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Çѱ¹ÇÏõȣ¼öÇÐȸ / v.39, no.1, 2006³â, pp.119-130
¿¬¼Ó¹è¾ç¿¡¼­ Microcystis aeruginosaÀÇ Áú¼Ò ÀÎ Èí¼ö¿Í »ýÀå Æ¯¼º
( Nitrogen and Phosphorus Uptake and Growth Kinetics of Microcystis aeruginosa Cultured under Chemostats )
ÀÌ¿ÁÈñ;Á¶°æÁ¦; ÀÎÁ¦´ëÇб³ ³«µ¿°­È¯°æ¿¬±¸¼¾ÅÍ;ÀÎÁ¦´ëÇб³ ȯ°æ°øÇкÎ;
 
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¿¬¼Ó¹è¾çÀÇ ÆòÇü Á¤»ó»óÅ Á¶°Ç¿¡¼­ ´ã¼öÁ¶·ù Microcystis aeruginosaÀÇ ÃÖ´ë»ýÀå·ü (${mu}_m$)°ú ¼¼Æ÷³» ¿µ¾ç¿°Àdzóµµ(Q)ÀÇ °ü°è, Áú¼Ò °áÇÌ »óÅ¿¡¼­ ¾×ü¹èÁö¿¡¼­ ¿µ¾ç¿° ³óµµ(S)¿Í È©¼öÀ²(V)ÀÇ °ü°è¸¦ °¢°¢ Á¶»çÇÏ¿´´Ù. Áú¼ÒÁ¦ÇÑÁ¶°Ç¿¡¼­ M. aeruginosaÀÇ ¼¼Æ÷ ´ç $Q_{0N}$Àº 0.04 pmol $cell^{-1}$. ${mu}_m$Àº 1.1 $day^{-1}$¿´°í, Á¦ÇÑÁ¶°Ç¿¡¼­ ÃÖÀú°ª $Q_{0N}$°ú ÃæºÐÁ¶°Ç¿¡¼­ $Q_N$À» ź¼Ò±âÁØÇÏ¿© °¢°¢ 6.1 ${mu}mol$ mg $C^{-1}$ ¹× 9.5${sim}$12.4 ${mu}mol$ mg $C^{-1}$¿´´Ù. ´Ù¸¥ ¹Ì¼¼Á¶·ù¿Í ºñ±³f'1¸é ¼¼Æ÷ÀÇ Áú¼Ò ÇÔ·®ÀÌ ¸Å¿ì ³ô¾Ò°í Áú¼Ò °áÇ̽ÿ¡µµ ±× ÇÔ·®ÀÌ ³ô°Ô À¯ÁöµÇ¾î Áú¼Ò ¿ä±¸µµ°¡ ³ôÀº Á¾À̾ú´Ù Áú¼Ò Á¦ÇÑÁ¶°Ç¿¡¼­ ¿µ¾ç¿° È©¼ö ÃøÁ¤ °á°ú¿¡¼­ ¹ÝÆ÷È­°è¼ö($K_s$)¿Í ÃÖ´ëÈí¼öÀ² ($V_m$)Àº °¢°¢ 36 ${sim}$ 61 ${mu}M$ ¹× 0.28 ${sim}$ 0.35 ${mu}mol;cell;{cdot};hr^{-1}$ ¹üÀ§¿´´Ù. M. aeruginosaÀÇ $K_5$°ªÀº ¸Å¿ì ³ô¾Ò°í $V_m$µµ ³ôÀº ÆíÀ̾ú´Ù. $K_s$¿Í Èí¼ö·Â $V_m$Àº ¹Ì¼¼Á¶·ùÀÇ ¿µ¾ç»óÅ Áï ¿µ¾ç¿°ÀÇ ¼¼Æ÷³» ÇÔ·®(Q)À̳ª Á¦ÇÑ¿µ¾ç¿°ÀÇ °áÇÌÀÇ °­µµÀÇ ¿µÇâÀ» ¹Þ°í ¹Ì¼¼Á¶·ù »ýÀå±Õ°úµµ °ü·ÃÀÌ ÀÖ´Ù. M. aeruginosa´Â ´Ù¸¥ ¹Ì¼¼Á¶·ù¿¡ ºñÇÏ¿© ¼¼Æ÷³» Áú¼Ò ÇÔ·®°ú »ýÀå·üÀÌ ³ô±â ¸Å¹®¿¡ Áú¼Ò¿Í Àο¡ ´ëÇÑ È¼öÀ²ÀÌ ³ôÀ» °ÍÀÌ´Ù. ±×·¯³ª Áú¼Ò Èí¼ö·ÂÀÇ ¹ÝÆ÷È­°è¼ö $K_s$°ªÀÌ ³ô°í $V_m/K_s$ºñÀ²Àº ³·¾Æ ¼ö°Ô¿¡¼­ Áú¼Ò°¡ Àú³óµµ ÀÏ ¶§¿¡´Â ´Ù¸¥ ¹Ì¼¼Á¶·ù¿Í ºñ±³ÇÏ¸é °æÀï·ÂÀÌ ¶³¾îÁö°í Áú¼Ò¿¡ ´ëÇÑ ±âÁú ģȭ·ÂÀº ¾àÇÑ °ÍÀ¸·Î ³ªÅ¸³µ´Ù. ³«µ¿°­ ÇÏ·ùÁö¿ª¿¡¼­ M. aeruginosa°¡ ´ë¹ß»ýÇÏ´Â ½Ã±â¿¡ ¼öÁß ¿µ¾ç¿°ÀÇ ³óµµ º¯µ¿Àº M. aeruginosaÀÇ ¿µ¾ç»ý¸® kinetics Ư¼º°ú Àß ºÎÇÕÇÏ´Â °ÍÀ¸·Î ³ªÅ¸³µ´Ù.
