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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.24, no.4, 2011³â, pp.456-460
¸¸º´ÃÊ(Rhododendron brachycarpum) ÃßÃâ¹°ÀÇ Ç×»êÈ­ È¿°ú
( The Antioxidative Effects of Rhododendron brachycarpum Extracts )
ÀÓÅÂÁø;ÃÖ¹«¿µ; »óÁö´ëÇб³ µ¿¹°»ý¸íÀÚ¿øÇкΠ»ý¸í°øÇÐ;»óÁö´ëÇб³ ½Äǰ¿µ¾çÇаú;
 
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º» ¿¬±¸¿¡¼­´Â ¸¸º´ÃÊ 95% ¿¡Åº¿ÃÃßÃâ¹°ÀÇ Ç×»êÈ­ È¿°ú¸¦ Á¶»çÇÏ¿´´Ù. ÃÑÇ×»êÈ­ Ȱ¼ºÀº ABTS ¶óµðÄ®¿¡ ´ëÇÑ Ç×»êÈ­´ÉÀ¸·Î ÃøÁ¤ÇÏ¿´´Ù. ¸¸º´ÃÊ ÃßÃâ¹° 0.2 ¹× 1 mg/mLÀÇ ÃÑ Ç×»êÈ­´ÉÀº °¢°¢ 0.33 ¹× 2.26 mM Trolox¿Í µ¿µîÇÑ ¼öÁØÀ̾ú´Ù. ¸¸º´ÃÊ ÃßÃâ¹° 0.2 ¹× 1 mg/mLÀÇ superoxide ¼Ò°Å´ÉÀº °¢°¢ 45.0 ¹× 77.0%À̾ú´Ù. ¸¸º´ÃÊ ÃßÃâ¹° 5 ¹× 100 ${mu}g/mL$ÀÇ »ê¼Ò¶óµðÄ® ¼Ò°Å´ÉÀº °¢°¢ 40.88 ¹× 131.00 ${mu}M$ Trolox¿Í µ¿µîÇÑ ¼öÁØÀ̾ú´Ù. ¸¸º´ÃÊ ÃßÃâ¹° 0.2 ¹× 1 mg/mLÀÇ ÃÑÆä³î ÇÔ·®Àº °¢°¢ 0.37 ¹× 1.25 mM gallic acid¿Í µ¿µîÇÑ ¼öÁØÀ̾ú´Ù. ¶ÇÇÑ, HepG2 ¼¼Æ÷ÁÖ¸¦ ÀÌ¿ëÇÑ ¼¼Æ÷¹è¾ç¿¡¼­ ¸¸º´ÃÊ ÃßÃâ¹° 0.1 mg/mL ³óµµÀÇ Ã·°¡´Â 0.2 ¹× 0.5 mM tert-butyl hydroperoxide·Î À¯µµµÈ ¼¼Æ÷µ¶¼ºÀ» °¢°¢ 52.1 ¹× 30.3% °¨¼Ò½ÃÄ×´Ù. µû¶ó¼­, º» ¿¬±¸ °á°úµéÀº ¸¸º´ÃÊ ÃßÃâ¹°ÀÇ °­·ÂÇÑ Ç×»êÈ­ È¿°ú¿Í ¼¼Æ÷µ¶¼º ¾ïÁ¦ È¿°ú¸¦ ³ªÅ¸³»¸ç, ÀÌ·¯ÇÑ È¿´ÉÀº Àû¾îµµ ÀÚÀ¯¶óµðÄ®ÀÇ »êÈ­ ¾ïÁ¦¿Í ³ôÀº ÃÑÆä³î ÇÔ·®¿¡ ±âÀÎÇÏ´Â °ÍÀ¸·Î »ç·áµÈ´Ù.
The objective of this study was to investigate the antioxidative capacity of Rhododendron brachycarpum 95% ethanol extracts. Total antioxidant status was examined by total antioxidant capacity against ABTS radical reactions. Total antioxidant capacities of R. brachycarpum extract at the concentrations of 0.2 and 1 mg/mL were 0.33 and 2.26 mM Trolox equivalents, respectively. Superoxide scavenging activities of R. brachycarpum extract at the concentrations of 0.2 and 1 mg/mL were 45.0 and 77.0%, respectively. Oxygen radical absorbance capacities of R. brachycarpum extract at the concentrations of 5 and 100 ${mu}g/mL$ were 40.88 and 131.00 ${mu}M$ Trolox equivalents, respectively. Total phenolic contents of R. brachycarpum extract at the concentrations of 0.2 and 1 mg/mL were 0.37 and 1.25 mM gallic acid equivalents, respectively. R. brachycarpum extract at the concentration of 0.1 mg/mL inhibited 0.2 mM and 0.5 mM tert-butyl hydroperoxide induced cyototoxicity by 52.1 and 30.3%, respectively, in HepG2 cell culture system. Thus, strong antioxidant and cytotoxicity-inhibiting effects of R. brachycarpum extract seem to be due to, at least in part, the prevention from free radicals-induced oxidation as well as high levels in total phenolic contents.
 
Ű¿öµå
Rhododendron brachycarpum;ABTS radical;Superoxide;ORAC;Cytotoxicity;
 
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸÁö / v.24, no.4, 2011³â, pp.456-460
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO201125136122670)
¾ð¾î : Çѱ¹¾î
³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø
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