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Çѱ¹ÀܵðÇÐȸ / v.23, no.2, 2009³â, pp.345-352
Çѱ¹µéÀܵð ¹è¾Æ¼¼Æ÷ÀÇ ºÎÀ¯¹è¾ç°ú ½Ä¹°Ã¼ Àç»ý
( Embryogenic cell suspension culture and plant regeneration in zoysiagrass (Zoysia japonica Steud) )
ÆØ¿ÞÁÖ¾È;ÇѸ®¹Ù¿ì;ÄûÃæÈÖ;¸®´ÙÀÌ¿¨;¹ÚÅÂÀ±; º£ÀÌ¡ÀÓ¾÷´ëÇб³ Àܵ𿬱¸¼Ò;º£ÀÌ¡ÀÓ¾÷´ëÇб³ Àܵ𿬱¸¼Ò;º£ÀÌ¡ÀÓ¾÷´ëÇб³ Àܵ𿬱¸¼Ò;³ë½º´ÙÄÚ´Ù´ëÇб³ ½Ä¹°Çаú;¿¬¼¼´ëÇб³ »ý¸í°úÇбâ¼úÇкÎ;
 
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Çѱ¹µéÀܵð´Â ¿îµ¿°æ±âÀåÀ̳ª °ñÇÁ ÄÚ½º µî¿¡ Æø³Ð°Ô ¾²ÀÌ´Â ³­ÁöÇü ÀܵðÀÌ´Ù. ¸¹Àº ǰÁ¾Àº ¿µ¾çü·Î ¹ø½ÄÇÑ´Ù. ÀÌ ¿¬±¸´Â ¹è¾Æ¼¼Æ÷ À¯µµ¿Í ½Ä¹°Ã¼ Àç»ýÀ» À§ÇÑ ÀûÁ¤ ¹è¾ç¾× °³¹ß°ú ¹è¾çÁ¶°ÇÀ» ¿¬±¸ÇÏ¿©, Çѱ¹ÀܵðÀÇ À°Á¾°ú ¹ø½ÄÀ» À§ÇÑ ¼¼Æ÷¹è¾ç ü°è È®º¸¸¦ ¸ñÀûÀ¸·Î ÇÏ¿´´Ù. ±× °á°ú $Cu^{++};2.5mgL^{-1}$¸¦ À¯µµ ¹è¾ç¾×¿¡ ÷°¡ÇÏ´Â °ÍÀÌ Ä¶·¯½º À¯µµ¸¦ À§ÇÑ ÃÖÀû ³óµµ Á¶°Ç ÀÌ¿´À½À» º¸¿©ÁÖ¾ú´Ù. Sub-cultureÀÇ È½¼öÀÇ Áõ°¡´Â Ķ·¯½ºÀÇ ÁúÀ» Çâ»ó½ÃÄ×´Ù. ¼¼Æ÷ ºÎÀ¯¹è¾çÀ» À§ÇÑ ÃÖÀû ¿ë·®Àº 2.5¿¡¼­ 10mL »çÀÌ¿´À¸¸ç º» ¿¬±¸¿¡¼­ 58%ÀÇ ½Ä¹°Ã¼ Àç»ý»êºñÀ²À» º¸¿´´Ù.
Zoysiagrass (Zoysia japonica Steud) is a warm season turfgrass species widely used for sports field and golf courses. Many cultivars are propagated through vegetative methods. This study was conducted to develop an optimum culture medium and culture conditions for embryogenic callus induction and plant regeneration, and to establish a cell suspension culture system for use in zoysiagrass breeding and propagation. The results indicated that adding $Cu^{++}$ at 2.5 mg $L^{-1}$ to the induction medium was optimum for callus induction. Increasing the numbers of sub-culture cycles improved the quality of calli. The optimum dosage for cell suspension culture ranged from 2.5 to 10 mL. The embryogenic callus suspension used in this study had a plant regeneration rate of 58%.
 
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¹è;À¯ÀüÀÚ¿ø;À°Á¾;Àܵð;turfgrass;breeding;embryo;germplasm;
 
Çѱ¹ÀܵðÇÐȸÁö / v.23, no.2, 2009³â, pp.345-352
Çѱ¹ÀܵðÇÐȸ
ISSN : 1229-3253
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200920036086852)
¾ð¾î : ¿µ¾î
³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø
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