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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.19, no.2, 2006³â, pp.360-364
±â³»¹è¾ç¿¡¼­ µµ±úºñ°íºñ¿Í Âü¼è°íºñÀÇ Àü¿±Ã¼ ÇüÅÂÇü¼º
( Prothallus Morphogenesis of Cyrtomium falcatum (L.) Presl and Cyrtomium caryotideum var. coreanum Nakai In vitro Culture )
Á¤Áø¾Æ;ÀÌöÈñ; ÃæºÏ´ëÇб³ ¿ø¿¹°úÇаú, »ý¹°°Ç°­»ê¾÷°³¹ß¿¬±¸¼¾ÅÍ;ÃæºÏ´ëÇб³ ¿ø¿¹°úÇаú, »ý¹°°Ç°­»ê¾÷°³¹ß¿¬±¸¼¾ÅÍ;
 
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µµ±úºñ°íºñ¿Í Âü¼è°íºñÀÇ Æ÷Àڹ߾ƿ¡¼­ ¾òÀº Àü¿±Ã¼¸¦ ±ÕÁúÈ­ÇÏ¿© ¹è¾çÇÔÀ¸·Î½á Àü¿±Ã¼ °³Ã¼¹ß»ý°ú Æ÷ÀÚü Çü¼º°úÁ¤À» ¿¬±¸ÇÏ¿´´Ù. ¹è¾ç 2ÁÖÀÏÀÌ Áö³ªÀÚ ±ÕÁúÈ­ÇÑ Àü¿±Ã¼ ¼¼Æ÷µéÀÌ ÀÏÂ÷¿øÀÇ Çʶó¸àÆ® ÇüŸ¦ Çü¼ºÇÏ¿´°í, 4ÁÖÀϰ¿¡´Â ´Ù¼öÀÇ °¡Áö¸¦ »¸Àº ÇüÅÂÀÇ Àü¿±Ã¼°¡ ÃâÇöÇÏ¿´´Ù. ¹è¾ç 6ÁÖÀϰ¿¡ apical notch°¡ ¹ß´ÞµÈ Àü¿±Ã¼°¡ Çü¼ºµÇ¾ú´Âµ¥. Á߽ɺο¡´Â ºÐ¿­Á¶Á÷ÀÌ ¹ß´ÞµÇ¾ú´Ù. 8ÁÖÀÇ ¹è¾ç±â°£ÀÌ Áö³ªÀÚ Àü¿±Ã¼ÀÇ Á߽ɺο¡¼­ Àå¶õ±âÀÇ Çü¼º ¾øÀÌ ¹«¼öÁ¤»ý½Ä¿¡ ÀÇÇÑ bud°¡ °üÂûµÇ¾ú´Ù. 10ÁÖÀÏÀÌ Áö³ªÀÚ ¹«¼öÁ¤»ý½Ä¿¡ ÀÇÇÑ bud´Â Æ÷ÀÚü·Î ¹ß´ÞµÇ¾ú´Âµ¥, flow cytometric ºÐ¼® °á°ú µµ±úºñ°íºñ¿Í Âü¼è°íºñ ¸ðµÎ¿¡¼­ Àü¿±Ã¼¿Í Æ÷ÀÚü´Â µ¿ÀÏÇÑ ploidy levelÀ» Áö´Ñ °ÍÀ¸·Î È®ÀεǾú´Ù. ÀÌ´Â µµ±úºñ°íºñ»Ó¸¸ ¾Æ´Ï¶ó Âü¼è°íºñ ¶ÇÇÑ ¹«¼öÁ¤»ý½ÄÀ» ÇÏ´Â ¾çÄ¡½Ä¹°ÀÓÀ» Áõ¸íÇÏ´Â °á°ú·Î »ý°¢µÈ´Ù.
The gametophytes of Cyrtomium falcatum and Cyrtomium caryoptideum var. coreanum arising from spores were mechanically homogenized and cultured In vitro, to study their gametophyte ontogeny and sporophyte development. Homogenized gametophytic tissues formed as one-dimensional filaments after 2 weeks in culture and then glowed into blanched gamatophytes after 4 weeks. After 6 weeks, which were developed to two dimensional plates with apical notch and meristem in central zone. After 8 weeks in culture, apomictic buds were formed on the midribs without archegonium formation and these buds developed to sporophytes after 10 weeks in culture. Flow cytometric analysis of gametophytes and apomictic sporophytes revealed that both forms had the same ploidy level in C. falcatum and C. caryoptideum vu. coreanum, respectively. This is to certify that C. caryoptideum var. coreanum was an apomictic fern as well as C. falcatum.
 
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Gametophyte ontogeny;Homogenized;Apomictic fern;
 
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸÁö / v.19, no.2, 2006³â, pp.360-364
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200622219605649)
¾ð¾î : Çѱ¹¾î
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