸¸ç, 183°³ÀÇ ¾Æ¹Ì³ë»ê(pI 8.37)À» ÄÚµåÇÏ´Â 549 bpÀÇ ORF¸¦ °¡Áö°í ÀÖ°í ´Ü¹éÁúÀÇ ºÐÀÚ·®Àº 20.5 kDaÀ¸·Î ÃßÁ¤µÇ¾ú´Ù. Àλï rbcS´Â ±âÁ¸¿¡ º¸°íµÈ °Í°ú À¯»ç¼ºÀ» ³ªÅ¸³»¾úÀ¸¸ç, Helianthus annuus(CAA68490)¿¡¼­ ºÐ¸®µÈ °Í°ú $78%$ÀÇ ³ôÀº »óµ¿¼ºÀ» º¸¿´´Ù. ±âÁ¸¿¡ µ¥ÀÌÅͺ£À̽º¿¡ ÃàÀûµÇ¾î ÀÖ´Â ´Ù¸¥ ½Ä¹°Ã¼·ÎºÎÅÍ ºÐ¸®µÈ rbcS¿Í ¾Æ¹Ì³ë»ê ¼­¿­À» ºñ±³ÇÑ °á°ú ÀλïÀÇ ybcS´Â H. annuus (CAA68490), C. morifolium (AAO25119), L. sativa (Q40250)¿Í ¹ÐÁ¢ÇÑ À¯¿¬°ü°è¿¡ ÀÖ´Â °ÍÀ¸·Î Á¶»çµÇ¾ú´Ù.
A full-length cDNA encoding ribulose-1,5-bisphosphate carboxylase small subunit (rbcS) has been isolated and its nucleotide sequence determined from root in ginseng plant (Panax ginseng). The rbcS cDNA of ginseng is 790 nucleotides long and has an open reading frame of 549 bp with deduced amino acid of 183 residues (pI 8.37), 20.5 kDa. The deduced amino acid sequence of rbcS matched to the previously reported rbcS protein genes and showed a high similarity with the $78%$ identity with rbcS of Helianthus annuus (CAA68490). In the phylogenetic analysis based on the amino acid residues, the ginseng rbcS was clustered with H. annuus (CAA68490), C. morifolium (AA025119) and L. sativa (Q40250).
 
Ű¿öµå
Panax ginseng;rbcC;ribulose-1,5-bisphosphate carboxylase;root;
 
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸÁö / v.18, no.3, 2005³â, pp.374-381
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200507523330078)
¾ð¾î : Çѱ¹¾î
³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø
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