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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.9, no.3, 1996³â, pp.203-210
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»ýÀ°È¯°æÀÌ ´õ´ö(Codonopsis lanceolata)ÀÇ Çâ»êÈÈ¿¼Ò Ȱ¼º¿¡ ¹ÌÄ¡´Â ¿µÇâ
( The effect of Cultivated Environments on the Antioxidant Enzyme Activities of Codonopsis lanceolata ) |
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| ´õ´ö »Ñ¸® ³»¿¡ ÇÔÀ¯µÇ¾î ÀÖ´Â Ç×»êÈÈ¿¼Ò¸¦ »ýÀ°È¯°æ¿¡ µû¶ó Á¶»çÇϱâ À§ÇÏ¿© °æºÏ ¼º®Áö¿ªÀÇ 1 2³â»ý ¹ç´õ´ö, ¾ß»ý´õ´ö ¹× ³í¿¡¼ Àç¹èµÇ´Â 1 2³â»ý ´õ´öÀ» äÃëÇÏ¿© »ç¿ëÇÏ¿´°í, ºÎÀ§º° ºñ±³´Â °¢ ´õ´ö »Ñ¸®¸¦ upper, middle, low·Î ³ª´©¾î¼ »ç¿ëÇÏ¿´´Ù. À̵éÀ» O radical caseadeÀÎ SOD, POD, CAT activity¸¦ Á¶»çÇÏ¿© ´ÙÀ½°ú °°Àº °á°ú¸¦ ¾ò¾ú´Ù. 1. SOD Ȱ¼ºÀº ¹çÀç¹èÁ¶°ÇÀÇ °æ¿ì 1³â»ýº¸´Ù´Â 2³â»ýÀÌ, ³íÀç¹è½Ã´Â 1³â»ýÀÌ 2³â»ýº¸´Ù´Â ³ôÀº Ȱ¼ºÀ» ³ªÅ¸³»¾úÀ¸¸ç, ¾ß»ýÁ¾ÀÇ ºñȰ¼ºÀÌ 96069u/mg proteinÀ¸·Î °¡Àå ³ôÀº Ȱ¼ºÀ» ³ªÅ¸³»¾ú´Ù. ºÎÀ§º°·Î ºñ±³Çغ¸¸é Àç¹èÁ¾Àº upper>middle>lower ¼øÀ¸·Î ³íÀç¹è½Ã´Â low>upper>middle ¼øÀ¸·Î ¾ß»ýÁ¾Àº middle>lower, upper ¼øÀ¸·Î »ýÀ°È¯°æ¿¡ µû¶ó Â÷À̸¦ ³ªÅ¸³»¾ú´Ù. 2. POD ºñȰ¼º(u/mg protein)Àº ³â±Ù¼ö°¡ ³ôÀ»¼ö·Ï Ȱ¼ºÀº Áõ°¡Çϸç, ¾ß»ýÁö¿ªÀÌ 68 u/mg protein·Î °¡Àå ³ôÀº Ȱ¼ºÀ» ³ªÅ¸³»¾ú´Ù. ºÎÀ§º°·Î´Â middle>lower>upper ¼øÀ¸·Î Ȱ¼ºÀÌ ³ªÅ¸³µÀ¸¸ç, ¼º®¾ß»ýÀÇ middle ºÎºÐÀÌ 85 u/mg protein¸¦ ³ªÅ¸³»¾ú´Ù. 3. CAT Ȱ¼ºÀº POD Ȱ¼º°ú´Â ´Þ¸® ³â±Ù¼ö°¡ ³·À»¼ö·Ï ³ô°Ô ³ªÅ¸³µÀ¸¸ç ¹çÀç¹èÁö¿Í ¾ß»ýÁöÀÇ È°¼ºÀº ºñ½ÁÇÏ¸ç ³íÀç¹èÁö´Â »ó´ëÀûÀ¸·Î ±× Ȱ¼ºÀÌ ³·¾Ò´Ù. ºÎÀ§º°·Î´Â upper>low>middle ¼øÀ¸·Î upper ºÎÀ§°¡ »ó´çÈ÷ ³ôÀº Ȱ¼ºÀ» ³ªÅ¸³»¸ç, ¼º®Àç¹è 1³â»ýÀÇ upper°¡ 53.59 u/mg proteinÀ¸·Î °¡Àå ³ô°Ô ³ªÅ¸³µ´Ù. 4. MS1D(MS+2.4-D 1ppm)¿¡ ¹è¾çÇÑ ¹è¾ç¼¼Æ÷ÀÇ Ç×»êÈÈ¿¼ÒÀÇ È°¼ºÀº CAT´Â 1564, POD´Â 30, SOD´Â 22200 u/mg protein·Î »ýü¿¡ ºñÇØ ³·Àº Ȱ¼ºÀ» ³ªÅ¸³»¾ú´Ù.¸ðµÎ ±«±ÙÀå(ÎÔÐÆíþ)°ú Á¤(ïá)ÀÇ »ó°ü(ßÓμ)ÀÌ, ºÐ±â¼ö(ÝÂÐ÷â¦)¿Í´Â ºÎ(ݶ)ÀÇ »ó°ü(ßÓμ)ÀÌÀÖ¾ú´Ù.(Û¡ä´ëÒ)ÀÌ 98.1%ÀÌ´ø °ÍÀÌ 68.8%·Î °¨¼Ò(Êõá´)ÇÏ¿´´Ù. 3. ¿Âµµº¯È(è®ÓøÜ¨ûù)¿¡ µû¸¥ ¼ö¼öÀÇ ¹ß¾Æ¼Ò¿äÀϼö(Û¡ä´á¶é©ìíâ¦)´Â 12ÀÏ(ìí)($15/10^{circ}C$), 6ÀÏ(ìí)($25/20^{circ}C$) ¹× 3ÀÏ(ìí)($40/35^{circ}C$)·Î ¿Âµµ(è®Óø)°¡ »ó½Â(ß¾ã°)ÇÔ¿¡ µû¶ó ºñ·ÊÀû(ÝïÖÇîÜ)À¸·Î ´ÜÃà(Óõê)µÇ¾ú´Ù. ¿Á¼ö¼ö ¼ö´Â 16ÀÏ(ìí), 7ÀÏ(ìí) ¹× 3ÀÏ(ìí)ÀÌ ¼Ò¿ä(á¶é©)µÇ¾ú´Ù.Õá)Àº $24.6{sim}36.7%$·Î¼ °Ç¿±Áß(Ëëç¨ñé)ÀÇ ÇÔ·®(ùßÕá)º¸´Ù ¿ùµîÈ÷ ³ô¾Ò°í Á¶´Ü¹éÁúÇÔ·®(ðØÓ±ÛÜòõùßÕá)Àº $2.0{sim}5.3%$·Î¼ °Ç¿±Áß(Ëëç¨ñé)ÀÇ ÇÔ·®(ùßÕá)º¸´Ù ÇöÀúÈ÷ ³·¾Ò´Ù. Ư(÷å)È÷ P.931ÀÇ °Ç°æÁß(ËëÌìñé)ÀÇ Á¶¼¶À¯ÇÔ·®(ðØàéë«ùßÕá)Àº ´Ù¸¥ ÀÛ¹°(íÂÚª)¿¡ ºñÇØ ÇöÀú(úéîÊ)È÷ ³ôÀº ÆíÀ̾ú´Ù.ÀûÂ÷ÀÌ(åÖîÜó¬×ä)¸¦ ³ªÅ¸³Â´Ù.Êà)¿¡´Â ºÎ(ݶ)(-)ÀÇ »ó°ü(ßÓμ)ÀÌ ÀÖ´Ù.($P{leq}0.01%$). 5. NEL ¹× starch value ȯ°æ¿Âµµ(ü»ÌÑè®Óø)°¡ »ó½Â(ß¾ã°)µÊ¿¡ µû¶ó °¨¼Ò(Êõá´)µÈ´Ù. 4 ¿±±â(ç¨Ñ¢) sorghum½Ä¹°(ãÕÚª)ÀÇ È¯°æ¿Âµµ(ü»ÌÑè®Óø)¸¦ ´Þ¸® ÇÏ¿´À» ¶§ NEL°¡Ä¡(ʤö·)´Â °¢°¢(ÊÀÊÀ) 4.87MJ($30/25^{circ}C$), 5.46MJ($25/20^{circ}C$) ¹× 5.81MJ/kg($18/8^{circ}C$)·Î º¯(ܨ)ÇÏ¿© °í¿Â(ÍÔè®)¿¡¼ net energy lactation ÃàÀû(õëîÝ)ÀÌ Å©°Ô °¨¼Ò(Êõá´)µÇ¾ú´Ù.´Ù. ±×·¯³ª ±â¿Â(Ѩè®)ÀÌ ³·Àº Á¶°Ç(ðÉËì)¿¡¼´Â ¿¹Ãë°ü¸®(çÔö¢Î·×â)¿¡ µû¸¥ ºñ±¸Á¶¼º(ުϰðãàõ) ź¼öȹ°(÷©â©ûù |
