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한국자원식물학회 / v.19, no.6, 2006년, pp.665-669 
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( Cryopreservation of Somatic Embryos of Soapbeny (Sapindus mukorossi Gaertn.) by Vitrification ) |
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| 초 록 |
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| Somatic embryos do not survive at exposure to liquid nitrogen temperatures without cryoprotective treatments. A simplified technique which simultaneously induces and cryoprotects embryogenic calli using plant vitrification solution 2 (PVS2) followed by dehydration was developed for the cryopreservation of Soap berry genetic resources. Vitrification is a way of removing the moisture in vegetation through PVS2. The PVS2 vitrification solution consisted of 30% glycerol (w/v), 15% ethylene glycol (w/v), 15% Dimethylsulfoxide (w/v) in B5 medium containing 0.4M sucrose. Two tests were done. The one was to eliminate moisture at $0^{circ}C$ and the other at $25^{circ}C$. In both cases the best results came out at a vitrification time of $10{sim}20$ minutes. It was also found that the survival rate was higher at $0^{circ}C$ than at $25^{circ}C$. In particular, the survival rate reached more than 80%. Water-damaged embryos turned brown and stoped growth, but energetic embryos took on a milky hue and show a very vigorous growth rate. Successful cryopreservation of somatic embryos of soapberry can be used to establish in vitro genebanks for long-term conservation of Soapberry genetic resources to complement field genebanks and other in vitro methods already being used. |
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| 키워드 |
| Cryopreservation;Somatic embryo;Sapindus mukorossi;Vitrification; |
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한국자원식물학회지 / v.19, no.6, 2006년, pp.665-669
한국자원식물학회
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200606141806492)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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