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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.23, no.5, 2010³â, pp.458-464
Çѱ¹°ú ¸ù°í ÀϺΠÀç¹è¸¶´ÃÀÇ À¯ÀüÀû º¯ÀÌ¿Í Àç¹èÁ¾ ƯÀÌÀû RAPD ¸¶Ä¿ÀÇ Å½»ö
( Genetic Variation and Identification of RAPD Markers from Some Garlic Cultivars in Korea and Mongolia )
¹è¼º±¹;Á¤Àº¾Æ;±Ç¼øÅÂ; ¾Èµ¿´ëÇб³ ¿ø¿¹À°Á¾Çаú;¾Èµ¿´ëÇб³ ¿ø¿¹À°Á¾Çаú;¾Èµ¿´ëÇб³ ¿ø¿¹À°Á¾Çаú;
 
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±¹³»¿Ü¿¡¼­ Àç¹èµÇ´Â 12Á¾ÀÇ ¸¶´ÃÀ» ¼öÁýÇÏ¿© ÃÑ 143°³ÀÇ ÀÓÀÇÀÇ primer¸¦ ÀÌ¿ëÇÏ¿© RAPDºÐ¼®À» ½Ç½ÃÇÑ °á°ú 55°³ÀÇ primer·ÎºÎÅÍ Á¾°£¿¡ ´ÙÇü¼ºÀ» º¸ÀÌ´Â DNA¹êµå°¡ ³ªÅ¸³µ´Ù. RAPD¿¡ ÀÇÇØ ´ÙÇü¼ºÀ» º¸ÀÎ 55°³ÀÇ primer¿¡¼­ È®ÀÎµÈ ÃÑ DNA ¹êµå ¼ö´Â 187°³¿´À¸¸ç, ±× Áß 128°³(68.5%)°¡ 12Á¾ÀÇ ¸¶´Ã Áö¹æÁ¾°£¿¡ ´ÙÇü¼ºÀ» ³ªÅ¸³»¾ú´Ù. PCR¿¡¼­ ´ÙÇü¼ºÀ» º¸ÀÎ DNA ¹êµå¸¦ ´ë»óÀ¸·Î Áý´ÜºÐ¼®À» ½Ç½ÃÇÑ °á°ú À¯ÀüÀû À¯»çµµ°¡ 0.71À̻󿡼­ 3°³ÀÇ ±×·ìÀ¸·Î ³ª´©¾î Á³´Âµ¥, Á¦1±×·ìÀº ÀǼº, ¼­»ê, »ïô, ¿¹Ãµ-A, ¿¹Ãµ-BÁ¾, ÀǼº³ë¶û, Á¤¼±, ³²µµ, ´Ü¾ç ¹× À°¹éÁ¾ µîÀ¸·Î ´ë¼­Á¾À» Á¦¿ÜÇÑ Çѱ¹ÀÇ Àç¹èÁ¾ÀÌ ¸ðµÎ Æ÷ÇԵǾúÀ¸¸ç, Á¦2±×·ì°ú Á¦3±×·ìÀº °¢°¢ ¸ù°ñÁ¾°ú ´ë¼­Á¾ ´Üµ¶À¸·Î ³ª´©¾îÁ³´Ù. Á¾ ƯÀÌÀûÀ¸·Î DNA¹êµå¸¦ ³ªÅ¸³»´Â primer¸¦ ºÐ¼®ÇÑ °á°ú 21°³ primer¿¡¼­ 30°³ÀÇ DNA¹êµå°¡ ¾î´À ƯÁ¤ÀÇ Áö¹æÁ¾¿¡¸¸ ³ªÅ¸³ª´Â °ÍÀ¸·Î È®ÀεǾî, Áö¹æÁ¾ ¸¶´Ã 10Á¾À» ±¸ºÐÇÒ ¼ö ÀÖ´Â 30°³ÀÇ RAPD ¸¶Ä¿°¡ È®ÀεǾú´Ù.
Twelve garlic cultivars collected from Korea and Mongolia were evaluated genetic similarity and diversity by RAPD method using oligo-nucleotide random primers. Genomic DNA isolated from twelve garlic cultivars were amplified by polymerase chain reaction using 143 primers, and 55 primers showed polymorphic DNA bands. Among a total of 187 bands amplified by 55 primers, 128 polymorphic bands were subjected to analysis for genetic relationship of garlic cultivars. Garlic cultivars were classified into three groups, such as group-I corresponded to Euiseong, Seosan, Samchuk and Yecheon-A, Yechun-B, Euiseong-norang, Jeongsun, Namdo, Yookback and Danyang cultivars, and group-II to Mongolia and group-III to Daeseo cultivars. Thirty DNA bands showing unique specificity to the specific cultivars are likely to be useful for identification of garlic local cultivars as DNA markers.
 
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PCR;RAPD;polymorphic bands;identification of cultivars;
 
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸÁö / v.23, no.5, 2010³â, pp.458-464
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO201006159731691)
¾ð¾î : Çѱ¹¾î
³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø
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