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Çѱ¹È¯°æ»ý¹°ÇÐȸ / v.25, no.3, 2007³â, pp.267-272
°íȰ¼º Poly(butylene succinate-co-butylene adipate) ºÐÇØ±ÕÀÇ ¼±¹ß
( Screening of Microorganisms with High Poly (butylene succinate-co-butylene adipate)-Degrading Activity )
±è¸»³²;À̼±Èñ;±è¿Ï±Ô;¿øÇ׿¬; »ó¸í´ëÇб³ »ý¹°Çаú;»ó¸í´ëÇб³ »ý¹°Çаú;³ó¾÷°úÇбâ¼ú¿ø ½Ä¹°º´¸®°ú;³ó¾÷°úÇбâ¼ú¿ø ÀÀ¿ë¹Ì»ý¹°°ú;
 
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¿ì¸®³ª¶ó 20°³ Áö¿ªÀÇ °æÀÛÅä, ¾²·¹±â ¸Å¸³Åä, ºÎ¿±Åä ¹× È°¼º¿À´ÏÅä¿¡¼­ äÃëÇÑ 40°³ Åä¾ç ½Ã·á·ÎºÎÅÍ Poly(butylene succinate-co-butylene adipate) (PBSA)¸¦ ºÐÇØÇÏ´Â ¹Ì»ý¹°À» $37^{circ}C$¿¡¼­ °­È­¹è¾ç°ú Åõ¸íȯ½ÃÇè¹ýÀ» ÀÌ¿ëÇÏ¿© PBSA¸¦ ºÐÇØÇÏ´Â ±ÕÁÖ¸¦ ¼±¹ßÇÏ¿´´Ù. ¼±¹ßÇÑ ¼¼±ÕÀº 16S rDNA ¿°±â¼­¿­ºÐ¼®À¸·Î µ¿Á¤ÇÑ °á°ú Streptomyces sp. PBSA-1·Î ¹àÇôÁ³À¸¸ç Áø±ÕÀº ÇüÅÂÀû, ¹è¾çÀû Ư¡À» ÅëÇÏ¿© Aspergillus fumigatus PBSA-2¿Í Aspergillus fumigatus PBSA-3À¸·Î µ¿Á¤µÇ¾ú´Ù. º¯Çü Sturm test¸¦ ÀÌ¿ëÇÏ¿© ¼±¹ß±ÕÀÇ PBSAºÐÇØÈ°¼ºÀ» ÃøÁ¤ÇÑ °á°ú $37^{circ}C$¿¡¼­ 40ÀÏ µ¿¾È Streptomyces sp. PBSA-1Àº PBSA¸¦ 83% ºÐÇØÇÏ¿´À¸¸ç, A. fumigatus PBSA-2¿Í A. fumigatus PBSA-3Àº PBSA¸¦ °¢°¢ 65% ¹× 75%ºÐÇØÇÏ´Â °ÍÀ¸·Î ³ªÅ¸³ª ÀÌ ±ÕÁÖµéÀº PBSAÀÇ ºÐÇØ¿¡ ´ëÇÏ¿© ¸Å¿ì ³ôÀº Ȱ¼ºÀ» °¡Áö´Â °ÍÀ¸·Î Æò°¡µÇ¾ú´Ù.
Microorganisms capable of degrading poly(butylene succinate-co-butylene adipate) (PBSA) were isolated from 40 soil samples such as landfill site soil, cultivating soil and activated sludge soil from 20 different sites in Korea by using the enrichment culture and the clear zone test at $37^{circ}C$. Based on the 16S rDNA sequences, the isolated bacterium was identified to be Streptomyces sp. PBSA-1. Morphological and cultural characteristics were employed for the identification of the isolated fungi and they were proved to be Aspergillus fumigatus PBSA-2 and Aspergillus fumigatus PBSA-3. The PBSA degradation activity of the isolated microorganisms was enhanced through the serial acclimation in PBSA plate medium. The PBSA degrading microorganisms appeared to be highly active for the PBSA degradation in that 83% of PBSA was degraded by Streptomyces sp. PBSA-l, and 65% and 75% of PBSA was mineralized by A. fumigatus PBSA2 and A. fumigatus PBSA-3 respectively during 40 days of the modified Sturm test.
 
Ű¿öµå
poly(butylene succinate-co-butylene adipate);Streptomyces sp.;Aspergillus fumigatus;degradation activity;
 
ȯ°æ»ý¹° / v.25, no.3, 2007³â, pp.267-272
Çѱ¹È¯°æ»ý¹°ÇÐȸ
ISSN : 1226-9999
UCI : G100:I100-KOI(KISTI1.1003/JNL.JAKO200714364034297)
¾ð¾î : Çѱ¹¾î
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