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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ / v.24, no.5, 2011³â, pp.499-506
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°ËÁ¤µ¿ºÎ Á¾ÇÇ¿¡¼ÀÇ »ö¼Ò ÃßÃâ°ú ÃßÃâ»ö¼ÒÀÇ ÀúÀå ¾ÈÁ¤¼º ¿¬±¸
( Research on the Colorants Extraction from Black Cowpea Seed Coats and their Storage Stability ) |
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| º» ¿¬±¸¿¡¼´Â °ËÁ¤ µ¿ºÎ Á¾ÇÇ¿¡ ÇÔÀ¯µÈ õ¿¬»ö¼Ò¿¡ ´ëÇÑ ¿¬±¸ÀÇ ÀÏȯÀ¸·Î, ÃßÃâ¹æ¹ý¿¡ µû¸¥ »ö¼Ò ÃßÃâ È¿À²°ú ÀúÀå¾ÈÁ¤Á¦ ÷°¡¿¡ µû¸¥ ÀúÀå ¾ÈÁ¤¼º°ú Ư¼ºÀ» °ËÅäÇÏ¿© ´ÙÀ½°ú °°Àº °á°ú¸¦ ¾ò¾ú´Ù. 1) ¿ë¾× ÃßÃ⠽à ¿Âµµ°¡ ³ôÀ»¼ö·Ï, ½Ã°£ÀÌ °æ°úµÉ¼ö·Ï »ö¼Ò ÃßÃâ È¿À²ÀÌ ³ô°Ô ³ªÅ¸³µÀ¸¸ç, pH Á¶°Ç¿¡¼´Â pH 4°¡ °¡Àå È¿À²ÀûÀ̾ú´Ù. 2) Âø¾× ÃßÃâ ½Ã, ´Ü½Ã°£ Àü󸮿¡¼´Â pH 4°¡ pH 5¿¡¼º¸´Ù »ö¼Ò ÃßÃâ·®ÀÌ ¸¹¾ÒÁö¸¸, 15½Ã°£ ÀÌ»óÀÇ Àå½Ã°£ Àü󸮿¡¼´Â ¿ÀÈ÷·Á pH 5¿¡¼ ¸¹°Ô ³ªÅ¸³µ´Ù. 3) °ËÁ¤µ¿ºÎ »ö¼Ò¿ë¾×ÀÇ pH º¯È¿¡ µû¸¥ »öµµ´Â, orange-red»ö(pH 3.0), orange»ö(pH 4.0~6.0), blue»ö(pH 7.0) ±×¸®°í greenish-blue»ö(pH 9.0~11.0)À¸·Î Antocyanin°ú À¯»çÇÑ °Åµ¿À» º¸¿´´Ù. 4) ÀúÀå ¾ÈÁ¤Á¦·Î MethionineÀ» ÷°¡ÇÑ °á°ú¿¡¼´Â ÷°¡¿¡ µû¸¥ °ËÁ¤ µ¿ºÎ »ö¼ÒÀÇ ¾ÈÁ¤È È¿°ú¿¡¼ ÀúÀå ±â°£ 18ÀϱîÁö´Â º¯»ö Áö¿¬ È¿°ú¸¦ º¸¿´À¸¸ç, Methionine 10 mmol ÷°¡>20 mmol ÷°¡>¹«Ã·°¡ ¼øÀ¸·Î ¾ÈÁ¤È¿°ú°¡ Å©°Ô ³ªÅ¸³µ´Ù. 5) °ËÁ¤ µ¿ºÎ Á¾ÇǷκÎÅÍ Âø¾× ÃßÃâÇÑ »ö¼Ò ºÐ¸»À» paper chromatographyÇÑ °á°ú, ÇϳªÀÇ ±ä ¶ì ¸ð¾çÀ¸·Î Àü°³µÇ¾úÀ¸¸ç Rf °ªÀº °¢°¢ 0.68(BAW), 0.53(30% acetic acid)À¸·Î ³ªÅ¸³µ´Ù. 6) Chromatograph paper»ó¿¡¼ Àü°³¼Óµµ¿¡ µû¶ó 3ºÐȹÀ¸·Î ±¸ºÐÇÏ¿© ÃëÇÑ »ö¼ÒÀÇ spectrum ºÐ¼®¿¡¼´Â 552 nmÀÇ shoulderÀÇ Á¸Àç À¯¹«¸¦ ±Ù°Å·Î Àû¾îµµ 2°¡Áö ÀÌ»óÀÇ »ö¼Ò ¼ººÐÀÌ È¥ÇյǾî ÀÖ´Â °ÍÀ» ¸íÈ®È÷ ¾Ë ¼ö ÀÖ¾ú´Ù. |
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| The purpose of this study was to search available resources for new natural colorants. The extraction efficiency of colorants from black cowpea seed coats and their storage stability were examined according to the various extraction and storage conditions in this study. The results obtained were as follows: the optical density (O.D.) values of the extracted colorants increased with increasing extract time and temperature. Extraction at pH 4 was seen to be the most efficient among the various pH conditions. The color of the extract solutions were seen to change with variation in pH, for example, anthocyanins display color changes from orange-red, to orange, to blue, to greenish-blue at pH 3.0, 4.0-6.0, 7.0 and 9.0-11.0, respectively. The color changes of the extract solutions over various storage periods were determined using UV/Vis spectra these color changes indicate characteristic absorption patterns and a discoloration index which indicates the rate of absorbance (532 nm/454 nm). Methionine addition influenced the storage stability of the colorant solutions and this addition led to better storage stability than non-addition. In paper chromatography of juice extracted colorant, a long stripe was seen on development. Among three colorants obtained via paper chromatography according to development rate, at least two different colorants were mixed indicated by the appearance, or not, of a shoulder at 552 nm depending on the extent of development. |
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| Ű¿öµå |
| Black cowpea;Juice extractor system;Methionine;Natural colorant; |
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Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸÁö / v.24, no.5, 2011³â, pp.499-506
Çѱ¹ÀÚ¿ø½Ä¹°ÇÐȸ
ISSN : 1226-3591
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO201130856167951)
¾ð¾î : Çѱ¹¾î |
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| ³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø |
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