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Çѱ¹ÇÏõȣ¼öÇÐȸ / v.39, no.2, 2006³â, pp.219-225
FISH¹ýÀ¸·Î ºÐ¼®ÇÑ ÁÖ¾ÏÈ£ÀÇ ¼¼±Õ±ºÁý±¸Á¶
( Analysis of Bacterial Community Structure Using FISH in the Juam Reservoir )
¹ÚÁöÀº;¿©»ó¹Î;ÀÌ¿µ¿Á; ´ë±¸´ëÇб³ »ý¸í°úÇкÎ;´ë±¸´ëÇб³ »ý¸í°úÇкÎ;´ë±¸´ëÇб³ »ý¸í°úÇкÎ;
 
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ºó¿µ¾çÈ£¿¡¼­ ºÎ¿µ¾çÈ£·Î ÀüÀ̰úÁ¤¿¡ ÀÖ´Â ÁÖ¾ÏÈ£¿¡¼­ ¹°Áú¼øÈ¯°ú ¿¡³ÊÁöÈ帧¿¡ Áö´ëÇÑ ¿ªÇÒÀ» ´ã´çÇÏ´Â ¼¼±Õ ±ºÁý±¸Á¶¸¦ ºÐ¼®Çϱâ À§ÇØ ¼ö°è¿¡ ÁÖ·Î Á¸ÀçÇÏ´Â ${alpha};{cdot};{eta};{cdot};{gamma}$-subclass proteobacteria¿Í Cytophaga-Flavobacterium (CF) group ¼¼±Õ±º, ±×¸®°í Áú¼Ò¼øÈ¯¿¡ °ü¿©ÇÏ´Â ÁúÈ­¼¼±ÕÀ» FISH¹ýÀ¸·Î 2005³â 4ÀϺÎÅÍ 2006³â 1¿ù±îÁö 4ȸ¿¡ °ÉÃÄ °ËÃâÇÏ¿´´Ù. ÃѼ¼±Õ¼ö´Â ¿±·Ï¼Ò-a³óµµ°¡ ³ôÀ» ¶§ ¸¹ÀÌ °ËÃâµÇ¾ú°í ¿±·Ï¼Ò-a³óµµ°¡ ³·À» ¶§´Â °¨¼ÒÇÏ´Â °æÇâÀ» º¸¿´´Ù. ¼ö°è¿¡ Á¸ÀçÇÏ´Â ¹°Áú¼øÈ¯¿¡ °ü¿©ÇÏ´Â ¼¼±ÕµéÀÇ µ¿Å¸¦ ÆÄ¾ÇÇϴµ¥ ¼Ó(genus)¼öÁØÀÇ ¼¼±Õ±ºÀÌ ¾Æ´Ñ ±×·ìº°¼¼±Õ, Áï ${alpha};{cdot};{eta};{cdot};{gamma}$-subclass proteobacteria¿Í Cytophaga-Flavobacterium (CF) group ¼¼±Õ±ºÀ» µ¿Á¤ÇÏ¿© Á¤·®È­ÇÏ¸é Æ¯Á¤ ¼¼±Õ±ºµé¿¡ °üÇÑ ¼¼ºÎÀûÀÎ Á¤º¸´Â ¾òÀ» ¼ö ¾øÁö¸¸ ÇØ´ç¼ö°èÀÇ °Å½ÃÀûÀÎ ÀÚÁ¤ÀÛ¿ë Á¤µµ¸¦ ÆÄ¾ÇÇÒ ¼ö ÀÖ´Â Á¤º¸¸¦ ¾òÀ» ¼ö ÀÖ´Ù. ±× º¯È­¾ç»óÀ» º¸¸é ºó¿µ¾ç »óÅÂÀÇ È£¼ö¿¡¼­ ÁÖÁ¾À» ÀÌ·ç´Â ${alpha};{cdot};{eta}$-subclass proteobacteriaÀÌ 4¿ù¿¡ °¡Àå ¸¹ÀÌ °ËÃâµÇ¾ú°í ³²¼¼±Õ µîÁ¶·ùÀÇ ¹ø½ÄÀÌ ¿Õ¼ºÇß´ø 6${sim}$9¿ù¿¡ °¨¼ÒÇÏ´Â ¾ç»óÀ» ³ªÅ¸³Â´Âµ¥ ÀÌ´Â ÀÌ ½Ã±â¿¡ ¿ìÁ¡ÇÏ´ø ³²¼¼±Õ, M. aeruginosaÀÌ »ý¼ºÇÏ´Â µ¶¼º¹°ÁúÀÌ ÀÌµé ¼¼±ÕÀÇ ¹°Áú´ë»ç¸¦ ÀúÇØÇϱ⠶§¹®À¸·Î »ý°¢µÈ´Ù. ¶ÇÇÑ ºÐÇØ°¡ ¿ëÀÌÇÑ ÀúºÐÀÚ À¯±â¹°À» ÀÌ¿ëÇØ »¡¸® ¼ºÀåÇÏ¸ç ¹èÁö·Î ºÐ¸®¹è¾çÀÌ °¡´ÉÇÑ ´ëºÎºÐÀÇ Á¾¼Ó¿µ¾ç¼¼±Õ(heterotrohs)À» Æ÷ÇÔÇÏ´Â ${gamma}$-subclass proteobacteriaµµ Á¶·ùÀÇ ¹ø½ÄÀÌ ¿Õ¼ºÇÏ´ø 6${sim}$9¿ùº¸´Ù´Â 4¿ù°ú 1¿ù¿¡ ³ôÀº ºñÀ²·Î °ËÃâµÇ¾ú´Âµ¥ ÀÌ´Â À̽ñ⿡ ÁÖ¾ÏÈ£¿¡ Á¸ÀçÇÏ´Â À¯±â¹°ÀÇ ºÐÇØ ¹× ÀÚÁ¤ÀÛ¿ë¿¡±âÀÎÇÏ´Â °ÍÀ¸·Î »ç·áµÈ´Ù. ¹Ý¸é CF group¼¼±Õ±ºÀº 1¿ùÀ» Á¦¿ÜÇϰí´Â °ÅÀÇ À¯»çÇÑ ºñÀ²·Î °üÂûµÇ¾ú´Ù. ¾Æ¿ï·¯ ÁúÈ­¼øÈ¯¿¡ °ü¿©ÇÏ´Â ÁúÈ­¼¼±Õµµ µ¿Àý±â¿¡ Áõ°¡ÇÏ´Â ¾ç»óÀ» º¸¿´À¸¸ç À̶§ ¼ö°èÀÇ ³ôÀº DO°¡ ±×µéÀÇ È°¼º¿¡ ÁÖ¿äÀÎÀ¸·Î ÀÛ¿ëÇÏ´Â °ÍÀ¸·Î »ç·áµÈ´Ù.
