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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.18, no.3, 2005³â, pp.441-449
°í·ÁÀλï Chlorophyll a/b Binding Protein(Cab) À¯ÀüÀÚÀÇ µ¿Á¤ ¹× ºÐÀÚÀûÀΠƯ¼ººÐ¼®
( Molecular Characterization of a cDNA Encoding Chlorophyll a/b Binding Protein (Cab) from Panax ginseng C. A. Meyer )
ÀÎÁر³;À̹ü¼ö;À±ÀçÈ£;¼ÕÈ­;±è¼¼¿µ;¾ç´öÃá; (ÁÖ)¹ÙÀÌ¿ÀÇÇ¾Æ »ý¸í°øÇבּ¸¼Ò;(ÁÖ)¹ÙÀÌ¿ÀÇÇ¾Æ »ý¸í°øÇבּ¸¼Ò;¼øÃµ´ëÇб³ ³ó¾÷»ý¸í°úÇдëÇÐ;°æÈñ´ëÇб³ ÇѹæÀç·á°¡°øÇаú;°æÈñ´ëÇб³ ÇѹæÀç·á°¡°øÇаú;°æÈñ´ëÇб³ ÇѹæÀç·á°¡°øÇаú;
 
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±¤°èII(PSII)´Â °íµî½Ä¹°ÀÇ chloroplast¿¡¼­ µÎ °³ÀÇ ±¤ÇÕ¼º ¹ÝÀÀÁ᫐ ÁßÀÇ ÇϳªÀÌ´Ù. Chlorophyll a/b ±¤¼öÈ® º¹ÇÕü´Â ±¤°èII¸¦ À§ÇÑ ¾ÈÅ׳ª ¿ªÇÒÀ» ¼öÇàÇÑ´Ù. º» ¿¬±¸¿¡¼­´Â ÀλïÀÇ ÀÙÁ¶Á÷À» Á¦ÀÛÇÑ cDNA library·ÎºÎÅÍ chlorophyll a/b-binding protein (Cab) À¯ÀüÀÚ¸¦ ºÐ¸®ÇÏ¿´´Ù. Àλï CabÀ¯ÀüÀÚ´Â 935 bpÀÇ ¿°±â¿Í 265°³ÀÇ ¾Æ¹Ì³ë»ê Àܱâ(pI 5.63)·Î ±¸¼ºµÈ ÇÑ °³ÀÇ ORF¸¦ Æ÷ÇÔÇϰí ÀÖÀ¸¸ç, ´Ü¹éÁúÀÇ ºÐÀÚ·®Àº 28.6 kDaÀ¸·Î ÃßÁ¤µÇ¾ú´Ù. Àλ£¼­ ºÐ¸®ÇÑ Cab À¯ÀüÀÚ´Â ±âÁ¸¿¡ ½Ä¹°¿¡¼­ º¸°íµÈ À¯ÀüÀÚµé°ú À¯»ç¼ºÀ» ³ªÅ¸³»¾úÀ¸¸ç, À¯»çµµ´Â $68-92%$·Î ³ªÅ¸³µ´Ù. ¾Æ¹Ì³ë»ê ¼­¿­À» ºñ±³ÇÏ¿© À¯¿¬°ü°è¸¦ ºÐ¼®ÇÑ °á°ú ÀλïÀÇ Cab À¯ÀüÀÚ´Â ºñ±³µÈ P. persica (AAC34983), A.thaliana (AAD28771), G. hirsutum (CAA38025), G. max (AAL29886), V. radiata (AAF89205) µî°ú µ¿ÀÏÇÑ ±×·ìÀ¸·Î ºÐ¸®µÇ¾ú´Ù.
Photo system II (PSII) is one of the two photosynthetic reaction centers in the chloroplast of higher plants. The chlorophyll a/b-light harvesting complex serves primarily as an antenna for PSII. We isolated a cDNA that encodes a chlorophyll a/b-binding protein (Cab) from Panax ginseng. The small subunit consists of 935 nucleotides long and has an open reading frame of 795 bp with the deduced amino acid of 265 residues (pI 5.63), 28.6 kDa. The deduced amino acid sequence matched to the previously reported Cab genes. Their degree of amino acid identity ranged from 68 to $92%$. Phylogenetic analysis based on the amino acid residues was showed that the ginseng Cab gene was grouped with P. persica (AAC34983), A. thaliana (AAD28771), G. hirsutum (CAA38025), G. max (AAL29886), and V. radiate (AAF89205).
 
Ű¿öµå
chlorophyll a/b binding proteins;Cab;leaf;Panax ginseng;
 
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸÁö / v.18, no.3, 2005³â, pp.441-449
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200507523330533)
¾ð¾î : Çѱ¹¾î
³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø
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