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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.19, no.2, 2006³â, pp.336-339
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È«È ¼öÁýÁ¾ÀÇ RAPD¿¡ ÀÇÇÑ À¯¿¬°ü°è ºÐ¼®
( Intraspecific Relationship Analysis of Safflower (Carthamus tinctorius L.) Lines Collected by RAPD Markers ) |
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| È«È ¼öÁýÁ¾ÀÇ RAPD ºÐ¼®À» ÅëÇÑ À¯ÀüÀû ´Ù¾ç¼º ¹× À¯¿¬°ü°è¸¦ ¹àÈ÷°í ǰÁ¾±ºÀ» ºÐ·ùÇÏ¿© ǰÁ¾À°¼ºÀÇ ±âÃÊÀÚ·á·Î Ȱ¿ëÄÚÀÚ ½ÃÇèÇÑ °á°ú´Â ¾Æ·¡¿Í °°´Ù. RAPD ºÐ¼®¿¡ Àû¿ëÇÑ 37°³ÀÇ Primer Áß 25°³ÀÇ ÀûÁ¤ primer¸¦ ¼±¹ßÇÏ¿´°í, ÁõÆø¿ø PCR»ê¹°Àº $0.1{sim}4.0kb$¿¡¼ ÀçÇö¼ºÀÖ´Â band¸¦ º¸¿´À¸¸ç °¢ primer¿¡ ÀÇÇØ ÁõÆøµÈ bandÀÇ ¼ö´Â $1{sim}9$°³·Î ´Ù¾çÇÏ¿´°í Æò±Õ 4.8°³¿´´Ù. PCR ¹ÝÀÀ¿¡ »ç¿ëµÈ 25°³ÀÇ primer¿¡¼ 120°³ÀÇ band°¡ °üÂûµÇ¾úÀ¸¸ç ´ÙÇü¼ºÀ» º¸ÀÌ´Â bandµÉ ¼ö´Â 23°³(19.2%)¿´´Ù. RAPD-PCR¿¡ ÀÇÇØ ¾ò¾îÁø dendrogram¿¡¼ À¯¿¬°è¼ö 0.042¸¦ ±âÁØÀ¸·Î ÇÕÀ» ÇßÀ» ¶§ 6°³ ±ºÀ¸·Î ºÐ·ùµÇ¾ú°í II±º°ú III±ºÀº °¢°¢ 12Á¾(38%)¾¿ ¼ÓÇÏ¿´´Ù. |
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| This study was conducted to provide the genetic diversity on Safflower collections and to identify the variations which could be utilized in Safflower breeding. The RAPDs analysis was used to clarify the genetic relationships among 32 Safflower collections. Among 37 primers applied in RAPD analysis, 25 primers that generated appropriate PCR products for identification of the genetic characters in safflower collections were used. Amplified PCR showed the highly reproducible bands at $0.1{sim}4.0kb$. The number of bands amplified in each primer showed the variations ranging from 1 to 9, with the average of 5.6. A total of 25 bands were identified among twenty-five selected primers and 23 bands (19.2%) showed polymorphism. Based on the similarity value of 0.042 in dendrogram derived from the cluster analysis, the 32 Safflower collections were classified into 6 groups. The two main groups, II and III included 12 collections (38%) and 12 collections (38%), respectively. |
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| Ű¿öµå |
| Carthamus tinctorius L.;RAPD;Polymorphism; |
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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸÁö / v.19, no.2, 2006³â, pp.336-339
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200622219605324)
¾ð¾î : Çѱ¹¾î |
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| ³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø |
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