As unialgal cultures to examine the growth kinetics of an algal species, Microcystis aeruginosa was grown in chemostats with nitrogen and phosphorus limitation. The nutrient concentrations of $NH_4;^+;and;PO_4;^{3-}$ to limit the growth of M, aeruginosa were approximately 200 ${mu}M$ and 7 ${mu}M$, respectively. Cell size of the algae decreased towards the $NH_4$-nitrogen limitation under a constant dilution rate, while it increased in the $PO_4$-limitaion. The cell quota of nitrogen under nitrogen-limited conditions was 6.1 ${mu}mol$ mg $C^{-1}$ and, under nitrogen sufficient conditions, ranged from 9.5 ${mu}mol$ mg $C^{-1}$ to 12.4 ${mu}mol$ mg $C^{-1}$. In addition to the cell quota, the half-saturation constants for nitrogen uptake ($K_s$) and the growth rate (${mu}_m$) was 36 ${sim}$ 61 ${mu}M$ and 0.28 ${sim}$ 0.35 ${mu}mol$ cell ${cdot}$ $hr^{-1}$ to show high values in comparison with other algal species. As the limiting concentration, cell quota and uptake rate of M. aeruginosa were higher than those of any other species, the its nitrogen requirement would be great. In the other side, as the half saturation constant ($K_s$) for nitrogen uptake was higher, and the ratios ofmaximum uptake rate ($V_m$) and $K_s$ was relatively low, the species would have the low competitive ability in the low nitrogen concentration in the ambient water. However, the low concentration of nitrogen in the Nakdong River during the Microcystis outbreak would be the inevitable results of the algal blooms. In the lower Parts of the Nakdong River, the nutrient status was coupled with the growth kinetics of the blooming algae to have clear seasonal variations through a year.
 
Ű¿öµå
chemostat;growth kinetics;Microcystis aeruginosa;N and P limitation;
 
Çѱ¹ÇÏõȣ¼öÇÐȸÁö / v.39, no.1, 2006³â, pp.119-130
Çѱ¹ÇÏõȣ¼öÇÐȸ
ISSN : 1976-8087
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200618317186497)
¾ð¾î : Çѱ¹¾î
³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø
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