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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.9, no.3, 1996³â, pp.305-310
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DNA marker Áö¹®¹ý¿¡ ÀÇÇÑ Ã볪¹° 5Á¾ (û¿ÁÃë , °³¹ÌÃë , ÂüÃë , ¼ö¸®Ãë , °õÃë)ÀÇ ºñ±³¿¬±¸
( Comparative studies of the five edible mountain vegetables by DNA marker fingerprinting ) |
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| À¯¿ë Ã볪¹° 5Á¾·ùÀÇ Á¾°£ À¯»çµµ ¹× Á¾³» º¯À̸¦ ÆÄ¾ÇÇϱâ À§ÇÏ¿© 38°³Ã¼¸¦ ´ë»óÀ¸·Î PCR ¹æ¹ýÀ» ÀÌ¿ëÇÏ¿© ºñ±³ºÐ¼®ÇÏ¿´´Ù. »ç¿ëµÈ 62°¡Áö primer Áß¿¡¼ 38°³Ã¼ Àüü¿¡¼ band¸¦ º¸¿©ÁØ °ÍÀº 10°¡Áö¿´°í, À̸¦ ÅëÇØ ¾ò¾îÁø ÃÑ bandÀÇ ¼ö´Â 176°³·Î ³ªÅ¸³µ´Ù. Á¾³»º¯À̴ û¿ÁÃë°¡ 31.1%¿¡ ÇØ´çÇÏ´Â 15°³ÀÇ polymorphic band¸¦ °¡Á® º¯À̰¡ °¡Àå ³·¾ÒÀ¸¸ç °õÃë´Â 61.1%·Î °¡Àå ³ô°Ô ³ªÅ¸³µ´Ù. ±ºÁýºÐ¼® °á°ú Á¶»çµÈ 38°³Ã¼´Â À¯»çµµ 0.93¿¡¼ 5Á¾ÀÇ ±¸ºÐÀÌ °¡´ÉÇÏ¿´À¸¸ç 38°³Ã¼ °¢°¢Àº À¯»çµµ 0.62-0.99ÀÇ À¯»ç¼ºÀ» °®´Â °ÍÀ¸·Î ³ªÅ¸³µ°í Á¾³»º¯ÀÌ´Â 0.93ÀÌ»óÀ¸·Î ³ô°Ô ³ªÅ¸³ª º¯À̰¡ ÀûÀº °ÍÀ¸·Î ³ªÅ¸³µ´Ù. ÀÌ»óÀÇ °á°ú¿¡¼ PCR¹æ¹ýÀ» ÀÌ¿ëÇÑ °á°ú°¡ Ã볪¹° 5Á¾·ù¸¦ ±¸ºÐÇϴµ¥ À¯¿ëÇÏ°Ô »ç¿ëµÇ¾úÀ¸¸ç ±ºÁýºÐ¼® °á°ú Á¾³» º¯À̰¡ ¸Å¿ì ³·Àº °ÍÀ¸·Î ³ªÅ¸³µ´Ù. |
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| Five edible mountain vegetables(Saussurea sp. Aster tataricus, A. scaber. Synurus deltoides, Ligularia fischeri) were investigated on the basis of amplified DNA polymorphisms resulted from PCR (polymerase chain reaction) analysis. The sampled plants consisted of 38 individuals in 5 taxa. Only 10 primers out of 62 primers (60 random [10-mer] primers, two 15-mer [M13 core sequence, and (GGAT) sequence]) tested gave rise to polymorphisms in all of the tested plants, producing 176 DAN fragments amplified. Intraspecific polymorphisms found in each taxa showed intraspecies constancy (31.1-61.1%) in the banding patterns of individual plants: Saussurea sp. 31.1%, 15 bands, Aster tataricus, 40.9%, 18 bands, A. scaber. 38.5%, 15 bands. Synurus deltoides, 34.7%, 17 bands, and Ligularia fischeri, 61.1%, 22 brands, respectively. All five species were well classified from each other at the 0.93 level of similarity index value. Intraspecific and interspecific variations were appeared at the levels ranging from 0.62 to 0.99. Based on these results, our PCR analyses support the previous data derived from external morphology of the 5 edible mountain vegetables, but very low levels o intraspecific variations were detected in all of these taxa. |
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| Ű¿öµå |
| PCR;M13 core sequence;$(GGAT)_4$ sequence;DNA polymorphism; |
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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸÁö / v.9, no.3, 1996³â, pp.305-310
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199611920278514)
¾ð¾î : Çѱ¹¾î |
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| ³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø |
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