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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.21, no.4, 2008³â, pp.260-264
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°íÃß À¯ÀüÀÚº¯Çüü ÈÄ´ë »ýÀ°Æ¯¼º °ËÁ¤
( Characteristic of Progeny in Pepper Transformants ) |
| ±ÇÅ·æ;À̹®Áß;ÇÑÁõ¼ú;½Åµ¿Çö;¿ÀÁß¿;±è°æ¹Î;±èâ±æ; °æºÏ³ó¾÷±â¼ú¿ø;°æºÏ³ó¾÷±â¼ú¿ø;¿ø¿¹¿¬±¸¼Ò;°æºÏ´ëÇб³ ½Ä¹°»ý¸í°úÇкÎ;°æºÏ´ëÇб³ ȯ°æ¿ø¿¹Çаú;°æºÏ´ëÇб³ ȯ°æ¿ø¿¹Çаú;°æºÏ´ëÇб³ ȯ°æ¿ø¿¹Çаú;
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| ÃÊ ·Ï |
| °íÃß ÇüÁúÀüȯü $T_1$ ¹× $T_2$¼¼´ëÀÇ À¯¹¦¿¡ ¿ªº´±ÕÀ» Á¢Á¾ÇÑ °á°ú À¯ÁÖÆ÷ÀÚ $10^3$°³/mL¿¡¼ Á¢Á¾ 5ÀÏÈÄ ¿ªº´ ¹ßº´À²Àº $T_1-1$°èÅë 4.0%, $T_1-2$ 10.0%À̾úÀ¸¸ç, Á¢Á¾ 12ÀÏÈÄ´Â $T_1-1$ °èÅë 52.0%, $T_1-2$ °èÅë 64.0%·Î ´ëºñÁ¾ÀÎ '±Ýž'°ú '¼öºñÃÊ' 100%¿¡ ºñÇØ ¹ßº´À²ÀÌ ¶³¾îÁ® À¯ÁÖÆ÷ÀÚ $10^3$°³/mL¿¡¼´Â ÇüÁúÀüȯüÀÇ ÀúÇ×¼º °èÅë¼±¹ßÀÌ °¡´ÉÇÏ¿´´Ù. ȹµæµÈ ¿ªº´ ÀúÇ×¼º ÇüÁúÀüȯü¸¦ ¿µ¾ç°íÃß½ÃÇèÀåÀÇ ¿ªº´ »ó½ÀÆ÷Àå¿¡¼ Àç¹èÇÑ °á°ú »ýÀ°, °³È¼Ò¿äÀϼö, °úƯ¼º µîÀº °èÅë°£¿¡ ´Ù¼Ò Â÷À̰¡ ÀÖ¾úÀ¸³ª, °ú½Ç¸ð¾çÀº ¼öºñÃÊ¿Í ¸Å¿ì À¯»çÇÏ¿´´Ù. Æ÷Àå¿¡¼ÀÇ ¿ªº´ÀÇ ¹ß»ýÀº °èÅë°£¿¡ Â÷À̰¡ ¸¹¾ÒÀ¸¸ç, $T_2-1-6$ °èÅë°ú $T_2-4-9$ °èÅëÀº ½ÃÆÇÁ¾ÀÎ ±Ýž¿¡ ºñÇØ ¾à 30%Á¤µµ Àû¾ú´Ù. Æ÷Àå¿¡¼ ¼±¹ßµÈ ÇüÁúÀüȯü´Â ¼¼´ëÀÇ ÁøÀüÀ» ÅëÇÑ ÀúÇ×¼ºÀÇ °íÁ¤ÀÌ ÇÊ¿äÇÑ °ÍÀ¸·Î ÆÇ´ÜµÈ´Ù. ÀÌ»óÀÇ °á°ú·Î º» ¿¬±¸¿¡¼ È®¸³µÈ ÇüÁúÀüȯ ½Ã½ºÅÛÀ¸·Î °íÃßÀÇ ¿ªº´ÀúÇ×¼º ÇüÁúÀüȯüÀÇ À°¼ºÀÌ °¡´ÉÇÒ °ÍÀ¸·Î »ý°¢µÈ´Ù. |
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| For the resistance test for Phytophthora blight of $T_1$ and $T_2$ transformants in pepper, Phytophthora blight fungus was inoculated to seedlings of the $T_1$ and $T_2$ transformants by concentration (density: zoospore $10^3/ml$). Occurrence rate of blight at 5days after inoculation was 4.0 % in T1-1 line and 10.0% in $T_1-2$ line, and its rate for 12 days after inoculation was 52.0% in $T_1-1$ line, 64.0% in $T_1-2$ line, respectively. Therefore, the lower occurrence rate to blight was enable to select resistant transformants in the some inoculation density (zoospore $10^3/ml$), meanwhile 'Kumtap' and 'Subicho' were 100% in highest occurrence rate to blight. For field test, in which blight was commonly occurred, of the Youngyang Pepper Experiment Station, the acquired transformant resisting to blight was similar to characteristics of domestic varieties, 'Subic
ho' for fruit shape, but there are some differences in growth, days to flowering, fruit characteristics. Occurrence of blight in $T_2-1-6$, and $T_2-4-9$ lines was smaller approxmately 30% than commercial varieties, 'Kumtap', although occurrence of blight in field was showed higher difference among tested lines. In this study, we concluded that the transformants showing blight resistance selected from habitual field could be fixed at every generation, and the developed transformation system was also considered to develop transformants in pepper. |
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| Ű¿öµå |
| Red pepper;Phytophthora blight;Transformation;Disease resistance;Field test; |
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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸÁö / v.21, no.4, 2008³â, pp.260-264
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200831050534485)
¾ð¾î : Çѱ¹¾î |
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| ³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø |
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