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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.19, no.4, 2006³â, pp.491-496
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Staphylococcus aureus¿¡ ´ëÇÑ ÇѾàÀçÀÇ Ç×±ÕȰ¼º
( Antimicrobial Activity of Medicinal Herbs against Staphylococcus aureus ) |
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| 18Á¾ÀÇ ÇѾàÀç MeOH ÃßÃâ¹°°ú EtOH ÃßÃâ¹°ÀÇ Staphylococcus aureus¿¡ ´ëÇÑ Ç×±Õ·ÂÀ» Á¶»çÇÑ °á°ú ÇϰíÃÊ, ¼Ò¸ñ, ¿À¹èÀÚ ¹× ÇâÀ¯´Â 5mg/ml ¼öÁØ¿¡¼ Ç×±ÕȰ¼ºÀ» ³ªÅ¸³»¾úÀ¸¸ç Áö½Ç, ±ÝÀºÈ ¹× Ȳ±Ý µîÀº 10mg/ml, ¿À¹ÌÀÚ, Ãʵα¸ ¹× Èñ·Å µîÀº 30mg/ml À̻󿡼 Ç×±Õ¼ºÀ» º¸¿´´Ù. ÃÖ¼ÒÀúÇØ³óµµ´Â MeOHÃßÃâ¹°¿¡¼´Â $1{sim}34mg/ml$, EtOHÃßÃâ¹°¿¡¼´Â $1{sim}46mg/ml$ ³óµµ¿¡¼ Ç×±ÕȰ¼ºÀ» º¸¿´À¸¸ç ºÐȹ Ãþ Áß EtOAcÃþ¿¡¼ ¼ºÀå¾ïÁ¦È¿°ú°¡ °¡Àå ³ô°Ô ³ªÅ¸³µ´Ù. Ç×±Õ·ÂÀÌ °¡Àå ¿ì¼öÇÑ °ÍÀ¸·Î È®ÀÎµÈ ¼Ò¸ñ, ¿À¹èÀÚ ¹× Ȳ±ÝÃßÃâ¹°ÀÇ ¹Ì»ý¹° Áõ½Ä¾ïÁ¦ È¿°ú¸¦ Á¶»çÇϱâ À§ÇØ Áõ½Ä¹èÁö¿¡ °¢°¢ÀÇ ÃßÃâ¹°À» 0, 100, 300 ¹× 500ppmÀÇ ³óµµ·Î ÷°¡ÇÏ¿© ±ÕÁÖÀÇ Áõ½ÄÀ» Á¶»çÇÑ °á°ú ¹è¾çÈÄ 24½Ã°£¿¡ ¼Ò¸ñ ÃßÃâ¹° ¹«Ã·°¡±¸ÀÇ $OD_{620}$°ªÀÌ 0.4ÀÎ ¹Ý¸é 100ppm ÀÌ»óÀÇ ÃßÃâ¹° ÷°¡ ½Ã $0.02{sim}0.03$Á¤µµ·Î ±ÕÁõ½ÄÀÌ ÇöÀúÈ÷ ¾ïÁ¦µÇ¾ú°í, ¿À¹èÀÚ ÃßÃâ¹° ¹«Ã·°¡±¸ÀÇ $OD_{620}$°ªÀÌ 0.4ÀÎ ¹Ý¸é 300ppm ÀÌ»óÀÇ ÃßÃâ¹° ÷°¡ ½Ã $0.02{sim}0.03$Á¤µµ·Î ±ÕÁõ½ÄÀÌ ÇöÀúÈ÷ ¾ïÁ¦µÇ¾úÀ¸¸ç Ȳ±Ý ÃßÃâ¹° ¹«Ã·°¡±¸ÀÇ $OD_{620}$°ªÀÌ 0.4ÀÎ ¹Ý¸é 300ppm ÀÌ»óÀÇ ÃßÃâ¹° ÷°¡ ½Ã $0.02{sim}0.07$ Á¤µµ·Î ±ÕÁõ½ÄÀÌ ÇöÀúÈ÷ ¾ïÁ¦µÇ¾ú´Ù. ÀÌ»óÀÇ °á°ú·Î º¼ ¶§ Á¦´ë¿°, ºÎÁ¾¼º ÇǺο° ¹× °üÀý¿° µîÀÇ ´Ù¾çÇÑ °¡ÃàÁúº´À» ÀÏÀ¸Å°´Â Staphylococcus aureusiÀÇ °¨¿°À» ¿¹¹æÇϰųª ¶Ç´Â Ä¡·áÇÒ ¼ö ÀÖ´Â ÇѾàÀç ÃßÃâ¹° »ç·á÷°¡Á¦ÀÇ °³¹ß °¡´É¼ºÀ» È®ÀÎ ÇÒ ¼ö ÀÖ¾ú´Ù. |
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| Antimicrobial activity of 18 different traditional medicinal herbs extracts against Staphylococcus aureus was determined by a paper disc method. The Prunella vulgaris, Caesalpinia sappan and Rhus javanica extracts in 5 mg/ml, Poncirus trifoliata, Lonicera japonica and Seutellaria baicalensis extracts in 10 mg/ml and Schizandra chinensis, Alpinia katsumadai, Siegesbeckia orientalis extracts in 30 mg/ml showed a significant antimicrobial activity against Staphylococcus aureus. Minimum inhibitory concentrations of medicinal herbs extracts were in the range of $1{sim}34;mg/ml$ and $1{sim}46;mg/ml$, in the case of MeOH extracts and EtOH extracts, respectively. In addition, the antimicrobial activity of each solvent fraction was most significant with EtOAc layer. Optical density at 620nm after 24 hours incubation of Staphylococcus aureus in the presence of 100, 300 or 500 ppm of Caesalpinia sappan extract ranged from 0.02 to 0.03 compared to 0.4 in the absence of Caesalpinia sappan extract, indicating that growth of Staphylococcus aureus was significantly inhibited within 24 hours by the addition of at least 100 ppm of Caesalpinia sappan extract. Optical density at 620 nm after 24 hours incubation of Staphylococcus aureus in the presence of 300 ppm of Rhus javanica extract ranged from 0.02 to 0.03 compared to 0.4 in the absence of Rhus javanica extract, indicating that growth of Staphylococcus aureus was also significantly inhibited within 24 hours by the addition of at least 300 ppm of Rhus javanica extract. Optical density at 620 nm after 24 hours incubation of Staphylococcus aureus in the presence of 300 ppm of Seutellaria baicalensis extract ranged from 0.02 to 0.07 compared to 0.4 in the absence of Seutellaria baicalensis extract, indicating that growth of Staphylococcus aureus was also significantly inhibited within 24 hours by the addition of at least 300 ppm of Seutellaria baicalensis extract. In conclusion, these findings suggest that extracts from medicinal herbs may play important roles for antimicrobial activities against Staphylococcus aureus. |
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| Ű¿öµå |
| Antimicrobial Activity;Medicinal Herb;Staphylococcus aureus; |
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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸÁö / v.19, no.4, 2006³â, pp.491-496
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200603043151566)
¾ð¾î : Çѱ¹¾î |
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| ³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø |
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