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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.19, no.1, 2006³â, pp.174-179
°í·ÁÀλï(Panax ginseng C.A, Meyer)ÀÇ ÀÙ ESTs database¿¡¼­ Energy ´ë»ç °ü·Ã À¯ÀüÀÚ ºÐ¼®
( Gene Analysis Related Energy Metabolism of Leaf Expressed Sequence Tags Database of Korean Ginseng (Panax ginseng C.A. Meyer) )
ÀÌÁ¾ÀÏ;À±ÀçÈ£;¼Û¿ø¼·;À̹ü¼ö;ÀÎÁر³;±èÀºÁ¤;¾ç´öÃá; ¼øÃµ´ëÇб³ ÀÚ¿¬°úÇдëÇÐ;¼øÃµ´ëÇб³ ³ó¾÷»ý¸í°úÇдëÇÐ;¼øÃµ´ëÇб³ ³ó¾÷»ý¸í°úÇдëÇÐ;(ÁÖ)¹ÙÀÌ¿ÀÇǾÆ;(ÁÖ)¹ÙÀÌ¿ÀÇǾÆ;(ÁÖ)¹ÙÀÌ¿ÀÇǾÆ;°æÈñ´ëÇб³ ÇѹæÀç·á°¡°øÇаú;
 
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º» ¿¬±¸¿¡¼­´Â Àλï ÀÙÀ¸·ÎºÎÅÍ Á¤Á¦ÇÑ mRNA¸¦ ÀÌ¿ëÇÏ¿© cDNA library¸¦ Á¦ÀÛÇÏ¿´´Ù. ÀÌ cDNA library·Î ºÎÅÍ 349°³ÀÇ ¿¡³ÊÁö ´ë»ç °ü·Ã À¯ÀüÀÚ¸¦ ¼±¹ß ÇÏ¿´´Ù. ¿¡³ÊÁö ´ë»ç °ü·Ã À¯ÀüÀÚÀÇ Æò±Õ »çÀÌÁî´Â 0.49 kbÀ̸ç, ¿¡³ÊÁö °ü·Ã À¯ÀüÀÚµéÀÇ ¼¼ºÎ ±â´Éº° ¹ßÇöÀ» ºÐ¼®ÇÑ °á°ú aerobic respiration(48.4%), accessory proteins of electron transport and membrane associated energy conservation(17.2%), glycolysis and gluconeogenesis(3.4%), electron transport and membrane associated energy conservation(2.9%), respiration(2.0%), glycolysis methylglyoxal byp-ass(1.7%), metabolism of energy reserves(0.6%)¿Í alcohol fermentation(0.3%)ÀÇ ºÐÆ÷¸¦ º¸¿´´Ù. Àλï ÀÙ¿¡¼­ ¹ßÇöµÇ´Â À¯ÀüÀÚÁß °¡Àå ¸¹ÀÌ ¹ßÇöµÈ Chlorophyll a/b binding protein of IhcII type I(36.6%), Photosystem II oxygen-evolving complex protein(6.6%) µîÀÌ ¹ßÇöµÇ¾ú´Ù.
A cDNA library was constructed from leaf samples of 4-year-old Panax ginseng cultured in a field. 3,000 EST from a size selected leaf cDNA library were analyzed. The 349 of 2,896 cDNA clones has related with energy metabolism genes. The 349 known genes were categorized into nine groups according to their functional classification, aerobic respiration(48.4%), accessory proteins of electron transport and membrane associated energy conservation(17.2%), glycolysis and gluconeogenesis(3.4%), electron transport and membrane associated energy conservation(2.9%), respiration(2.0%), glycolysis methylglyoxal bypass(1.7%), metabolism of energy reserves(0.6%) and alcohol fermentation(0.3%).
 
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ESTs database;Metabolism;Leaf;Energy;Panax ginseng;
 
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸÁö / v.19, no.1, 2006³â, pp.174-179
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200617033413066)
¾ð¾î : Çѱ¹¾î
³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø
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