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한국자원식물학회 / v.19, no.3, 2006년, pp.422-426 
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( Protoplast Culture by Electrofusion of Protoplasts from Solanum sisymbriifolium and Other Solanum Species ) |
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| 초 록 |
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| This research was conducted to get the basic materials necessary to obtain the somatic hybrid plant between Solanum sisymbriifolium and other Solanum species (S. integrifolium and S. toxicarium). Regarding the formation of colony from the protoplast in S. sisymbriifolium, S. integrifolium and the fused protoplast mixture; for the S. sisymbriifolium, a colony was observed in F medium(Kao medium containing $5.0mg{cdot}L^{-1};NAA,;1.0mg{cdot}L^{-1}$ 2,4-D and $1.0mg{cdot}L^{-1}$ BA); and for the S. integrifolium, in G medium (a half strength MS medium containing 0.03 M sucrose, 0.4 M mannitol, $1.0mg{cdot}L^{-1};NAA,;1.0mg{cdot}L^{-1}$ kinetin) respectively. In mixed cultured protoplast after electriofusion treatment, the cell division and colony formation were observed in both media F and G. For the shoot and root formation rate, there was no difference between the parent of each breed and mixed protoplast regardless of the medium. In the fused protoplast mixture of S. sisymbriifolium and S. toxicarium, a colony formation was also observed in both media F and H(a half strength MS medium containing 0.03 M sucrose, 0.4 M mannitol, $1.0mg{cdot}L^{-1};NAA,;1.0mg{cdot}L^{-1}$ kinetin); and there was no difference in the shoot and root formation rate between the parent and the mixed protoplast. |
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| 키워드 |
| Solanum species;Culture media;Isozyme pattern; |
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한국자원식물학회지 / v.19, no.3, 2006년, pp.422-426
한국자원식물학회
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200631037008093)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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