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Çѱ¹ÇÏõȣ¼öÇÐȸ / v.43, no.3, 2010³â, pp.409-417
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³³ ³ëÃâ¿¡ µû¸¥ ºØ¾î(Carassius auratus) Á¶Á÷ÀÇ ¹Ì¼¼±¸Á¶ ¹× »ý¸®Àû º¯È
( Morphological and Physiological Effects of Lead (Pb) Exposure on Tissues of Carassius auratus ) |
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| º» ¿¬±¸´Â PbÀÇ ³óµµ¿Í ³ëÃ⠱ⰣÀÌ ºØ¾î(Carassius auratus) Á¶Á÷ÀÇ ¹Ì¼¼±¸Á¶¿Í »ý¸®Àû º¯È¿¡ ¹ÌÄ¡´Â ¿µÇâÀ» Á¶»çÇÏ¿´´Ù. ¾Æ°¡¹Ì, »À¿Í ±ÙÀ° Á¶Á÷¿¡¼ PbÀÇ ÃàÀû ³óµµ´Â ³ëÃâ 40ÀÏ¿¡ Áõ°¡ÇÏ¿´´Ù. ¾Æ°¡¹Ì Á¶Á÷ ³» PbÀÇ ÃàÀû·®Àº ´Ù¸¥ Á¶Á÷¿¡ ºñÇÏ¿© ³ô¾ÒÀ¸¸ç, ¾Æ°¡¹Ì, »À ¹× ±ÙÀ° Á¶Á÷¿¡¼ ³ëÃâ±â°£ÀÌ ±æ¾îÁú¼ö·Ï Áõ°¡ÇÏ¿´´Ù. Pb¿¡ ³ëÃâµÈ ¾Æ°¡¹Ì, »À¿Í ±ÙÀ° Á¶Á÷ÀÇ Ç×»êÈÈ¿¼Ò Ȱ¼ºÀº ¾Æ°¡¹Ì¿Í ±ÙÀ° Á¶Á÷¿¡¼ ³ô¾ÒÀ¸¸ç, ½ÅÀå Á¶Á÷¿¡¼ °¡Àå ³·Àº Ȱ¼ºÀ» º¸¿´´Ù. ¶ÇÇÑ Ç×»êÈÈ¿¼Ò Áß superoxide dismutaseÀÇ È°¼ºÀº ´ëÁ¶±º¿¡ ºñÇØ 2¹è Á¤µµ Áõ°¡ÇÏ¿´À¸³ª, glutathion peroxidasÀÇ È°¼ºÀº ¸Å¿ì ³·¾Ò´Ù. Pb¿¡ ³ëÃâµÈ ¾Æ°¡¹Ì Á¶Á÷¿¡¼ Á¡¾×¼¼Æ÷´Â ¼ö°¡ Áõ°¡ÇÏ¿´À¸¸ç, °ïºÀÈ¿Í ºÎÁ¾ÀÌ ÀÌÂ÷»õº¯¿¡¼ ³ªÅ¸³µ´Ù. ¶ÇÇÑ 40Àϰ£ Pb¿¡ ³ëÃâµÈ ¾Æ°¡¹Ì Á¶Á÷ ³» ¹ÌÅäÄܵ帮¾Æ¿Í ÇÙ¿¡¼´Â ¸·ÀÇ ¼Õ»óÀÌ °üÂûµÇ¾úÀ¸¸ç, ½ÅÀå Á¶Á÷ ³» »ç±¸Ã¼´Â ¼öÃàµÇ¾î º¸¿ì¸¸ ÁÖ¸Ó´ÏÀÇ °ø°£ÀÌ ³Ð°Ô °üÂûµÇ¾ú´Ù. ÀÌ·¯ÇÑ °á°ú·Î º¸¾Æ ºØ¾î Á¶Á÷ ÀÌ Àå±â°£ °í³óµµÀÇ Pb¿¡ ³ëÃâÀÌ µÇ¸é ºñÁ¤»óÀûÀÎ ÇüÅ·Πº¯ÇüµÇ¸ç, ÀÌ¿¡ µû¶ó ¸Å¿ì À¯ÇØÇÑ ¿µÇâÀ» ¹ÞÀ» ¼ö ÀÖÀ» °ÍÀ¸·Î »ý°¢µÈ´Ù. |
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| Present study aimed to investigate morphological and physiological change in the tissues of Carassius auratus after exposure against Pb (lead) with various rearing condition. We measured the level of accumulated heavy metal and analyzed ultrastructure with transmission electron microscopy. The heavy metal, Pb, was accumulated in the gill, bone and integument increased drastically for exposure periods, the 40 days. The accumulation of Pb in the gill showed relatively higher than that in other tissues. And accumulated Pb amounts also were increased with exposure time dependent manner in the gill, bone and integument tissues. Also, specific activities of antioxidation enzymes in all tissue after exposure to Pb were increased in the course of exposure. And the activities of SOD from tissues exposed to Pb were increased 2 folds than those from the unexposed but GPX activities were maintained constant. The increased numbers of mucous cells in gill tissues exposed to Pb were determined and morphological changes, such as clubbing and fusion, were shown secondary lamella. Also, exposure of Pb for 40 days on gills tissues cause membrane damage in mitochondria and nucleus. In kidney tissues, the atrophied glomerulus was observed, and the empty space in Bowman's capsule was expanded. Based on the all results, it is suggested that the exposure to the high level of Pb for long period affect on the morphology of tissues, and change the enzymatic balance in C. auratus. |
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| Carassius auratus;lead;antioxidant enzyme;tissues; |
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Çѱ¹ÇÏõȣ¼öÇÐȸÁö / v.43, no.3, 2010³â, pp.409-417
Çѱ¹ÇÏõȣ¼öÇÐȸ
ISSN : 1976-8087
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO201009654402836)
¾ð¾î : Çѱ¹¾î |
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| ³í¹® Á¦°ø : KISTI Çѱ¹°úÇбâ¼úÁ¤º¸¿¬±¸¿ø |
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