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한국자원식물학회 / v.21, no.6, 2008년, pp.449-456 
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( Buddleja officinalis prevents the normal cells from oxidative damage via antioxidant activity ) |
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| 초 록 |
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| The flowers of Buddleja officinalis are used to treat sore and damaged eyes, a condition which is similar to skin wounds. However, whether it has any protective effect on oxidative DNA damage and cell death induced by hydroxyl radical remains unclear. In this study, we evaluated the protective effects of the extracts against oxidative DNA and cell damage caused by hydroxyl radical. DPPH radical, hydroxyl radical, hydrogen peroxide and intracellular ROS scavenging assay, and $Fe^{2+}$ chelating assay were used to evaluate the antioxidant properties. phi X 174 RF I plasmid DNA and intracellular DNA migration assay were used to evaluate the protective effect against oxidative DNA damage. Lastly, MTT assay and lipid peroxidation assay were used to evaluate the protective effect against oxidative cell damage. It was found to prevent intracellular DNA and the normal cells from oxidative damage caused by hydroxyl radical via antioxidant activities. These results suggest that Buddleja officinalis may exert the inhibitory effect on ROS-induced carcinogenesis by blocking oxidative DNA damage and cell death. |
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| 키워드 |
| Reactive Oxygen Species (ROS);Oxidative DNA damage;Oxidative Cell Death;Carcinogenesis;Lipid peroxidation; |
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한국자원식물학회지 / v.21, no.6, 2008년, pp.449-456
한국자원식물학회
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200805441027419)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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