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한국잔디학회 / v.12, no.4, 1998년, pp.195-202 
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Zoysiagrass japonica의 효율적인 재분화체계에 관한 연구
( High Efficiency of Plant Regeneration from Seed-Derived Callus of Zoysiagrass cv. Zenith ) |
| ;이종민;김두환; 건국대학교 농업생명과학대학 원예과학과;건국대학교 농업생명과학대학 원예과학과;건국대학교 농업생명과학대학 원예과학과;
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| 초 록 |
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| The development of a protocol for high efficiency of embryogenic callus separation, maintenance and plant regeneration from the seeds of zoysiagrass cv. Zenith was studied. Embryogenic callus ratio is absolutely determined by genotype, but by adding high concentration of copper into medium, changing light condition and maintaining callus on initial induction medium for 8∼10 weeks, embryogenic callus can be easily distinguished and its growth can be promoted. There were significant differences among selected callus lines (each from one seed) according to their growth rates and regeneration percentages. Callus pre-treatment with activated charcoal inhibited callus growth, increased the level of precocious germination during culture and promoted shoot cluster formation after transfer to regeneration medium. For long-term callus maintenance, N6AA medium was better than MS medium, because the former inhibited non-embryogenic callus formation and kept vigor of embryogenic callus. The best callus lines Z-(5) has been successfully used for transformation and somaclonal variation selection in our laboratory. |
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| 키워드 |
| zoysiagrass;Zenith;embryogenic callus;regeneration; |
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한국잔디학회지 / v.12, no.4, 1998년, pp.195-202
한국잔디학회
ISSN : 1229-3253
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199811920644333)
언어 : 한국어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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