Bacterial community structure in the Juam Reservoir was analysed using fluorescent in situ hybridization (FISH) technique from April 2005 to January 2006. Total bacterial numbers varied in the range of 1.58 ${sim};2.73{ imes};10^6;cells;mL^{-1}$ proportional to the concentration of chi-a and had the minimal value in January. The ratios of ${alpha};{cdot};{eta};{cdot};{gamma}$-subclass proteobacteria and Cytophaga-Flavobacterium (CF) group to total bacteria ranged from 45.1% to 77.5%, and the ratios of ${alpha};{cdot};{eta};{cdot};{gamma}$-subclasses to total bacteria in June and September with the concentration of chi-a measured were lower than those ratios in April and January. It suggests that enriched growth of Microcystis aeruginosa may inhibit the metabolic activlty of ${alpha};{cdot};{eta};{cdot};{gamma}$-subclass proteobacteria. However, the ratio of CF group bacteria represented no large change depending on algal bloom. In terms of nitrifying bacteria, the numbers of ammonia-oxidizing bacteria ranged from 9.9 ${ imes};10^4;to;25.5;{ imes}10^4;cells;mL^{-1}$ with sharp fluctuation whereas those of nitrite-oxidizing bacteria varied in 8.7${sim}9.8{ imes}10^4;cells;mL^{-1}$ without noticeable change except the maximal value of $20.3{ imes}10^4;cells;mL^{-1}$ in January maybe due to the high DO.
 
Ű¿öµå
Juam Reservoir;FISH;proteobacteria/CF-group;nitrifying bacteria;
 
Çѱ¹ÇÏõȣ¼öÇÐȸÁö / v.39, no.2, 2006³â, pp.219-225
Çѱ¹ÇÏõȣ¼öÇÐȸ
ISSN : 1976-8087
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200618317186962)
¾ð¾î : Çѱ¹¾î
³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø
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