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Çѱ¹È¯°æ»ý¹°ÇÐȸ / v.26, no.2, 2008³â, pp.66-75
Inter Simple Sequence Repeats (ISSR) Ç¥ÁöÀÚ¸¦ ÀÌ¿ëÇÑ Çѱ¹²æÀÇ À¯ÀüÀû ´Ù¾ç¼º ¹× ¾ÆÁ¾°£ÀÇ À¯¿¬°ü°è ºÐ¼®
( Inter Simple Sequence Repeats (ISSR) Marker Analysis of Genetic Diversity in Korean Phasianus colchicus karpowi and Genetic Relationships Among Subspecies of Phasianus spp. )
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Çѱ¹²æ (Korean ring-necked Pheasant, Phasianus colchicus karpowi)°ú ¿Ü±¹ ¾ÆÁ¾ÀÇ À¯ÀüÀû À¯¿¬°ü°è¸¦ ÆÄ¾ÇÇϱâ À§ÇØ ¾ß»ý Çѱ¹²æ, »çÀ° Çѱ¹²æ, »çÀ° Çѱ¹²æ°ú ¿Ü±¹²æ°£ÀÇ ÀâÁ¾²æ, ¿Ü±¹²æ 4¾ÆÁ¾(Áß±¹ ¸µ³Ø, Èæ ¹ÂźƮ, ¹é ¹ÂźƮ, ³ìÄ¡)À» ´ë»óÀ¸·Î ISSR Ç¥ÁöÀÚ ºÐ¼®°ú AMOVA ºÐ¼®À» ¼öÇàÇÏ¿´´Ù. ¾ß»ý Çѱ¹²æÀÇ Àüü À¯Àü ´Ù¾ç¼ºÁß 94.08%°¡ ¼­½ÄÁö ³» °³Ã¼°£ À¯ÀüÀû Â÷ÀÌ¿¡ ±âÀÎÇϰí, 5.9% ($Phi$st=0.059)°¡ ¼­½ÄÁö°£ Â÷ÀÌ¿¡ ±âÀÎÇÏ¿´´Ù. »çÀ° Çѱ¹²æÀÌ À¯ÀüÀûÀ¸·Î ¾ß»ý Çѱ¹²æ º¸´Ù´Â ¿Ü±¹²æ 4¾ÆÁ¾°ú °¡±õ°Ô ³ªÅ¸³µ´Ù. AMOVAºÐ¼®°ú clusterºÐ¼®¿¡¼­ »çÀ° Çѱ¹²æÀÌ ¾ß»ý Çѱ¹²æÀº ¹°·Ð ¿Ü±¹²æ 4¾ÆÁ¾°úµµ À¯ÀüÀû Â÷À̸¦ º¸ÀÌ´Â °ÍÀ¸·Î ¹Ì·ç¾î º¼ ¶§ »çÀ°µÇ°í ÀÖ´Â Çѱ¹²æÀº Çѱ¹²æ°ú ¿Ü±¹ ¾ÆÁ¾°£ÀÇ ´Ù¾çÇÑ ±³Àâ¿¡ ÀÇÇØ »ý°Ü³­ ÀâÁ¾ÀÏ °¡´É¼ºÀÌ ³ô´Ù. ¿Ü±¹²æ 4¾ÆÁ¾(Áß±¹ ¸µ³Ø, Èæ ¹ÂźƮ, ¹é ¹ÂźƮ, ³ìÄ¡)ÀÇ Àüü À¯Àü ´Ù¾ç¼ºÁß 96.63%°¡ ¾ÆÁ¾³» °³Ã¼°£ Â÷ÀÌ¿¡ ±âÀÎÇϰí. 3.4% ($Phi$st=0.034)·Î ¾ÆÁ¾°£ Â÷ÀÌ¿¡ ±âÀÎÇÏ¿© ¿Ü±¹²æ 4¾ÆÁ¾ÀÇ ¾ÆÁ¾°£ À¯ÀüÀû Â÷À̰¡ ¾ß»ý Çѱ¹²æÀÇ ¼­½ÄÁö°£ Â÷À̺¸´Ùµµ ³·Àº °ÍÀ¸·Î ³ªÅ¸³µ´Ù. ÀÌ´Â º» ºÐ¼®¿¡ »ç¿ëµÈ ¿Ü±¹²æ 4¾ÆÁ¾ÀÌ ÇüÅÂÀûÀ¸·Î´Â ´Ù¸¥ ¾ÆÁ¾À¸·Î ºÐ·ùµÇÁö¸¸ À¯ÀüÀûÀ¸·Î´Â ¸Å¿ì °¡±î¿î À§Ä¡¿¡ ÀÖÀ½À» ÀǹÌÇÑ´Ù. 7¼­½ÄÁöÀÇ ¾ß»ý Çѱ¹Á¤°ú ¿Ü±¹²æ 4¾ÆÁ¾À» °¢°¢ ¾ß»ý Çѱ¹²æ ±×·ì°ú ¿Ü±¹²æ ±×·ìÀ¸·Î ºÐ·ùÇÏ¿© µÎ ±×·ìÀÇ À¯ÀüÀû ´Ù¾ç¼ºÀ» ºÐ¼®ÇÑ °á°ú, Àüü À¯Àüº¯ÀÌ Áß ±×·ì°£ Â÷ÀÌÀÇ ºñÀ²Àº 17% ÀÌ»ó(±×·ì ³» ¼­½ÄÁö/¾ÆÁ¾°£ Â÷ÀÌ´Â ¾à 4%)À¸·Î ¾ß»ý Çѱ¹²æÀÌ ¾ÆÁ¾ °ü°èÀÎ ¿Ü±¹²æ 4¾ÆÁ¾°ú »ó´çÇÑ À¯ÀüÀû Â÷À̸¦ º¸ÀÌ´Â °ÍÀ¸·Î ³ªÅ¸³µ´Ù. À¯ÀüÀû À¯¿¬°ü°è ºÐ¼®°á°ú¿¡¼­ Áß±¹ ¸µ³Ø. Èæ ¹ÂźƮ, ¹é ¹ÂźƮÀÇ ¿Ü±¹²æ 3 ¾ÆÁ¾Àº À¯ÀüÀûÀ¸·Î ¿Ü±¹ ¾ÆÁ¾¿¡ °¡±î¿î »çÀ°Çѱ¹²æ, ÀâÁ¾²æ°ú ÇÔ²² ÇϳªÀÇ ºÐÁö±ºÀ» Çü¼ºÇϸ鼭 ¾ß»ý Çѱ¹²æ ±×·ìÀ¸·ÎºÎÅÍ 98% ½Å·Ú¼öÁØ¿¡¼­ ¶Ñ·ÇÇÏ°Ô ±¸º°µÇ¾ú´Ù.
