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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.19, no.2, 2006³â, pp.308-314
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( Correlation between In vitro Plant Regeneration and Polarity with Boxthorn (Lycium chinense Mill.) Seedlings ) |
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| ±¸±âÀÚ³ª¹«·ÎºÎÅÍ Ä¶·¯½ºÀÇ Çü¼º°ú shootÀÇ Çü¼º¿¡ ¹ÌÄ¡´Â »ýÀåÁ¶ÀýÁ¦ÀÇ ¿µÇâÀ» ¿¬±¸ÇÑ °á°ú Ķ·¯½ºÀÇ Çü¼ºÀº 2,4-D¸¦ Æ÷ÇÔÇÏ´Â ¹èÁö¿¡¼ È¿°úÀûÀ̾úÀ¸³ª shootÀÇ Çü¼º¿¡´Â ¾ïÁ¦È¿°ú¸¦ º¸¿´´Ù. ShootÀÇ Çü¼ºÀº 0.1mg/L IAA + 0.1mg/L BAÀÇ ¹èÁö¿¡¼ Çü¼º·ü°ú ÀýÆí´ç Çü¼º °³Ã¼¼ö°¡ °¡Àå ³ôÀº ºÐÈÀ²À» º¸¿´´Ù. ÀýÆíüÀÇ ºÎÀ§¿¡ ±Ø¼º¿¡ ´Ù¸¥ Ķ·¯½ºÀÇ Çü¼º°ú shootÀÇ ÀçºÐÈ¿¡ À־ ÀÚ¿±ÀÇ ±âºÎ¿Í ¸»´ÜºÎ¸¦ 20% ÀýÁ¦ÇÑ ÀýÆíÀÇ ±âºÎÀÇ Àý´Ü¸é¿¡¼´Â shootÀÇ Çü¼ºÀÌ, ÇϺÎÀÇ Àý´Ü¸é¿¡¼´Â Ķ·¯½ºÀÇ Çü¼ºÀÌ ³ô¾ÒÀ¸¸ç ½Ã°£ÀÌ °æ°úÇÔ¿¡ µû¶ó ÇϺο¡¼µµ shootÀÇ Çü¼ºÀÌ º¸¿´´Ù. ÀçºÐȽŰÀº 1/3 MS ¹èÁö¿¡¼ »Ñ¸®°¡ ¾çÈ£ÇÏ°Ô ¹ß´ÞÇÏ¿´À¸¸ç Åä¾ç ¼øÈ°úÁ¤¿¡¼µµ 90% ÀÌ»óÀÇ »ýÁ¸À²À» º¸¿© °³È°¡ ÀÌ·ç¾îÁ³´Ù. |
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| This experiment was conducted out to investigate the effect of plant growth regulators on callus and shoot formation. The callus formation was effective on 1/2 MS medium containing 2,4-D, while shoot formation was suppressed. Shoot formation and differentiation were the highest in combination 0.1 mg/L of IAA and 0.1 mg/L of BA. The polarity of explants was investigated from cotyledon, which excised 20% of each basal and terminal parts. Formation of shoot was induced from excised ends of the basal part. In excised ends of the basal part, callus was induced vigorously and shoots were produced lately. Root induction was easily achieved in 1/3 MS medium from the adventitious shoot and more than 90% of regenerated plantlets acclimatized successfully and flowered normally. |
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| Polarity;Plantlet regeneration;Lycium chinense; |
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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸÁö / v.19, no.2, 2006³â, pp.308-314
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200622219605070)
¾ð¾î : Çѱ¹¾î |
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| ³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø |
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