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한국잔디학회 / v.18, no.4, 2004년, pp.211-219 
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( Herbicide Resistant Turfgrass(Zoysia japonica cv. 'Zenith') Plants by Particle bombardment-mediated Transformation ) |
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| 초 록 |
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| Transgenic zoysiagrass (Zoysia japonica cv. Zenith) plants have been obtained by particle bombardment of embryogenic callus with the plasmid pSMABuba, which contains hygromycin resistance (hpt) and bialaphos resistance (bar) genes. Parameters on DNA delivery efficiency of the particle bombardment were partially optimized using transient expression assay of a chimeric $eta-glucuronidase$(gusA) gene driven by the CaMV 35S promoter. Stably transfarmed zoysiagrass plants were recovered with a selection scheme using hygromycin. Transgenic zoysiagrass plants were confirmed by PCR analysis with specific primer for bar gene. Expression of the transgene in transformed zoysiagrass plants was demonstrated by Reverse transcriptase (RT)-PCR analysis. All the tested transgenic plants showed herbicide BastaR resistance at the field application rate of $0.1%-0.3%$. |
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| 키워드 |
| Basta;herbicide resistance;particle bombardment;turfgrass transformation; |
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한국잔디학회지 / v.18, no.4, 2004년, pp.211-219
한국잔디학회
ISSN : 1229-3253
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200420828899263)
언어 : 영어 |
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| 논문 제공 : KISTI 한국과학기술정보연구원 |
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