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Çѱ¹ÇÏõȣ¼öÇÐȸ / v.39, no.2, 2006³â, pp.209-218
µ¿¹°Çöûũſ ³ëÃâ °­µµ°¡ À¯Çس²Á¶ Microcystis aeruginosa¿Í Planktothrix agardhiiÀÇ »ýü·® ¹× ¼¼Æ÷³» microcystinÇÔ·®º¯È­¿¡ ¹ÌÄ¡´Â ¿µÇâ
( Effect of Zooplankton Exposures on the Biomass and Intracellular Microcystin in Microcystis aeruginosa and Planktothrix agadhii )
Àå¹ÎÈ£;Á¤Á¾¹®;ÁÖ±âÀç; ÀϺ»±¹¸³È¯°æ¿¬±¸¼Ò;ºÎ»ê½Ã »ó¼öµµ»ç¾÷º»ºÎ ¼öÁú¿¬±¸¼Ò;ºÎ»ê´ëÇб³ »ý¹°Çаú;
 
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µÎ Á¾ÀÇ Ãʽļº µ¿¹°Çöûũſ(Daphnia magna. Moina macrocopa)ÀÇ Á÷Á¢³ëÃ⠹еµÂ÷(0, 4, 8°³Ã¼)¿Í µ¿¹°Çöûũſ ¹è¾ç¿©°ú¾×(ZCMF) ³óµµÂ÷(0, 25, 50%)¿¡ µû¸¥, µÎ³²Á¶ Microcystis aeruginosa¿Í Planktothrix agardhiiÀÇ »ýü·®°ú ¼¼Æ÷³» microcystin(MC)À» 1ÀÏ °£°ÝÀ¸·Î ÃøÁ¤ÇÏ¿´°í, Á÷Á¢³ëÃâ½ÇÇèÀÇ °æ¿ì µ¿¹°Çöûũſ »ýÁ¸·ûÀ» 1ÀÏ °£°ÝÀ¸·Î °üÂûÇÏ¿´´Ù. µ¿¹°Çöûũſ Á÷Á¢³ëÃâ ½ÇÇè °á°ú, µÎ ±ÕÁÖ¿¡¼­ ¼¼Æ÷³» MCÇÔ·®ÀÌ Áõ°¡ÇÏ¿´À¸¸ç (rm-ANOVA: P< 0.039), P. agardhii±ÕÁÖ¿¡¼­´Â 4°³Ã¼ 󸮱º(ZT1)º¸´Ù 8°³Ã¼ 󸮱º(ZT2)¿¡¼­ Åë°èÀûÀ¸·Î À¯ÀÇÇÑ ¼öÁØÀ¸·Î ³ôÀº ¼¼Æ÷³» MC°ªÀÌ °üÂûµÇ¾ú´Ù(Tukey test. P<0.082).¼¼Æ÷³» MC°¡ ÃÖ°í°ªÀ» º¸ÀÎ 3ÀÏ ¶Ç´Â 4Àϰ¿¡, 󸮱ºµéÀº ´ëÁ¶±º¿¡ ºñÇÏ¿© Åë°èÀûÀ¸·Î À¯ÀÇÇÑ Â÷À̸¦ º¸¿´´Ù(One-way ANOVA, P< 0.021). ZCMF¿¡ ³ëÃâ½ÃŲ M. aeruginosa±ÕÁÖ¿¡¼­ ¼¼Æ÷³» MCÇÔ·®ÀÌ Åë°èÀûÀ¸·Î À¯ÀÇÇÑ ¼öÁØÀ¸·Î Â÷À̰¡ ÀÖ¾ú´Ù(rm-ANOVA: P< 0.004), ½ÇÇè±â°£µ¿¾È ¼¼Æ÷³» MC°ªÀº M. aeruginosa±ÕÁÖ¿¡¼­ 25%󸮱º º¸´Ù 50%󸮱º¿¡¼­ Åë°èÀûÀ¸·Î À¯ÀÇÇÑ ¼öÁØÀ¸·Î ³ô°Ô ³ªÅ¸³µ´Ù(Tukey test, Plt; 0.025). º»¿¬±¸°á°ú, ¼¼Æ÷³» MC·®Àº Ãʽļº µ¿¹°ÇöûũſÀÇ Á÷Á¢³ëÃ⠹еµÂ÷¿Í ºÐºñÈ­Çй°Áú(infochemical)³óµµÂ÷¿¡ ÀÇÁ¸ÇÏ¿© Áõ°¡ ȤÀº º¯È­µÉ¼ö ÀÖ´Â °¡´É¼ºÀÌ ÀÖ´Â °ÍÀ¸·Î ³ªÅ¸³µÀ¸¸ç, ºÎ¿µ¾çÈ£¿¡¼­ µ¶¼º ³²Á¶ ¹ø¼º Á¶Àý½Ã, ÀÌ·¯ÇÑ °¡´É¼ºÀ» °í·ÁÇÏ¿© »ý¹°Á¶ÀýÀÌ ÀÌ·ç¾îÁ®¾ß ÇÒ °ÍÀ¸·Î º¸ÀδÙ.
This study was to evaluate microcystin production by two strains of cyanobacteria (Microcystis aeruginosa and Planktothrix agardhii) in response to three different levels of direct (0,4,8 inds.) or indirect (0,25, 50% of zooplankton culture media filtrate) exposures to zooplankton (Daphnia magna and Moina macrocopa). The cell biomass and intracellular microcystin (MC) were measured everyday. The survival rates of zooplankton were evaluated for daily intervals for the direct exposure. The intracellular MC produced peaked on the day 3 or 4, and then decreased over the both exposure experiment. In the direct experiment, the MC values were significantly different among the control and zooplankton treatments (ZT; repeated measures-ANOVA: P< 0.039). The MC contents of P. agardhii strain (No.204) were significantly higher (Tukey test, P< 0.082) in ZT2 (8 inds.) than in ZT2 (4 inds.). On the peak day, the intracellular MC exposed to both zooplanktons was significantly higher than the control (One-way ANOVA, P< 0.021). Higher zooplankton survivals were observed in the M. aeruginosa strain (No. 111) rather than in high toxic P. agardhii strain. In the indirect experiment, the intracellular MC of the M. aeruginosa strain was significantly different among the control and zooplankton culture media filtrate (ZCMF)treatments (rm-ANOVA: P<0.004), The MC exposed ZCMF2 (50%) were significantly higher than in ZCMFI (25%: Tukey test, P< 0.025) for both strains. This study strongly supports the induced-defensive MC production of potentially toxic cyanobacteria in response to the presence of zooplankton.
 
Ű¿öµå
Microcystis aeruginosa;Planktothrix agardhii;Daphnia magna;Moina macrocopa;growth;intracellular microcystin;infochemical;
 
Çѱ¹ÇÏõȣ¼öÇÐȸÁö / v.39, no.2, 2006³â, pp.209-218
Çѱ¹ÇÏõȣ¼öÇÐȸ
ISSN : 1976-8087
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200618317186957)
¾ð¾î : Çѱ¹¾î
³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø
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