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한국자원식물학회 / v.19, no.3, 2006년, pp.440-446 
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( Isolation of Differentially Expressed Genes by Low Temperature Treatment in Winter Oilseed Rape (Brassica napus L. cv. Tammi) ) |
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| 초 록 |
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| To investigate flowering related genes in winter-type oilseed rape (Brassica napus L. cv. Tammi), differentially expressed genes were isolated from leaves of the plant after low temperature treatment which is requirements for floral induction. As a result of suppression subtractive hybridization (SSH), 288 clones were randomly selected from SSH library. Using reverse Northern blot analysis, 150 of 288 clones were identified to be differentially expressed. Out of these 150 clones, 45 clones showed very high identities with the known genes. Four clones showed very high identities over 90% with metallothionein-like gene that is related to flowering-induced genes. Of these 4 clones, the cDNA clone, rfs-13, revealed high identity with meotallothionein-like protein in Arabidopsis thaliana (98%) and Brassica compestris (89%). Furthermore, gene expressed in immature flower stages was confirmed by Northern blot analysis. |
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| 키워드 |
| Brassica napus;cDNA;Flowering;Oil seed rape;Suppression subtractive hybridization (SSH);Transcription; |
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한국자원식물학회지 / v.19, no.3, 2006년, pp.440-446
한국자원식물학회
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200631037008195)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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