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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.20, no.4, 2007³â, pp.272-280
»ï¹éÃÊ¿Í ½Ä¹° 2Á¾ÀÇ Áö¿ª°³Ã¼±ºº° RAPD ºÐ¼®
( RAPD Analyses on the Regional Populations of Two Species of Saururaceae in Korea )
ŰæÈ¯;±è¿ëÇö;µµÀçÈ­;±èÁÖȯ; ´ëÀü´ëÇб³ »ý¸í°úÇаú;´ëÀü´ëÇб³ »ý¸í°úÇаú;´ëÀü´ëÇб³ »ý¸í°úÇаú;´ëÀü´ëÇб³ »ý¸í°úÇаú;
 
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»ï¹éÃʰú½Ä¹° 2¼Ó 2Á¾¿¡ ´ëÇÑ 30°³ Áö¿ªº° °³Ã¼±ºÀÇ À¯ÀüÀû »ó°ü°ü°è¸¦ ¾Ë¾Æº¸±â À§ÇØ RAPD ºÐ¼®À» ½Ç½ÃÇÏ¿´´Ù. PCR °úÁ¤À» ÅëÇØ ÁõÆøµÈ RAPD ÀýÆíµéÀº 300bp¿¡¼­ 2,000bp »çÀÌÀÇ ±¸°£¿¡¼­ °üÂûµÇ¾ú´Ù. ÃÑ 16°³ÀÇ oligoprimer¸¦ ÀÌ¿ëÇÑ È¿¼ÒÁßÇÕ¹ÝÀÀ¿¡¼­ 156°³ÀÇ À¯È¿ÇÑ polymorphic band makers¸¦ È®ÀÎÇÏ¿´°í ÀÌ·¯ÇÑ ÀÚ·á¿¡ ±Ù°ÅÇÏ¿© 2Á¾ÀÇ Á¾³» °³Ã¼±º ±ºÁý¿¡ ´ëÇÑ UPGMA phenogramÀ» µµÃâÇÏ¿´´Ù. ÀÌ °á°ú À¯ÁýÇüÅ¿¡ ±Ù°ÅÇÏ¿© Àç¹èÁö °³Ã¼±º°ú ÀÚ¿¬Áý´Ü °³Ã¼±º°úÀÇ ±¸ºÐÀÌ °¡´ÉÇÏ¿´°í ¶ÇÇÑ Áö¿ªº° °³Ã¼±ºµé³¢¸® À¯ÁýµÇ´Â °á°ú¸¦ º¸¿´´Ù. 2Á¾ ¸ðµÎ¿¡¼­ Áö¿ªº° °³Ã¼±ºÁß °æ³²ÀÇ °³Ã¼±º°ú Á¦ÁÖµµÀÇ °³Ã¼±ºÀÌ Àü³²ÀÇ °³Ã¼±º¿¡ ºñÇØ À¯¿¬°ü°è°¡ ¹ÐÁ¢ÇÑ °ÍÀ¸·Î ¹àÇôÁ³´Ù. µû¶ó¼­ RAPD ºÐ¼®Àº »ï¹éÃʰú ½Ä¹°ÀÇ °³Ã¼±ºº° À¯¿¬°ü°è¸¦ ºÐ¼®Çϰųª Àç¹èÁý´Ü°ú ÀÚ¿¬Áý´ÜÀ» ºÐ¼®Çϴµ¥ À¯¿ëÇÑ ½ÇÇèÀû ¹æ¹ýÀ¸·Î »ý°¢µÈ´Ù.
In order to presume the genetic relationship about two species and their 30 regional populations of the Saururus and Houttuynia of Saururaceae, RAPD analyses were performed. The length of amplified DNA fragments ranged from 300 to 2,000 bp. 156 scorable RAPD makers were found from PCR reactions with sixteen random oligoprimers. Also, some regional populations were clustered separately from the UPGMA phenogram. The OTUs between cultivated and natural populations were distinguished distinctly on the UPGMA phenogram. And the regionals populations of the treated taxa were clustered. Among the regional populations of two species, GN populations had the close relationship JJ populations rather than JN populations. The RAPD data was very useful to define the genetic relationship and distinguish the cultivated populations from natural populations by regional distributions in this study.
 
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Genetic relationship;Saururus;Houttuynia;Saururaceae;Populations;
 
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸÁö / v.20, no.4, 2007³â, pp.272-280
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200736038131437)
¾ð¾î : Çѱ¹¾î
³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø
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