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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.18, no.1, 2005³â, pp.169-178
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ÁßÀ̿ ºöÁ¶»ç ´ã¹è(Nicotiana plumbaginifolia) ½Ä¹°Ã¼ÀÇ »ýÀå°ú DNA º¯ÀÌ
( Growth and DNA Alteration of Heavy-ion Beam Irradiated Tobacco(Nicotiana plumbaginifolia) Plant ) |
| ·ùÀçÀÏ;±è¹Î¼ö;;ÀÌÈ¿¿¬;¾ç´öÃá;¹èâÈÞ; ¼øÃµ´ëÇб³ ³ó¾÷»ý¸í°úÇдëÇÐ ½Ä¹°»ý»ê°úÇкÎ;¼øÃµ´ëÇб³ ³ó¾÷»ý¸í°úÇдëÇÐ ½Ä¹°»ý»ê°úÇкÎ;ÀϺ» ÀÌÈÇבּ¸¼Ò(RIKEN) Áß¾Ó¿¬±¸¼Ò;Á¦ÁÖ´ëÇб³ »ý¸íÀÚ¿ø°úÇдëÇÐ »ý¸í°øÇкÎ;°æÈñ´ëÇб³ ÇѹæÀç·á°¡°ø¼¾ÅÍ;¼øÃµ´ëÇб³ ³ó¾÷»ý¸í°úÇдëÇÐ ½Ä¹°»ý»ê°úÇкÎ;
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| ÃÊ ·Ï |
| ÁßÀ̿ºö$(^{20}Ne)$À» ´ã¹è ½Ä¹°Ã¼¿¡ Á¶»ç(irradiation)ÇÏ¿© ½Ä¹°Ã¼ÀÇ »ýÀå°ú DNAÀÇ º¯ÀÌ¿¡ ¹ÌÄ¡´Â ¿µÇâÀ» °ËÅäÇÏ¿´´Ù. ¹ß¾ÆÀ²°ú ÃÊÀåÀº Á¶»ç¼± ·®ÀÌ Áõ°¡ÇÔ¿¡ µû¶ó °¨¼ÒÇÏ¿´´Ù. ¹Ý¸é $50Gy~10Gy$ÀÇ Àú¼±·®¿¡¼ Ãß´ë¿Í °³È°¡ ÃËÁøµÇ¾î ¹ßÀ°Àº ¿ÀÈ÷·Á ÃËÁøµÇ¾ú´Ù. ÃÑ 100°³ÀÇ primer¸¦ ÀÌ¿ëÇÏ¿© RAPDºÐ¼®ÇÑ °á°ú, 59°³ÀÇ primer¿¡¼ ÃÑ 336°³ÀÇ DNA´ÜÆíÀÌ ÁõÆøµÇ¾ú°í, 1°³ÀÇ primer¿¡¼ ÁßÀ̿ºö Á¶»ç󸮱¸¿¡¼¸¸ ÃâÇöÇÏ´Â DNA´ÜÆíÀÌ ³ªÅ¸³µ´Ù. AFLPºÐ¼® °á°ú, °³Ã¼ ƯÀÌÀûÀÎ DNA´ÜÆíÀº ³ªÅ¸³ ¹Ý¸é ÁßÀ̿ºö 󸮱¸ ƯÀÌÀûÀÎ DNA ´ÜÆíÀº °üÂûµÇÁö ¾Ê¾Ò´Ù. |
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| Effects of heavy-ion beam$(^{20}Ne)$ irradiation on growth and DNA alteration of tobacco plants were investigated. Seed germination and plant height were decresed as the ion-beam intensity was increased. However, the bolting and flowering were promoted by the low intensities of 5 Gy to 10 Gy treatment. Out of the 100 primers screened, 59 primers generated 336 DNA fragments by RAPD analysis, and one specific DNA fragment that amplified in control but not in the ion-beam irradiated plants was observed. By AFLP analysis, DNA fragment difference related to the ion-beam treatment was not detected but observed among the plant bodys. |
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| Ű¿öµå |
| AFLP;DNA alteration;heavy-ion beam;Nicotiana plumbaginifolia;RAPD; |
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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸÁö / v.18, no.1, 2005³â, pp.169-178
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200502612642613)
¾ð¾î : Çѱ¹¾î |
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| ³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø |
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