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Çѱ¹ÇÏõȣ¼öÇÐȸ / v.38, no.4, 2005³â, pp.454-460
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$CO_2$ ½ºÆ®·¹½º¿¡¼ »ýÀåÇÑ °ËÁ¤¸»·ÎºÎÅÍ Phosphoenolpyruvate carboxylase À¯µµ
( Induction of Phosphoenolpyruvate Carboxylase from Hydrilla verticillata Grown Under $CO_2$ Stress ) |
| ;±è¿ëÀç; ;´ëÁø´ëÇб³ »ý¸í°úÇаú;
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| º» ¿¬±¸´Â $CO_2$ ½ºÆ®·¹½º ÇÏ¿¡¼ »ýÀåÇÑ °ËÁ¤¸»·ÎºÎÅÍ PEPC ´Ü¹éÁú ¹ßÇö¿¡ ´ëÇÑ È¯°æÁ¶Àý ±âÀÛÀ» Àß ÀÌÇØÇϵµ·Ï Çϴµ¥ ±× ¸ñÀûÀÌ ÀÖ´Ù. PEPC ³óµµ¿Í $CO_2$ º¸»óÁ¡°úÀÇ °ü°è¿¡¼, PEPC³óµµ´Â $CO_2$ º¸»óÁ¡ (${Gamma}$)ÀÌ °¨¼ÒÇÒ ¶§ Á÷¼±»óÀ¸·Î Áõ°¡ÇÏ¿´´Ù. ${Gamma}$¸¦ À¯µµÇÏ´Â °úÁ¤ µ¿¾È ¸ÅÀÏ ÃøÁ¤ÇÏ¿´´Ù. °ËÁ¤¸»Àº Ãʱâ 0Àϰú 1ÀÏ »çÀÌ¿¡ ${Gamma}$¸¦ ±Þ°¨½Ã۸ç, ÀÌÈÄ 5ÀϱîÁö´Â ¿Ï¸¸ÇÑ °¨¼Ò¸¦ ³ªÅ¸³»¾ú´Ù. PEPC Ȱ¼ºÀº 0ÀÏ¿¡¼ 5ÀϱîÁö À¯µµ °úÁ¤ µ¿¾È ¸ÅÀÏ Áõ°¡ÇÏ¿´À¸¸ç, 5ÀÏ µ¿¾È 4¹è ÀÌ»ó Áõ°¡ÇÏ¿´´Ù. PEPC´Â 100 KDÀ¸·Î »óºÎ¿¡ µÎ °³ÀÇ band¸¦ ³ªÅ¸³»¾ú´Ù. º» ¿¬±¸ÀÇ °á°ú À¯µµ ½ÃÀÛ ÈÄ 24½Ã°£ ³»¿¡ PEPC ´Ü¹éÁú°ú È¿¼ÒȰ¼ºÀÌ Áõ°¡ÇÏ¿´´Ù. |
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| The goal of this research was to better understand the mechanism of environmental control of PEPC expression from Hydrilla verticillata grown under $CO_2$ stress. When PEPC concentration was plotted against $CO_2$ compensation point (${Gamma}$), a linear increase in PEPC concentration was seen with a decrease in $CO_2$ compensation point. ${Gamma}$ was measured on each day of the time course induction. Hydrilla plants exhibited an initially large decrease in ${Gamma}$ between day 0 and day 1; thereafter, the ${Gamma}$ declined in a fairly linear trend for the remaining 5 day time course. PEPC activity varied greatly over the time course. PEPC activity increased daily from day 0 through day 5 of the time course. Activity of PEPC increased almost 4-fold over the time course induction. The two upper bands with the approximately 100 kD mass are the PEPC subunits and PEPC was barely detectable in day 0 but increased through day 4 with day 5 being approximately equal to day 4. We provide evidence of increased PEPC protein concentration, and increased PEPC enzyme activity in as little as 24 h after beginning of induction. |
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| Ű¿öµå |
| Hydrilla verticillata;environmental induction;phosphoenolpyruvate carboxylase; |
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Çѱ¹ÇÏõȣ¼öÇÐȸÁö / v.38, no.4, 2005³â, pp.454-460
Çѱ¹ÇÏõȣ¼öÇÐȸ
ISSN : 1976-8087
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200518317185588)
¾ð¾î : ¿µ¾î |
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| ³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø |
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