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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.23, no.5, 2010³â, pp.436-444
¾çÄ¡½Ä¹°·ùÀÇ ¸Þź¿Ã ÃßÃâ¹°¿¡ Ç×±ÕȰ¼º ºÐ¼®
( Antimicrobial Activities of Methanol Extracts Obtained from Several Ferns )
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10Á¾ ¾çÄ¡½Ä¹°ÀÇ Áö»óºÎ¿Í ÁöÇϺΠ¸Þź¿Ã ÃßÃâ¹°À» ´ë»óÀ¸·Î Bacillus subtilis, Escherichia coli, Listeria monocytogenes, Propionibacterium acnes, Streptococcus mutans, Streptococcus sobrinus µî º´¿ø¼º ¹Ì»ý¹°¿¡ ´ëÇÑ Ç×±ÕȰ¼ºÀ» broth microdilution¹ý°ú paper diffusion¹ýÀ» ÀÌ¿ëÇÏ¿© ºÐ¼®ÇÏ¿´´Ù. ¾ïÁ¦È¯ÀÌ 15 mm ÀÌ»óÀ¸·Î ³ªÅ¸³­ ÃßÃâ¹°À» ´ë»óÀ¸·Î 0.125, 0.25, 0.5 1, $2;mg{cdot}mL^{-1}$ÀÇ ³óµµ¿¡¼­ 3ÀÏ µ¿¾È °¢ ¹Ì»ý¹°ÀÇ »ýÀ° ¾ïÁ¦¿¡ ¹ÌÄ¡´Â ¿µÇâÀ» ºÐ¼®ÇÏ¿´´Ù. ±× °á°ú, B. subtilis, E. coli, L. monocytogenes¿¡ ´ëÇØ¼­´Â ²æ°íºñÀÇ ÁöÇϺΠÃßÃâ¹°¿¡¼­ ¾ïÁ¦È°¼ºÀÌ °¡Àå ¿ì¼öÇÏ¿´À¸¸ç, »ì±Õó¸®ÇÏÁö ¾ÊÀº Á¶ÃßÃâ¹° »óÅ¿¡¼­ $2;mg{cdot}mL^{-1}$ÀÇ ³óµµ¿¡¼­ 72½Ã°£ ÀÌÈÄ¿¡µµ °¢ 39, 33, 58%ÀÇ ¾ïÁ¦È°¼ºÀ» º¸¿´´Ù. P. acnes¿¡ ´ëÇØ¼­´Â ²æ°íºñ Áö»óºÎ ÃßÃâ¹°ÀÌ °¡Àå ¿ì¼öÇÑ Ç×±ÕȰ¼ºÀ» º¸¿´À¸³ª, 15 mm ¹Ì¸¸ÀÇ ¾ïÁ¦È¯À» º¸¿© Ç×±Õ·ÂÀÌ ¾àÇÑ °ÍÀ¸·Î ³ªÅ¸³µ´Ù. S. mutans¿Í S. sobrinus¿¡ ´ëÇØ¼­´Â ´Ù¼öÀÇ ¾çÄ¡½Ä¹°ÀÌ ºñ±³Àû ¿ì¼öÇÑ Ç×±ÕȰ¼ºÀ» º¸¿© ¾çÄ¡½Ä¹°ÀÇ ÃßÃâ¹°Àº Àü¹ÝÀûÀ¸·Î Streptococcus¿¡ ´ëÇÑ °¨¼ö¼ºÀÌ ³ôÀº °ÍÀ¸·Î »ý°¢µÇ¾ú´Ù. ±×·¯³ª °íºñÀÇ ÁöÇϺΠÃßÃâ¹°ÀÌ $2;mg{cdot}mL^{-1}$ÀÇ ³óµµ¿¡¼­ 72½Ã°£ ÈÄ °¢ 28, 39%, ±× ¿ÜÀÇ ÃßÃâ¹°Àº 10% ³»¿ÜÀÇ ³·Àº Ç×±ÕȰ¼ºÀ» º¸¿© 10Á¾ÀÇ ¾çÄ¡½Ä¹° ÃßÃâ¹° Áß¿¡´Â Streptococcus¿¡ ´ëÇÏ¿© ¶Ù¾î³­ Ç×±Õ·ÂÀ» º¸ÀÌ´Â ÃßÃâ¹°Àº ¾ø´Â °ÍÀ¸·Î »ý°¢µÇ¾ú´Ù.
Methanol extracts of the aerial and rhizome parts obtained from ten species of ferns has been screened for antimicrobial activities against Bacillus subtilis, Escherichia coli, Listeria monocytogenes, Propionibacterium acnes, Streptococcus mutans and Streptococcus sobrinus. Antimicrobial activities were carried out using broth microdilution method and paper disc diffusion assay and the extracts which showed clear zones more than 15mm in concentration of 2 mg/disc were tested for its antimicrobial activities at the $0.125{sim}2;mg{cdot}mL^{-1}$ concentration of each extract for three days. The non-sterilized crude methanol extracts of Osmunda cinnamomea var. fokiensis rhizome showed the highest antimicrobial activities on B. subtilis(39%), E. coli (33%) and L. monocytogenes(58%) at the concentration of $2;mg{cdot}mL^{-1}$ after 72 hours. In P. acnes, frond extract of O. cinnamomea var. fokiensis showed most vigorous antimicrobial activities in the all extracts but it showed weak activity(clear zone diameters below 15 mm). All extracts has the antimicrobial activities on Streptococcus, but they exhibited weak activity. At the concentration of $2;mg{cdot}mL^{-1}$, only Osmunda japonica rhizome extracts showed 28 and 39% of antimicrobial activities on S. mutans and S. sobrinus after 72 hours and the other extracts showed below 10% of antimicrobial activities on S. mutans and S. sobrinus.
 
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Bacillus subtilis;Escherichia coli;Listeria monocytogenes;Propionibacterium acnes;Streptococcus;
 
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸÁö / v.23, no.5, 2010³â, pp.436-444
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO201006159731685)
¾ð¾î : Çѱ¹¾î
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