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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.18, no.3, 2005³â, pp.382-389
¾Æ¸¶¸±¸®½º (Hippeastrum hybridum Hort. 'Dazzler') ¼ÒÈ­°æ ¹è¾ç¿¡ ÀÇÇÑ È¿À²Àû ±â³»¹ø½Ä
( Effective In Vitro Propagation from Pedicel Culture of Hippeastrum hybridum Hort. 'Dazzler' )
±è¸íÁØ;±è¿µ¼÷;±èÇö¼ø;°íÁ¤¾Ö; ÀüºÏ´ëÇб³ ³ó¾÷»ý¸í°úÇдëÇÐ »ý¹°ÀÚ¿ø°úÇкΠ³ó¾÷°úÇбâ¼ú¿¬±¸¼Ò;ÀüºÏ´ëÇб³ ³ó¾÷»ý¸í°úÇдëÇÐ »ý¹°ÀÚ¿ø°úÇкΠ³ó¾÷°úÇбâ¼ú¿¬±¸¼Ò;³óÃÌÁøÈïû ±¹Á¦±â¼úÇù·Â°ú;ÀüºÏ´ëÇб³ ³ó¾÷»ý¸í°úÇдëÇÐ »ý¹°ÀÚ¿ø°úÇкΠ³ó¾÷°úÇбâ¼ú¿¬±¸¼Ò;
 
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¾Æ¸¶¸±¸®½º (Hippeastrum hybridum Hort. 'Dazzler')ÀÇ È¿À²ÀûÀÎ ±â³» ±Þ¼Ó ´ë·® Áõ½Äü°è¸¦ È®¸³½Ã۰íÀÚ °³È­15ÀÏÀü È­·Ú¿Í ºÎÂøµÈ ¼ÒÈ­°æÀ» Àç·á·Î ÇÏ¿© ÀûÇÕÇÑ Ä¡»óüÀÇ ¼±º°°ú Ķ·¯½º ¹× ½Ä¹°Ã¼ ºÐÈ­¿¡ ¹ÌÄ¡´Â ½Ä¹°»ýÀå Á¶Àý¹°ÁúÀÇ È¿°ú¸¦ Á¶»çÇÏ¿´´Ù. ¾î¸° È­·Ú³» ¾à, È­»ç, ÁÖµÎ, È­ÁÖ ¹× ÀÚ¹æÀº ¹è¾ç Áß ½Ä¹°Ã¼ ÀçºÐÈ­¸¦ À§ÇÑ Ä¶·¯½º ¹× ¼ÒÀÚ±¸¸¦ Çü¼ºÇÏÁö ¸øÇÏ¿© ¹è¾çÀç·á·Î ºÎÀûÇÕÇÏ¿´À¸³ª ¼ÒÈ­°æÀýÆí¿¡¼­´Â Ķ·¯½º ¹× ÀÚ±¸°¡ Çü¼ºµÇ¾î Ä¡»óÀç·á·Î ÀûÇÕÇÏ¿´´Ù. ¹èÁö³» ÷°¡µÈ ½Ä¹°»ýÀåÁ¶Àý¹°Áú¿¡ µû¶ó Ä¡»óü¿¡¼­ Á÷Á¢ ÀÚ±¸¸¦ Çü¼ºÇϰųª Ķ·¯½º¸¦ ÅëÇØ ½Ä¹°Ã¼·Î ºÐÈ­µÇ´Â 2°¡Áö °æ·Î·Î Çü¼ºµÇ¾ú´Âµ¥ °³È­ 15ÀÏÀü ¼ÒÈ­°æ ÀýÆíÀ» 0.5 mg/L LAA¿Í 1.0 mg/L BA°¡ È¥¿ëó¸®µÈ MS ¹èÁö¿¡ ¹è¾çÇÏ¿© $100%$ ¼ÒÀÚ±¸Çü¼º ¹× ½ÅÃʰ¡ ºÐÈ­µÇ¾î È¿°úÀûÀ̾úÀ¸¸ç ¼ÒÈ­°æ¿¡¼­ Á÷Á¢ Çü¼ºµÈ ÀÚ±¸ ¶Ç´Â Ķ·¯½º¸¦ ÅëÇØ ºÐÈ­µÈ ½ÅÃÊ´Â ¼º°øÀûÀ¸·Î ½Ä¹°Ã¼·Î ÀçºÐÈ­ µÇ¾ú´Ù.
This study was conducted to establish the system of effective in vitro propagation by various explant sources culture of Bippeastrum hybridum Hort, 'Dazzler'. We tested the effects of optimal explant source, plant growth regulators on bulblet formation and plant regeneration. Callus was readily produced on the different tissues excised from floral buds whereas, bulbs and shoots were formed only on pedicel explants as compared with anthers, styles and ovaries. Pedicel is the best optimal explant for in vitro propagation. Two distinct pathways, organogenesis through callus and direct bulblet formation, could be recognized in pedicel culture. Up to the $80-100%$ of bulblet formation and shoot organogenesis from the pedicel in fifteen days before anthesis were effectively induced by MS medium supplemented with 0.5 mg/L NAA and 1.0 mg/L BA. Plantlet regeneration was successfully achieved from pedicel-derived callus, via shoot bud induction or direct bulblet formation. The bulblets with blooming flower were produced within 2 years.
 
Ű¿öµå
bulblet formation;organogenesis;
 
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸÁö / v.18, no.3, 2005³â, pp.382-389
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200507523330126)
¾ð¾î : Çѱ¹¾î
³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø
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