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| The activities of the antioxidative enzymes in the roots of Codonopsis lanceolata have been compared depending on the cultivated environments - wildness, cultivate paddy fields and cultivate dry fields - and the parts of the root. In the Codonopsis lanceolata raised in cultivate paddy fields, the activity of SOD was higher in 2 yrs old than 1 yr old, but the activity in 1 yr old was higher than in 2 yrs old for the plants raised in the cultivate dry fields. The specific activity of SOD in wildness plants 86.069unit/mg protein was the highest among plants studied. The tissue distribution of the SOD activity showed differences depending on the enviroment. The highest activity of SOD was shown in the upper part of the root for the cultivate paddy fields, the loewr parts for the cultivate dry fields and middle parts for the wildness. The specific activity of POD was increased with ages of the plants, and that in the wildness was the highest 68 unit/mg protein among the plants studied. The activity of POD in the parts of the roots was shown as middle>lower>upper. The activity of POD in the middle part of the root, rasied in Soebick province was 85 unit/mg protein. The specific activity of CAT was decreased with ages of the plants. The activities of wildness and cultivate paddy fields was similar, but that in cultivate dry fields was lower than others. The tissue distribution in the parts of the roots was upper>lower>middle. The activity of CAT middle part of rasied in the Sebuck area was 5.359 unit/mg protein. The activities antioxidative in the cells cultured in MSID(Murashige and Skoog +2.4-D 1mg/$iota$) was followings: 1564 for CAT. 30 for POD and 22200 unit/mg protein for SOD. These figures were lower than that in in vivo. |
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| Ű¿öµå |
| Superoxide dismutase;Catalase;Peroxidase;Codonopsis lanceolata; |
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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸÁö / v.9, no.3, 1996³â, pp.203-210
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199611920278389)
¾ð¾î : Çѱ¹¾î |
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| ³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø |
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