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Çѱ¹ÇÏõȣ¼öÇÐȸ / v.35, no.4, 2002³â, pp.326-330
½ÀÁö Åä¾ç¿¡¼­ ü¿ÜÈ¿¼ÒÀÇ ±Ù¿ø°ú º¯È­
( Sources and Variations of Extracellular Enzymes in a Wetland Soil )
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½ÀÁöÅä¾çÀ» µÎ °¡Áö ¹æ¹ýÀ¸·Î ¸ê±ÕÇÑ ÈÄ, ¹Ì»ý¹° È¿¼ÒȰ¼ºµµÀÇ º¯È­¸¦ ¾Ë¾Æº¸¾Ò´Ù. ´Ü±â ¸ê±ÕÀÇ È¿°ú´Â Åç·ç¿£À» °¡ÇÏ¿© ¾Ë¾Æ º¸¾Ò°í, Àå±âÀûÀÎ È¿°ú´Â °¨¸¶¼± Á¶»ç¸¦ ÀÌ¿ëÇÏ¿´´Ù. ó¸®µÈ ½Ã·á¿¡¼­ ${eta}$-glucosidase, ${eta}$-xylosidase, cellobiohydrolase, phosphatase, arylsulfatase, N -acetylglucosaminidase Ȱ¼ºµµ¸¦ methylumbelliferyl °è¿­ÀÇ Àΰø±âÁúÀ» »ç¿ëÇÏ¿© ÃøÁ¤ÇÑ ÈÄ °ø½Ã·á¿Í ºñ±³ÇÏ¿´´Ù. Åç·ç¿£À» °¡ÇÑ °æ¿ì È¿¼Ò¿¡ µû¶ó ´Ù¸¥ ¹ÝÀÀÀ» º¸¿´´Ù. ¿¹¸¦ µé¾î, 󸮿¡ ÀÇÇÏ¿©phosphataseÀÇ È°¼ºÀº Áõ°¡ÇÏ¿´À¸³ª ¹Ý´ë·Î ${eta}$-glucosidase¿Í arylsulfatasemÀÇ È°¼ºµµ´Â °¨¼ÒÇÏ¿´´Ù. °¨¸¶¼± Á¶»ç´Â ¸ðµç È¿¼ÒÀÇ È°¼ºÀ» 40-80%Á¤µµ °¨¼Ò½ÃÄ×´Ù. º» ¿¬±¸ÀÇ Àü¹ÝÀûÀÎ °á°ú¿¡ ÀÇÇϸé, Àû¾îµµ ´Ü±âÀûÀ¸·Î´Â ´Ù·®ÀÇ È¿¼Ò°¡ ¹Ì»ý¹° ü¿Ü¿¡¼­ ¾ÈÁ¤ÀûÀ¸·Î Á¸ÀçÇϳª, Àå±âÀûÀ¸·Î´Âde-novo ÇÕ¼ºÀÌ Áß¿äÇÔÀ» ¾Ë ¼ö ÀÖ¾ú´Ù.
A wetland soil was sterilised by two methods and changes in microbial enzyme activities were assessed. The short-term effects were determined by toluene addition, while the longer-term effects of elimination was monitored by ${gamma}$-radiation. The changes in ${eta}$- glucosidase, ${eta}$-xylosidase, cellobiohydrolase, phosphatase, arylsulphatase, and N-acetylglucosaminidase activities were determined by using methylumbelliferyl model substrates and comparing with the activities of control samples. Toluene addition induced different responses of enzymes. For example, phosphatase activity increased by the treatment while ${eta}$-glucosidase and arylsulphatase activities decreased. In contrast, ${gamma}$-radiation decreased all enzyme activities compared to control by 40-80%. The overall results of the toluene and ${gamma}$-radiation experiments indicate that the large amounts of enzymes are stabilised outside of living cells, at least in the short term, but that the persistence of enzymes is maintained by de-novo synthesis of microbes.
 
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soil enzyme;sterilisation;peatland;bacteria;
 
Çѱ¹ÇÏõȣ¼öÇÐȸÁö / v.35, no.4, 2002³â, pp.326-330
Çѱ¹ÇÏõȣ¼öÇÐȸ
ISSN : 1976-8087
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200218317180491)
¾ð¾î : ¿µ¾î
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