The level of genetic diversity and genetic relationships among Korean ring-necked pheasant (Phasianus colchicus karpowi) habitat and subspecies have been investigated based on Inter Simple Sequence Repeat (ISSR) markers. Wild and domesticated Korean ring-necked pheasant, hybrids between domesticated Korean ring-necked and foreign subspecies, and four foreign subspecies; Chinese ring-necked (P. c. torquatus), Melanistic mutant (P. c. mut. tenebrosus), XL White (P. c. mut) and Southern green (P. c. versicolor) were used for comparison. On the basis of the results of AMOV A, 94.08% of genetic diversity in Korean ring-necked was allocated among individuals within habitat differences. Estimate of $Phi$st, which represents the degree of genetic differentiation among habitats was 5.9%. Based on the dendrogram reconstructed by UPGMA, Yangpyung habitat of the eight habitats turned out to be distinct from others habitat. Interestingly, domesticated Korean ring-necked and hybrid mixture showed closer genetic relationship with four foreign subspecies than Korean ring-necked. As a consequence of AMOVA, 96.63% of genetic diversity in four foreign subspecies was allocated among individuals within subspecies. Estimate of $Phi$st representing the degree of genetic differentiation among subspecies was 3.4%, which was lower than that among habitats of Korean ring-necked. The lower level of genetic difference among four foreign subspecies showed that these subspecies were genetically closer even though they were morphologically classified into four different subspecies. When seven habitats of Korean ring-necked pheasant and four foreign subspecies were divided into Korean and Foreign Pheasant Groups, respectively, more than 17% of genetic diversity was allocated between groups (about 4% among habitats/subspecies within groups). This observation implied that Korean ring-necked pheasant is genetically quite different from four foreign subspecies. On the basis of cluster analysis, three foreign subspecies (Chinese ring-necked pheasant, Melanistic mutant pheasant, and XL White pheasant) formed a distinct group with domesticated Korean ring-necked pheasant and hybrid mixture at 98% confidence interval.
 
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Korean ring-necked pheasant;ISSR;AMOVA;genetic diversity;habitat;Phasianus colchicus karpowi;
 
ȯ°æ»ý¹° / v.26, no.2, 2008³â, pp.66-75
Çѱ¹È¯°æ»ý¹°ÇÐȸ
ISSN : 1226-9999
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200820258463315)
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