¶óÆæÆ®¦¢Ä«Æä¦¢ºí·Î±×¦¢´õº¸±â
¾ÆÄ«µ¥¹Ì Ȩ ¸í»çƯ°­ ´ëÇבּ¸½Ç޹æ Á¶°æ½Ç¹« µ¿¿µ»ó°­ÀÇ Çѱ¹ÀÇ ÀüÅëÁ¤¿ø ÇÐȸº° ³í¹®
ÇÐȸº° ³í¹®

Çѱ¹°Ç¼³°ü¸®ÇÐȸ
Çѱ¹°ÇÃà½Ã°øÇÐȸ
Çѱ¹µµ·ÎÇÐȸ
Çѱ¹»ý¹°È¯°æÁ¶ÀýÇÐȸ
Çѱ¹»ýÅÂÇÐȸ
Çѱ¹¼öÀÚ¿øÇÐȸ
Çѱ¹½Ä¹°ÇÐȸ
Çѱ¹½Ç³»µðÀÚÀÎÇÐȸ
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
Çѱ¹ÀܵðÇÐȸ
Çѱ¹Á¶°æÇÐȸ
Çѱ¹Áö¹Ý°øÇÐȸ
Çѱ¹ÇÏõȣ¼öÇÐȸ
Çѱ¹È¯°æ»ý¹°ÇÐȸ
Çѱ¹È¯°æ»ýÅÂÇÐȸ

Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.9, no.3, 1996³â, pp.305-310
DNA marker Áö¹®¹ý¿¡ ÀÇÇÑ Ã볪¹° 5Á¾ (û¿ÁÃë , °³¹ÌÃë , ÂüÃë , ¼ö¸®Ãë , °õÃë)ÀÇ ºñ±³¿¬±¸
( Comparative studies of the five edible mountain vegetables by DNA marker fingerprinting )
À¯±â¾ï; ;
 
ÃÊ ·Ï
À¯¿ë Ã볪¹° 5Á¾·ùÀÇ Á¾°£ À¯»çµµ ¹× Á¾³» º¯À̸¦ ÆÄ¾ÇÇϱâ À§ÇÏ¿© 38°³Ã¼¸¦ ´ë»óÀ¸·Î PCR ¹æ¹ýÀ» ÀÌ¿ëÇÏ¿© ºñ±³ºÐ¼®ÇÏ¿´´Ù. »ç¿ëµÈ 62°¡Áö primer Áß¿¡¼­ 38°³Ã¼ Àüü¿¡¼­ band¸¦ º¸¿©ÁØ °ÍÀº 10°¡Áö¿´°í, À̸¦ ÅëÇØ ¾ò¾îÁø ÃÑ bandÀÇ ¼ö´Â 176°³·Î ³ªÅ¸³µ´Ù. Á¾³»º¯À̴ û¿ÁÃë°¡ 31.1%¿¡ ÇØ´çÇÏ´Â 15°³ÀÇ polymorphic band¸¦ °¡Á® º¯À̰¡ °¡Àå ³·¾ÒÀ¸¸ç °õÃë´Â 61.1%·Î °¡Àå ³ô°Ô ³ªÅ¸³µ´Ù. ±ºÁýºÐ¼® °á°ú Á¶»çµÈ 38°³Ã¼´Â À¯»çµµ 0.93¿¡¼­ 5Á¾ÀÇ ±¸ºÐÀÌ °¡´ÉÇÏ¿´À¸¸ç 38°³Ã¼ °¢°¢Àº À¯»çµµ 0.62-0.99ÀÇ À¯»ç¼ºÀ» °®´Â °ÍÀ¸·Î ³ªÅ¸³µ°í Á¾³»º¯ÀÌ´Â 0.93ÀÌ»óÀ¸·Î ³ô°Ô ³ªÅ¸³ª º¯À̰¡ ÀûÀº °ÍÀ¸·Î ³ªÅ¸³µ´Ù. ÀÌ»óÀÇ °á°ú¿¡¼­ PCR¹æ¹ýÀ» ÀÌ¿ëÇÑ °á°ú°¡ Ã볪¹° 5Á¾·ù¸¦ ±¸ºÐÇϴµ¥ À¯¿ëÇÏ°Ô »ç¿ëµÇ¾úÀ¸¸ç ±ºÁýºÐ¼® °á°ú Á¾³» º¯À̰¡ ¸Å¿ì ³·Àº °ÍÀ¸·Î ³ªÅ¸³µ´Ù.
Five edible mountain vegetables(Saussurea sp. Aster tataricus, A. scaber. Synurus deltoides, Ligularia fischeri) were investigated on the basis of amplified DNA polymorphisms resulted from PCR (polymerase chain reaction) analysis. The sampled plants consisted of 38 individuals in 5 taxa. Only 10 primers out of 62 primers (60 random [10-mer] primers, two 15-mer [M13 core sequence, and (GGAT) sequence]) tested gave rise to polymorphisms in all of the tested plants, producing 176 DAN fragments amplified. Intraspecific polymorphisms found in each taxa showed intraspecies constancy (31.1-61.1%) in the banding patterns of individual plants: Saussurea sp. 31.1%, 15 bands, Aster tataricus, 40.9%, 18 bands, A. scaber. 38.5%, 15 bands. Synurus deltoides, 34.7%, 17 bands, and Ligularia fischeri, 61.1%, 22 brands, respectively. All five species were well classified from each other at the 0.93 level of similarity index value. Intraspecific and interspecific variations were appeared at the levels ranging from 0.62 to 0.99. Based on these results, our PCR analyses support the previous data derived from external morphology of the 5 edible mountain vegetables, but very low levels o intraspecific variations were detected in all of these taxa.
 
Ű¿öµå
PCR;M13 core sequence;$(GGAT)_4$ sequence;DNA polymorphism;
 
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸÁö / v.9, no.3, 1996³â, pp.305-310
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199611920278514)
¾ð¾î : Çѱ¹¾î
³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø
¸ñ·Ïº¸±â
ȸ»ç¼Ò°³ ±¤°í¾È³» ÀÌ¿ë¾à°ü °³ÀÎÁ¤º¸Ãë±Þ¹æÄ§ Ã¥ÀÓÀÇ ÇѰè¿Í ¹ýÀû°íÁö À̸ÞÀÏÁÖ¼Ò ¹«´Ü¼öÁý °ÅºÎ °í°´¼¾ÅÍ
   

ÇÏÀ§¹è³ÊÀ̵¿