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한국잔디학회 / v.11, no.1, 1997년, pp.45-58 
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Synechocystis sp. PCC6803을 이용한 Photosystem I- mutants의 색소 및 틸라코이드막 단백질 분석
( Analysis of Pigments and Thylakoid Membrane Proteins in Photosystem I - Mutants from Synechocystis sp. PCC6803 ) |
| 전은경;장남기; 서울대학교 생물교육과;서울대학교 생물교육과;
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| 초 록 |
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| Pigments and thylakoid membrane proteins were investigated in wild type and PS I- mutants from Synechocystis sp. PCC6803 Comparing morphological features, B2 was less fluorescent than the other strains. The contents of chlorophyll a were propotional to the FNR activity in thylakoid membrane. The FNR activity of mutants was lower than that of wild type. In the result of pigments analysis, mutants had smaller cholophyll a than that of wild type. The major carotenoid was found to he $eta$-caroene, but aeaxanthin was barely detected in thylakoid membrane of mutants. The polypeptide, 14.8kD was detected by electrophoresis in mutants. It was considered to be the modification of 15.4kD in wild type. Membrane polypeptides of 17.6 and 19.7kD were not detected in mutants. In the result of western blotting, subunit I was detected in all strains, but subunit II was barely detected in mutants. Subunit II was not detected in B2 at all. In view of the results so far achieved, the changes of contents of chlorophyll and zeaxanthin were affected by the defficiency or modification of functional domain in subunit I. Also the modification in subunit I affected the subunit II- binding site in PS I. As the result, efficiency of photosynthesis was decreased. Key words: Synechoystis sp. PCC6803, PS I - mutant, Photosynthetic efficiency, Pigment,Thylakoid membrane proteins, Subunit I, II. |
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| 키워드 |
| Synechocystis sp. PCC6803;PS I-mutant;Photosynthetic efficiency;Pigment;Thylakoid membrane proteins;Subunit I;II; |
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한국잔디학회지 / v.11, no.1, 1997년, pp.45-58
한국잔디학회
ISSN : 1229-3253
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199711920302381)
언어 : 한국어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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