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Çѱ¹ÇÏõȣ¼öÇÐȸ / v.32, no.4, 1999³â, pp.288-288
±¹³» È£¼ö¿¡¼­ ¹ß»ýÇÑ ³²Á¶·ùÀÇ microcystin ÇÔ·®°ú µ¶¼ºÆò°¡
( Microcystin Content of Cyanobacterial Cells in Korean Reservoirs and Their Toxicity )
±è¹üö;±èÈ£¼·;¹ÚÈ£µ¿;ÃÖ±¤¼ø;¹ÚÁ¾±Ù; °­¿ø´ëÇб³ ÀÚ¿¬°úÇдëÇРȯ°æ°úÇаú;°­¿ø´ëÇб³ ÀÚ¿¬°úÇдëÇРȯ°æ°úÇаú;ÀϺ»½ÅÁÖ´ëÇÐ ÀÌÇкΠ¹°Áú¼øÈ¯Çаú;°­¿ø´ëÇб³ ÀÚ¿¬°úÇдëÇРȯ°æ°úÇаú;Çѱ¹¼öÀÚ¿ø°ø»ç ¼öÀÚ¿ø¿¬±¸¼Ò;
 
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1996³â°ú 1997³â¿¡ ³²Á¶·ù°¡ ¹ß»ýÇÑ ¿©·¯ È£¼ö¿¡¼­ microcystin-RR, microcystin-LR, microcystin-YRÀÇ ÇÔ·®À» Á¤·®ÇÏ¿´°í, Á㸦 ÀÌ¿ëÇÏ¿© µ¶¼º °Ë»ç¸¦ ½Ç½ÃÇÏ¿´´Ù. ¼Ò¾çÈ£¸¦ Á¦¿ÜÇÑ ¸ðµç È£¼ö¿¡¼­ Miorocystis¼ÓÀÇ Á¾µéÀÌ ¿ìÁ¡ÇÏ¿´À¸¸ç Àüü ³²Á¶·ù ¹ß»ý È£¼öÀÇ ½Ã·á¿¡ ´ëÇÑ µ¶¼Ò °ËÃâÀÇ ºóµµ´Â 85%ÀÌ¿´´Ù. Á¶·ù¼¼Æ÷³» ÃÑ microcystinÀÇ ÇÔ·®Àº $0.29{sim}2.61$ mg/gÀ̾úÀ¸¸ç ¼öÁßÀÇ ³óµµ·Î ȯ»êÇϸé $0.4{sim}21.6;{mu}g/l$ÀÌ¿´´Ù. µ¶¼Ò ÇÔ·®Àº 1996³â º¸´Ù 1997³â¿¡ ³ô¾Ò°í, ¼¼ Á¾·ùÀÇ microcystinÁß microcystin-RRÀÌ °¡Àå ¸¹ÀÌ °ËÃâµÇ¾ú´Ù. °¢ ½Ã·áÀÇ Áã¿¡ ´ëÇÑ $LD_{50}$Àº ÃÑ microcystinÇÔ·®°ú $LD_{50}$ÀÇ »ó°ü°ü°è½Ä¿¡ ÀÇÇØ °è»êµÇ¾ú´Ù. È­Çкм®¿¡ ÀÇÇØ microcystinÀÌ °ËÃâµÈ ½Ã·áÀÇ ÃßÃâ¹°ÀÌ ÁÖÀԵǾúÀ» ¶§ ÁãÀÇ Ä¡»çÈ¿°ú°¡ ³ªÅ¸³­ °ÍÀ¸·Î º¸¾Æ microcystinÇÔ·®ÀÇ È­Çкм®°á°ú¿Í bioassay°á°ú´Â Àß ÀÏÄ¡ÇÏ´Â °ÍÀ¸·Î º¸À̸ç, ¿µ¶ûÈ£ ½Ã·á¸¦ ´ë»óÀ¸·Î ½ÇÃøÇÑ $LD_{50}$Àº °æÇè½ÄÀ¸·Î °è»êµÈ $LD_{50}$°ú ÀÏÄ¡ÇÏ¿´´Ù. ´ë»óÈ£¼öÀÇ $LD_{50}$Àº $27{sim}338;mg/kg$ÀÇ ¹üÀ§·Î¼­ ±¹³»¿Ü¿¡¼­ º¸°íµÈ °Í¿¡ ºñÇÏ¿© ³ôÀº µ¶¼ºÀ» ³ªÅ¸³Â´Ù.
Algal cells samples were collected and microcystin contents were determined in warm seasons of $1996{sim}1997$ in ten large reservoirs which are used for drinking water supply in Korea. High density of Microcystis was observed in all lakes except Lake Soyang where Anabaena dominated. The content of three micyocystin variants (microcystin-RR, -YR, -LR) in algal cells was analysed with HPLC and acute toxicity was tested by mouse bioassay. Microcystin-RR was the main component of microcystins variants. The total content of microcystins ranged from 288 to $2612;{mu}g/g$ which corresponds to the concentration per water volume of 0.4 to $21.6;{mu}g/L$. Most samples collected in 1997 showed higher toxin contents than those of 1996. In mouse bioassay, lethal effect was observed in Lake Andong (1990), L. Hapchon (1997), L, Okjong (1997) and Kyounganchon (1996). The livers of mice which died after injection of algal extract were bigger than those of healthy mice, which indicated the presence of hepatotoxin. The frequency of toxic bloom was 85% of total samples. The $LD_{50}$ to mouse were estimated from the empirical relationship between $LD_{50}$ and total microcystin content. The $LD_{50}$ measured with mouse bioassay for one of the samples well coincided with the estimated $LD_{50}$. Estimated $LD_{50}$ of samples ranged from 27 to 338 mg/kg, which shows similar toxicity with reports from other countries.
 
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Cyanobacteyia;Micyocystin;Bioassay;$LD_{50}$;
 
Çѱ¹ÇÏõȣ¼öÇÐȸÁö / v.32, no.4, 1999³â, pp.288-288
Çѱ¹ÇÏõȣ¼öÇÐȸ
ISSN : 1976-8087
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO199910102459587)
¾ð¾î : Çѱ¹¾î
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