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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.19, no.2, 2006³â, pp.259-264
²¿¸®°í»ç¸®°ú(Aspleniaceae) ¾çÄ¡½Ä¹° 3Á¾ Àü¿±Ã¼ÀÇ ±â³»¹è¾ç¿¡ ¹ÌÄ¡´Â ¹èÁö±¸¼º¹°ÁúÀÇ ¿µÇâ
( Effect of Medium Composition on In vitro Prothallus Culture of 3 Fern Species in the Family Aspleniaceae )
Á¤Áø¾Æ;ÀÌöÈñ; ÃæºÏ´ëÇб³ ¿ø¿¹°úÇаú;ÃæºÏ´ëÇб³ ¿ø¿¹°úÇаú;
 
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º» ½ÇÇèÀº ²¿¸®°í»ç¸®°ú¿¡ ¼ÓÇÏ´Â 3Á¾ÀÇ ¾çÄ¡½Ä¹°µé(²¿¸®°í»ç¸®, °Å¹Ì°í»ç¸® ¹× º¯»êÀÏ¿±)¿¡¼­ ¹èÁöÀÇ Á¶¼º¹°Áú°ú ¹°¸®Àû Á¶°Ç ¹× »ýÀåÁ¶Àý¹°ÁúÀÌ Àü¿±Ã¼ »ýÀå¿¡ ¹ÌÄ¡´Â ¿µÇâÀ» ±¸¸íÇϱâ À§ÇÏ¿© ¼öÇàÇÏ¿´´Ù. 3Á¾ ¸ðµÎ¿¡¼­ Àü¿±Ã¼ ¹è¾ç¿¡ ÀûÇÕÇÑ ¹èÁö´Â MS¹èÁö·Î È®ÀεǾú´Ù. ¹èÁö ³» $NH_4^+:NO_3^-$ÀÇ ÀûÁ¤ ³óµµºñÀ²Àº ²¿¸®°í»ç¸®¿Í º¯»êÀÏ¿±ÀÇ °æ¿ì 20:40mM(1:2 ºñÀ²) À̾úÀ¸¸ç, °Å¹Ì°í»ç¸®´Â 30:30mM(1:1 ºñÀ²) À̾ú´Ù. Àü¿±Ã¼ »ýÀå¿¡ ÀûÇÕÇÑ ´ç ³óµµ´Â $1{sim}2%$¿´À¸¸ç, pH´Â $3.8{sim}5.8$À̾ú´Ù. ¹èÁö¿¡ ÷°¡µÈ agar ³óµµ´Â Àü¿±Ã¼ »ýÀå¿¡ º°´Ù¸¥ ¿µÇâÀ» ¹ÌÄ¡Áö ¾Ê´Â °ÍÀ¸·Î ³ªÅ¸³µ´Ù. 6Á¾ÀÇ »ýÀåÁ¶Àý¹°Áú(NAA, IA4, 2,4-D, BAP, kinetin ¹× 2ip)À» ¹èÁö¿¡ ÷°¡ÇÏ¿© 3Á¾ÀÇ Àü¿±À縦 ¹è¾çÇÑ °á°ú, ½ÃÅäŰ´Ñ·ùÀÇ °æ¿ì »ýÀåÃËÁøÈ¿°ú°¡ °üÂûµÇ¾úÀ¸³ª ¿Á½Å·ù´Â ³óµµ°¡ ³ô¾ÆÁú¼ö·Ï Àü¿±Ã¼ÀÇ »ýÀåÀ» ÀúÇØÇÏ´Â °ÍÀ¸·Î È®ÀεǾú´Ù.
This experiment was conducted to investigate the effects of medium composition, physical condition and growth regulators on prothallus proliferation in 3 fern species belonging to Aspleniaceae (Asplenium incisum, Camptosorus sibiricus and Phyllitis scolopendrium). In all of the 3 species, the effective medium for prothallus proliferation was Murashige and Skoog's basal medium with 1% sucrose and pH 5.8. The optimum $NH_4^+:NO_3^-$ concentration ratio of protahllus proliferation was 20:40mM (1:2 ratio) in A. incisum and P. scolopendrium and 30:30mM (1:1 ratio) in C. sibiricus. However, agar content of medium did not affect the rate of prothallus proliferation. Among the selected 6 kinds of growth regulators (NAA, IAA, 2,4-D, BAP, kinetin and lip), cytokinins generally has a promotive effect, but auxins showed an inhibitory effect on prothallus growth.
 
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Asplenium incisum;Camptosorus sibiricus;Phyllitis scolopendrium;Media composition;Proliferation;
 
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸÁö / v.19, no.2, 2006³â, pp.259-264
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200622219604684)
¾ð¾î : Çѱ¹¾î
³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø
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