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한국잔디학회 / v.15, no.1, 2001년, pp.9-14 
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유전자총을 이용한 잔디 형질전환 체계 확립
( Establishment of Transformation Systems of Zoysiagrass by Particle Bombardment ) |
| 임선형;강병철;남궁용;신홍균; 삼성에버랜드(주) 잔디.환경연구소;삼성에버랜드(주) 잔디.환경연구소;삼성에버랜드(주) 잔디.환경연구소;삼성에버랜드(주) 잔디.환경연구소;
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| 초 록 |
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| Callus formation and plant regeneration from the seeds of zoysiagrass cv. Zenith was tested on MS basal medium supplemented with various concentrations of 2,4-dichlorophenoxyacetic acid(2,4-D) and of several cytokinins. A concentration of 1mg/L 2,4-D on medium stimulated callus formation. In the presence of 5mg/L 2,4-D, addition of 1mg/L kinetin significantly enhanced callus formation and plant regeneration over 2,4-D alone. To transfer foreign DNA into turfgrass, parameters for the bombardment of embryogenic callus with the particle bombardment were partially optimized using transient expression assay of a $chimeric eta$-glucuronidase(GUS) gene driven by the CaMV 35S promoter. GUS gene was strongly expressed at helium pressure 1,100 psi and 6~9cm target distance. |
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| 키워드 |
| particle bombardment;transformation;2,4-D;GUS; |
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한국잔디학회지 / v.15, no.1, 2001년, pp.9-14
한국잔디학회
ISSN : 1229-3253
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200111920644263)
언어 : 한국어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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