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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.13, no.1, 2000³â, pp.48-53
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( Development of Aspceptic Seedling by In Vitro Germination in Lacquer Tree Seed ) |
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| ¿Ì³ª¹« ¹¦¸ñ´ë·®Áõ½Ä¹ýÀÇ Çϳª·Î Á¶Á÷¹è¾ç ¹æ¹ýÀ» ÀÌ¿ëÇÏ¿© ±â³» À¯½Ä¹°Ã¼¸¦ ´Ù·®À¸·Î Áõ½ÄÇÔÀ¸·Î½á ¿Ì³ª¹« Áõ½ÄÀÇ È¿À²¼ºÀ» ³ôÀ̱â À§ÇÏ¿© ±â³» ¹«±Õ¹¦¸¦ ¾ò°íÀÚ ½Ç½ÃÇÏ¿´´Ù. ºñÁß 1.0À¸·Î ¼±Á¾ÇÑ °á°ú 1Â÷¼±Á¾Àº Æò±Õ 50.7%ÀÇ ¼±Á¾À²À» º¸¿´°í, 98% sulfuric acid¿¡ 2½Ã°£ ±³¹Ý ÈÄ 2Â÷·Î ¼±Á¾ÇÑ °á°ú ¼±Á¾À²Àº 20.8%¿´´Ù. ¹ß±ÙÀ²Àº ¿ÜÁ¾ÇǸ¦ ÇÔÀ¯ÇÑ Á¾ÀÚ(5.4%) º¸´Ù´Â ¿ÜÁ¾ÇǸ¦ Á¦°ÅÇϰųª(18.3%) ³»Á¾ÇǸ¦ Á¦°ÅÇÑ Á¾ÀÚ(32.4%)°¡ ³ô¾Ò´Ù. Àü󸮿¡ µû¸¥ Á¾ÀÚÀÇ ¹ß¾ÆÀ²Àº »ýÀåÁ¶Àý¹°Áú 󸮿¡ ÀÇÇÑ È¿°ú°¡ ÀÎÁ¤µÇÁö ¾Ê¾ÒÀ¸¸ç, °¢ 󸮺° À¯ÀǼöÁصµ ÀÎÁ¤µÇÁö ¾Ê¾ÒÀ¸³ª sulfuric acid¸¦ ó¸®ÇßÀ» °æ¿ì ¹ß¾ÆÀ²ÀÌ 3%·Î½á »ýÀåÁ¶Àý¹°Áú 󸮺¸´Ù´Â ¾à°£ È¿°úÀûÀÎ °ÍÀ¸·Î ³ªÅ¸³µ´Ù. ¿ÜÁ¾ÇǸ¦ Á¦°ÅÇÏ¸é ¹ß¾ÆÀ²Àº ¾à°£ ³ô°Ô ³ªÅ¸³µ´Âµ¥, »ýÀåÁ¶Àý¹°ÁúÀ» ÷°¡ÇÔÀ¸·Î½á ¾à 4%Á¤µµ Çâ»óµÇ¾ú´Ù. ƯÈ÷ BA 1.0mg/L¿Í NAA 0.05mg/LÀÇ È¥¿ëó¸®¿Í BA 1.0mg/L ´Üµ¶Ã³¸®°¡ 10% ³»¿ÜÀÇ ¹ß¾ÆÀ²À» º¸¿´´Ù. ¿Ì³ª¹« Á¾ÀÚ´Â ¹èÁö¿¡ Ä¡»óÇÑ ÈÄ ¾à 10ÀÏ ¸¸¿¡ Á¤»óÀûÀ¸·Î ¹ß¾ÆÇÏ¿´°í, Ä¡»ó ÈÄ ¾à 3ÁÖ¸¸¿¡ º»¿±ÀÌ ÃâÇöÇÑ Á¤»óÀûÀÎ ¹«±Õ¹¦¸¦ ȹµæÇÒ ¼ö ÀÖ¾úÀ¸³ª ¹ß¾ÆÀ²ÀÌ 10%³»¿Ü¿¡ ºÒ°úÇÏ¿© ¸Å¿ì ÀúÁ¶ÇÏ¿´´Ù. |
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| Lacquer tree can be proliferated by root or stem cutting, and seed. In case of proliferation by seed, however, the germination rate is very low. Thus, the present study was carried out to obtain aspceptic lacquer plant in vitro from seed because natural tissue culture was highly defiled by unknown fungi and bacteria. First seed grading on distilled water was 50.7% and second seed grading was 20.8% after 98% sulfuric acid treatment for 2 hours. Removal of inner seed coat was higher with 32.4% than non-removal of outer seed coat and removal outer seed coat in rooting rate. In germination rate according to pre-treatment, growth regulators were not effective at all, but sulfuric acid was effective a little with 3%. Removal outer seed coat was increased about 4%, that germinated about 10% in MS medium supplemented with 1.0mg/L BA and 0.05mg/L NAA, 1.0mg/L BA. Lacquer tree seeds germinated after 10 days in MS medium, and aspceptic seedling of lacquer tree were obtained after 3 weeks in vitro. Germination rate, however, was lower about 10%. |
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| Ű¿öµå |
| acquer tree;seed grading;germination;growth regulators;sulfuric acid;aspceptic seedling in vitro; |
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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸÁö / v.13, no.1, 2000³â, pp.48-53
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200011921402560)
¾ð¾î : Çѱ¹¾î |
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| ³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø |
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