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Çѱ¹ÇÏõȣ¼öÇÐȸ / v.40, no.1, 2007³â, pp.143-148
±¹³» ÀÚ¿¬»ê ºØ¾î¿Í ÀϺ»¿¡¼­ µµÀÔµÈ ¶±ºØ¾î¸¦ ±¸ºÐÇϱâ À§ÇÑ Á¾Æ¯ÀÌÀû ºÐÀÚ¸¶Ä¿ °³¹ß
( Development of Species-specific Molecular Marker as a Tool for Discrimination between Crucian Carp Gengorobuna (Carassius cuvieri) Introduced from Japan and Korean Native One (C. auratus) )
¼Û±³È«;Á¤Á¾¿ì;±¸Çý¿µ;±è¿ø; ¼­¿ï´ëÇб³ »ý¸í°úÇкÎ;¼­¿ï´ëÇб³ »ý¸í°úÇкÎ;»óÁö´ëÇб³ »ý¸í°úÇаú;¼­¿ï´ëÇб³ »ý¸í°úÇкÎ;
 
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¿Ü·¡ µµÀÔÁ¾Àº ±¹³» »ýŰ迡 ºó¹øÈ÷ ½É°¢ÇÑ ¹®Á¦Á¡µéÀ» ÀÏÀ¸Äѿ԰í, ÀÌ·¯ÇÑ Á¾ ÁßÀÇ Çϳª°¡ ÀϺ»¿¡¼­ µµÀÔµÈ ´ã¼ö¾î·ùÀÎ ¶±ºØ¾î (Carassius cuvieri)ÀÌ´Ù. ±¹³» ´ã¼ö»ýŰ迡¼­ ¶±ºØ¾îÀÇ ÇØ·Î¿î ¿µÇâµéÀ» Æò°¡Çϱâ À§ÇØ ¿ì¼±ÀûÀ¸·Î ¼±ÇàµÇ¾î¾ß ÇÒ ¿¬±¸´Â ÇüÅÂÀûÀ¸·Î °¡Àå À¯»çÇÑ Á¾(Corassius auratus)À¸·ÎºÎÅÍ À̵éÀ» ±¸ºÐÇØ¾ß ÇÏ´Â °ÍÀÌ´Ù. ÀüÅëÀûÀÎ ÇüÅÂÀû µ¿Á¤Àº À̵é Á¾ÀÇ ¸Å¿ì À¯»çÇÑ Ç¥ÇöÇüÀ¸·Î ÀÎÇØ ½Å·ÚÇÒ ¼ö ¾ø´Â °á°úµéÀ» ÀÚÁÖ º¸¿©Áֱ⠶§¹®¿¡ ´õ¿í È¿°úÀûÀÎ ¹æ¹ýÀÌ ÇÊ¿äÇÏ´Ù. À̸¦ À§ÇØ º» ¿¬±¸¿¡¼­´Â 3Á¾·ùÀÇ ÇÁ¶óÀ̸Ó(DDF, DDR ±×¸®°í DDR1)¸¦ ÀÌ¿ëÇÑ È¿À²ÀûÀÎ one-step PCRºÐÀÚ¸¶Ä¿¸¦ °³¹ß Àû¿ëÇÏ¿´´Ù. ÀÌ ºÐÀÚ¸¶Ä¿´Â ±¹³» ´ã¼ö »ýŰèÀÇ ¾î·ù ±ºÁý ¸ð´ÏÅ͸µ¿¡ Áß¿äÇÑ ¿ªÇÒÀ» ÇÒ °ÍÀ¸·Î ±â´ëµÈ´Ù.
The introduced exotic species has often caused severe problems to the native ecosystem. One of such species is the freshwater fish gengorobuna (Carassius cuvieri) introduced from Japan. The first step to assess harmful effects of this species on the Korean freshwater ecosystem is to discriminate it from the most similar native crucian carp (Carassius auratus). Because traditional morphological identification often gives unreliable results due to their highly similar phenotype, a new more efficient method is needed. For this purpose, molecular markers produced by the efficient one-step PCR method using three primers (DDF, DDR and DDR1) were developed and tested in the present study. This molecular marker will play an important role in monitoring fish community of Korean freshwater ecosystem.
 
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introduced species;molecular marker;one-step PCR method;crucian carp;
 
Çѱ¹ÇÏõȣ¼öÇÐȸÁö / v.40, no.1, 2007³â, pp.143-148
Çѱ¹ÇÏõȣ¼öÇÐȸ
ISSN : 1976-8087
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200709905949417)
¾ð¾î : ¿µ¾î
³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø
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