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Çѱ¹ÇÏõȣ¼öÇÐȸ / v.37, no.2, 2004³â, pp.241-247
ºÎ¿µ¾ç È£¼ö¿¡¼­ ºÐ¸®ÇÑ Acidovorax temperans AK-05ÀÇ Anabaena cylindrica ºÐÇØ Ư¼º
( Characterization of a Novel Alga-Lytic Bacterium, Acidovorax temperans AK-05, Isolated from an Eutrophic Lake for Degradation of Anabaena cylindrica )
±èÁ¤µ¿;ÇѸí¼ö; ÇѾç´ëÇб³ »ý¸í°úÇаú;ÇѾç´ëÇб³ »ý¸í°úÇаú;
 
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ºÎ¿µ¾ç È£¼ö·ÎºÎÅÍ »ìÁ¶ ¼¼±ÕÀ» ºÐ¸®ÇÏ°í µ¿Á¤ÇÑ °á°ú Anabaena cylindrica NIES-19¸¦ À¯¸± ź¼Ò¿øÀ¸·Î ÀÌ¿ëÇÏ´Â double layer¹æ¹ýÀ¸·Î 15Á¾ÀÇ »ìÁ¶¼¼±ÕÀ» ºÐ¸®ÇÏ¿´À¸¸ç ³ôÀº »ìÁ¶ Ȱ¼ºÀ» ³ªÅ¸³»´Â 4Á¾ÀÇ »ìÁ¶¼¼±Õ AK-05, AK-07, AK-13 ±×¸®°í AK-28À» ¼±º°ÇÏ¿© »ìÁ¶ ´É·ÂÀ» ºñ±³ÇÏ¿´´Ù. À̵é Áß¿¡ AK-05°¡ °¡Àå ³ôÀº »ìÁ¶ Ȱ¼ºÀ» ³ªÅ¸³»¾úÀ¸¸ç À̸¦ 16S rDNA¿°±â¼­¿­À» ºÐ¼®ÇѰá°ú Acidovorax temperans¿Í 99.5%ÀÇ À¯»ç¼ºÀ» ³ªÅ¸³»¾î Acidovorax temperans AK-05·Î ¸í¸íÇÏ¿´´Ù. A. temperans AK-05ÀÇ ¹è¾ç ¿©¾×À» A. cylindrica NIES-19¿¡ ¶Ñ·ÇÇÑ »ìÁ¶ Ȱ¼ºÀ» ³ªÅ¸³»¾úÀ¸¸ç, À̰ÍÀÇ »ìÁ¶ Ȱ¼º ´É·ÂÀ» ºÐ¼®ÇÑ °á°ú »ìÁ¶ Ȱ¼º¿¡ °ü¿©ÇÏ´Â ÁÖ¿ä ¹°ÁúÀº non-proteinÀÌ¸ç ¿­¿¡ ¾ÈÁ¤ÀûÀ̾ú´Ù. ÀÌ·¯ÇÑ »ìÁ¶ Ȱ¼º ´É·ÂÀº ¾ËÄ®¸® Á¶°Ç°ú 25${sim}$$30^{circ}C$¿¡¼­ °¡Àå ³ô°Ô ³ªÅ¸³Â´Ù. µû¶ó¼­ ÀÌ¿Í °°Àº Ư¼ºÀº ÀϹÝÀûÀ¸·Î ¾ËÄ®¸® Á¶°ÇÀ» ¾ß±âÇÏ´Â Cyanobacteria¿¡ ÀÇÇÑ water bloomsÀÌ ¹ß»ýÇϴ ȣ¼ö¿¡ Àû¿ëÇϴµ¥ ¸Å¿ì À¯¸®ÇÒ °ÍÀ¸·Î ¿©°ÜÁø´Ù.
Isolation and identification of alga-lytic bacteria were carried out. Fifteen isolates of alga-lytic bacteria were screened by the double layer method using A. cylindrica NIES-19 as a sole nutrient and four isolates among them were compared with their alga-lytic activity. The isolate AK-05 exhibiting the highest alga-lytic activity was identified as Acidovorax temperans base on its 16S rDNA sequence. The culture supernatant of the isolate AK-05 was reliable for the alga-lytic. Alga-lytic activity assays of culture supernatant revealed that the major substances for alga-lytic activity were non-proteins and heat stable. The highest alga-Iytic activity was practical under alkaline conditions and at 25${sim}$$30^{circ}C$. It is indicating an advantage for the application of water blooms by cyanobacteria in eutrophic lakes where the pH is generally in alkaline region.
 
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alga-lytic bacteria;Acidovorax temperans;extracellular substances;algal blooms;
 
Çѱ¹ÇÏõȣ¼öÇÐȸÁö / v.37, no.2, 2004³â, pp.241-247
Çѱ¹ÇÏõȣ¼öÇÐȸ
ISSN : 1976-8087
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200418317182860)
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