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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.18, no.1, 2005³â, pp.154-160
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( Effect of Plant Growth Regulators on in vitro Micropropagation of Colored Calla Lily(Zantedeschia spp.) ) |
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| À¯»ö Ä®¶ó ¼¼ ǰÁ¾(Sunlight, Chiante, Pink Persuation)ÀÇ ±â³» ±Þ¼ÓÁõ½Äü°è¸¦ È®¸³ÄÚÀÚ Á¤´ÜºÐ ¿Á¶Á÷À» ¹è¾çÇÔ¿¡ ÀÖ¾î callus, ½ÅÃÊ ¹× »Ñ¸® ºÐÈ¿¡ ¹ÌÄ¡´Â ½Ä¹°»ýÀåÁ¶Àý¹°ÁúÀÇ È¿°ú¿Í sucrose°¡ ±â³» ±«°æ Çü¼º ¹× ºñ´ë¿¡ ¹ÌÄ¡´Â È¿°ú¸¦ Á¶»çÇÏ¿´´Ù. ½Ä¹°Ã¼´Â Ķ·¯½º¸¦ ÅëÇϰųª ¶Ç´Â Ä¡»óü¿¡¼ Á÷Á¢ ºÐȵǴ µÎ °æ·Î·Î Çü¼ºµÇ¾úÀ¸¸ç 2.0 mg/L BA ´Ü¿ë 󸮿¡¼ SunlightǰÁ¾Àº $53.3%$ Ķ·¯½º¸¦, Chiante ǰÁ¾Àº $56.7%$ Á÷Á¢ ½ÅÃʸ¦ Çü¼ºÇÏ¿´´Ù. 2.0-3.0 mg/L BA ´Ü¿ë 󸮴 ǰÁ¾º°·Î ½Ä¹°Ã¼Àç ºÐÈ ºóµµ°¡ $20-70%$·Î Â÷À̰¡ ÀÖ¾úÀ¸³ª ¼¼ ǰÁ¾ ¸ðµÎ 3.0 mg/L. BA ´Ü¿ë 󸮿¡¼ ½ÅÃÊ ºÐȰ¡ ¾çÈ£ÇÏ¿´´Ù. ´Ù¾ÆÃ¼ Çü¼º¿¡ ¹ÌÄ¡´Â cytokininÀÇ È¿°ú´Â SunlightǰÁ¾Àº 1.0 mg/L 2ip 󸮷Π16°³Ã¼¸¦, Chiante ǰÁ¾Àº 5.0 mg/L BA¸¦ 󸮷Π14°³Ã¼¸¦, Pink PersuasionǰÁ¾Àº 1.0 mg/L BA󸮷Π12°³Ã¼¸¦ Çü¼ºÇÏ¿´´Ù. NAA´Â ¼¼Ç°Á¾ »Ñ¸®ºÐÈ¿¡ È¿°ú°¡ ¾ø¾úÀ¸¸ç Sunlight ¿Í Chiante ǰÁ¾Àº 1.0 mg/L IAA¿¡¼, Pink Persuasion ǰÁ¾Àº 2.0 mg/L IBA°¡ È¿°úÀûÀ̾ú´Ù. ±«°æÇü¼º¿¡ ¹ÌÄ¡´Â sucrose´Â Sunlight ǰÁ¾Àº 90 g/L, Chiante ¹× Pink Persuasion ǰÁ¾Àº 70 g/L °¡ ±«°æ Çü¼º ¹× ºñ´ë¿¡ È¿°úÀûÀ̾ú´Ù. |
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| To establish rapid micropropagation through organogenesis from apices-derived callus or direct adventitious shoot of three calla lily cultivars(Zantedeschia spp, cv. Sunlight, cv. Chiante, cv. Pink Persuation) were cultured on Murashige and Skoog medium supplemented with different plant growth regulators. The formation rate of callus, organogenesis and in viª«o tuber production among the three cultivars were tested. Callus was obtained from cvs. Sunlight, Chiante and Pink Persuasion; the best cultivar was Sunlight. Sunlight induced $53.3%$ callus and Chiante had the highest rate of $56.7%$ direct shoot regeneration on medium with 2.0 mg/L BA. Regeneration frequencies ranged from 20 to $70%$ on medium with 2.0-3.0 mg/L BA. The highest percentage of regeneration and the greatest number of shoots were obtained on medium containing 3.0 mg/L BA in three cultivars. Cytokinins induced multiple shoot formation; 1.0 mg/L of 2ip, 5.0 mg/L of BA, and 1.0 m/L of BA induced 16, 14 and 12 multiple shoots in cvs. Sunlight, Chiante and Pink Persuasion, respectivly. 1.0 mg/L of IAA enhanced root growth in cvs. Sunlight and Chiante while cv. Pink Persuasion exhibited enhanced root growth at 2.0 mg/L of IBA. NAA, however, induced no change in root growth. The addition of 90 g/L sucrose enhanced in vitro tuber formation and following tuber expansion in cv. Sunlight, while 70 g/L of sucrose was effective in cvs. Chiante and Pink Persuasion. |
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| Ű¿öµå |
| adventitious shoot formation;growth regulators;in vitro culture;sucrose;tuberization; |
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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸÁö / v.18, no.1, 2005³â, pp.154-160
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200502612642500)
¾ð¾î : Çѱ¹¾î |
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| ³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø |
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