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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.19, no.4, 2006³â, pp.524-529
Àڱͳª¹«(Albizzia julibrissin Duraz.)ÀÇ ±â³» ´ë·®Áõ½Ä
( In Vitro Shoot Multiplication of Albizzia julibrissin Duraz. )
¾ÈÁö¿µ;±èÁ¤Èñ;°­È£´ö; µ¿±¹´ëÇб³ »ý¸íÀÚ¿ø°úÇдëÇÐ »ê¸²ÀÚ¿øÇаú;µ¿±¹´ëÇб³ »ý¸íÀÚ¿ø°úÇдëÇÐ »ê¸²ÀÚ¿øÇаú;µ¿±¹´ëÇб³ »ý¸íÀÚ¿ø°úÇдëÇÐ »ê¸²ÀÚ¿øÇаú;
 
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Àڱͳª¹«ÀÇ ´ë·®Áõ½ÄÀ» À§ÇÑ ½ÅÃÊ Çü¼º ¹× »Ñ¸® À¯µµ¸¦ À§ÇØ ½ÃÅäŰ´Ñ°ú ¿Á½ÅÀÌ ÇÔÀ¯µÈ ¹èÁö¿¡ ¹è¾çÇÏ¿´´Ù. ÀüüÀûÀÎ ½ÅÃÊ À¯µµÀ²Àº Æò±Õ 56.2%¿´À¸¸ç, ±× Áß °¡Àå ³ôÀº À¯µµÀ²À» º¸ÀÎ °ÍÀº 73%ÀÇ zeatinÀ¸·Î ½ÅÃÊ Çü¼º¿¡ È¿°úÀûÀÎ °ÍÀ¸·Î ³ªÅ¸³µÀ» »Ó ¾Æ´Ï¶ó TDZ¿Í È¥¿ë ó¸®ÇßÀ» ¶§¿¡µµ ¶Ù¾î³µ´Ù. ƯÈ÷, zeatin 1.0mg/L¸¦ ó¸®ÇÑ ÇϹèÃà¿¡¼­ ´ë·®ÀÇ ÁٱⰡ Çü¼ºµÇ¾úÀ¸¸ç, È¥¿ëó¸®ÀÇ °æ¿ì zeatin 2.0 + TDZ 0.05mg/LÀÇ »Ñ¸® ÀýÆí¿¡¼­ È¿°úÀûÀ¸·Î ³ªÅ¸³µ´Ù. Áõ½ÄµÈ ½Ä¹°Ã¼ÀÇ »Ñ¸® À¯µµ´Â ½Ä¹°»ýÀåÁ¶Àý¹°Áú 󸮺¸´Ù´Â ¹«Ã³¸® ¹èÁö¿¡¼­ Ȱ¹ßÇÑ °ÍÀ¸·Î ³ªÅ¸³µ´Ù. º» ¿¬±¸´Â ±â³» Á¶Á÷¹è¾çÀ» ÅëÇØ Àڱͳª¹« ½Ä¹°Ã¼¿¡¼­ ½ÅÃʸ¦ ´ë·®À¸·Î Çü¼º½ÃÄ×°í, Áõ½ÄµÈ ½ÅÃÊ¿¡¼­ ´Ù½Ã »Ñ¸®¸¦ À¯µµÇÔÀ¸·Î½á ¿Â½Ç¿¡¼­ °æÈ­½Ãų ¼ö ÀÖ¾ú´Ù. ÀÌ·Î½á ¾à¿ë¼öÁ¾ÀÎ Àڱͳª¹«ÀÇ ´ë·® Áõ½ÄÀÌ °¡´ÉÇÑ Åä´ë¸¦ ¸¶·ÃÇÏ¿´°í, ¾à¿ë¼ö¸ñÀÚ¿ø¿¡ ´ëÇÏ¿© Á¶Á÷¹è¾çÀ» ÀÌ¿ëÇÏ¿© ±â³»¿¡¼­ Àå±âÀûÀ¸·Î º¸Á¸ÇÒ ¼ö ÀÖ´Â ±â¹ÝÀ» ¸¶·ÃÇϴµ¥ ÀÇÀǰ¡ ÀÖ´Ù°í ÇÒ ¼ö ÀÖ´Ù.
In vitro culture system was established to induce multiple shoots of Albizzia julibrissin Duraz. by investigating the effects of cytokinins. Cotyledon, hypocotyl and root explants were cultured on MS media supplemented with either three different plant growth regulators or their combinations. The most effective cytokinin sources were zeatin 2.0 + TDZ 0.5 mg/L in cotyledon, zeatin 1.0 mg/L in hypocotyl, and BA 0.2 + TDZ 0.01 mg/L in root explant for producing shoots ($5.67;{pm};1.20$, $19.50;{pm};3.50$, and $20.50;{pm};2.47$, respectively). Also, zeatin treatment was tended to induce more shoots rather than the combinations of other cytokinins. In addition, the root induced in 1/2 MS medium without any plant growth regulators was longer and thicker than treatments of IBA, NAA, IAA and 2.4-D as auxins. Overall, the highest average percent of in vitro shoot formation was 73% from three different types of explants with treatment of zeatin (1.0mg/L).
 
Ű¿öµå
Albizzia julibrissin;Cotyledon;Hypocotyl;Plant growth regulators (PGRs);Root explant;Shoot multiplication;
 
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸÁö / v.19, no.4, 2006³â, pp.524-529
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200603043151643)
¾ð¾î : Çѱ¹¾î
³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø
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